Results 51 to 60 of about 1,023,137 (333)

Dark side illuminated: imaging of Toxoplasma gondii through the decades. [PDF]

open access: yes, 2013
In the more than 100 years since its discovery, our knowledge of Toxoplasma biology has improved enormously. The evolution of molecular biology, immunology and genomics has had profound influences on our understanding of this ubiquitous bug.
McGovern, Kathryn E, Wilson, Emma H
core   +3 more sources

Evidence for expression of promoterless GFP cassette: Is GFP an ideal reporter gene in biotechnology science?

open access: yesResearch in Pharmaceutical Sciences, 2019
Green fluorescent protein (GFP) has played an important role in biochemistry and cell biology as a reporter gene. It has been used to assess the potency of promoters for recombinant protein production. This investigation reveals evidences suggesting that
Zahra Mohammadi   +4 more
doaj   +1 more source

Water-Soluble Flexible Organic Frameworks That Include and Deliver Proteins. [PDF]

open access: yes, 2020
Four water-soluble hydrazone-based three-dimensional (3D) flexible organic frameworks FOF-1-4 have been synthesized from a semirigid tetracationic tetraaldehyde and four flexible dihydrazides.
Li, Zhan-Ting   +10 more
core  

Genetically Encoded Calcium Indicators: A New Tool in Renal Hypertension Research [PDF]

open access: yes, 2019
Hypertension is ranked as the third cause of disability-adjusted life-years. The percentage of the population suffering from hypertension will continue to increase over the next years.
Schleifenbaum, Johanna, Zhong, Cheng
core   +1 more source

A modular toolset of phiC31-based fluorescent protein tagging vectors for Drosophila

open access: yesFly, 2019
The Drosophila transgenic technology and fluorescent protein fusions are powerful tools to analyze protein expression patterns, subcellular localization and protein dynamics.
Jun Luo, Pingping Shen, Jiong Chen
doaj   +1 more source

Photooxidation-guided ultrastructural identification and analysis of cells in neuronal tissue labeled with green fluorescent protein. [PDF]

open access: yesPLoS ONE, 2013
The ultrastructural characterization of neuronal compartments in intact tissue labeled with green fluorescent protein (GFP) remains a frequently encountered challenge, despite work establishing photooxidation of GFP in cultured cells.
Heinz Horstmann   +2 more
doaj   +1 more source

LIFEGUARD proteins support plant colonization by biotrophic powdery mildew fungi [PDF]

open access: yes, 2013
Pathogenic microbes manipulate eukaryotic cells during invasion and target plant proteins to achieve host susceptibility. BAX INHIBITOR-1 (BI-1) is an endoplasmic reticulum-resident cell death suppressor in plants and animals and is required for full ...
Eichmann, Ruth   +2 more
core   +1 more source

A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins

open access: yesBioTechniques, 2017
Protocol Summary Here we present an ethanol fixation protocol that allows for the direct visualization of the natural fluorescence of reporter proteins while maintaining excellent tissue histology.
Akifumi Nakagawa   +5 more
doaj   +1 more source

Alteration in Fluidity of Cell Plasma Membrane in Huntington Disease Revealed by Spectral Phasor Analysis. [PDF]

open access: yes, 2018
Huntington disease (HD) is a late-onset genetic neurodegenerative disorder caused by expansion of cytosine-adenine-guanine (CAG) trinucleotide in the exon 1 of the gene encoding the polyglutamine (polyQ).
Digman, Michelle A   +3 more
core   +2 more sources

Bright Green Biofluorescence in Sharks Derives from Bromo-Kynurenine Metabolism

open access: yesiScience, 2019
Summary: Although in recent years there has been an increased awareness of the widespread nature of biofluorescence in the marine environment, the diversity of the molecules responsible for this luminescent phenotype has been mostly limited to green ...
Hyun Bong Park   +8 more
doaj   +1 more source

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