Results 31 to 40 of about 1,535 (215)

Getting to grips with ammonium

open access: yeseLife, 2013
A fluorescent sensor that can monitor levels of extracellular ammonium has been made by using a fused green fluorescent protein to detect conformational changes in ammonium transport proteins.
Yi Wang, Jonathan A Javitch
doaj   +1 more source

R26R-GR: a Cre-activable dual fluorescent protein reporter mouse. [PDF]

open access: yesPLoS ONE, 2012
Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level.
You-Tzung Chen   +21 more
doaj   +1 more source

Green Fluorescent Protein: discover, structure, purification, applications, and future prospects

open access: yesJournal of Scientific Research in Science, 2023
Green fluorescent protein (GFP) Firstly discovered from the jellyfish Aequorea victoriaand it was widely used in biological science as a universal fluorescent tag.
Hend A. Anter   +2 more
doaj   +1 more source

Fluorescent proteins reveal what trypanosomes get up to inside the tsetse fly

open access: yesParasites & Vectors, 2019
The discovery and development of fluorescent proteins for the investigation of living cells and whole organisms has been a major advance in biomedical research.
Wendy Gibson, Lori Peacock
doaj   +1 more source

Fluorescence color diversity of great barrier reef corals [PDF]

open access: yesJournal of Innovative Optical Health Sciences, 2015
A group of variously colored proteins belonging to the green fluorescent protein (GFP) family are responsible for coloring coral tissues. Corals of the Great Barrier Reef were studied with the custom-built fiber laser fluorescence spectrometers. Spectral
Grigory Lapshin   +8 more
doaj   +1 more source

Generation of photonic entanglement in green fluorescent proteins

open access: yesNature Communications, 2017
Quantum-enhanced applications such as quantum spectroscopy of biological samples could take advantage from in situ generation of quantum states of light.
Siyuan Shi, Prem Kumar, Kim Fook Lee
doaj   +1 more source

Evolution of a fluorinated green fluorescent protein [PDF]

open access: yesProceedings of the National Academy of Sciences, 2007
The fluorescence of bacterial cells expressing a variant (GFPm) of the green fluorescent protein (GFP) was reduced to background levels by global replacement of the leucine residues of GFPm by 5,5,5-trifluoroleucine. Eleven rounds of random mutagenesis and screening via fluorescence-activated cell sorting yielded a GFP mutant containing 20
Yoo, Tae-Hyeon   +2 more
openaire   +3 more sources

A modular toolset of phiC31-based fluorescent protein tagging vectors for Drosophila

open access: yesFly, 2019
The Drosophila transgenic technology and fluorescent protein fusions are powerful tools to analyze protein expression patterns, subcellular localization and protein dynamics.
Jun Luo, Pingping Shen, Jiong Chen
doaj   +1 more source

Evidence for expression of promoterless GFP cassette: Is GFP an ideal reporter gene in biotechnology science?

open access: yesResearch in Pharmaceutical Sciences, 2019
Green fluorescent protein (GFP) has played an important role in biochemistry and cell biology as a reporter gene. It has been used to assess the potency of promoters for recombinant protein production. This investigation reveals evidences suggesting that
Zahra Mohammadi   +4 more
doaj   +1 more source

Photooxidation-guided ultrastructural identification and analysis of cells in neuronal tissue labeled with green fluorescent protein. [PDF]

open access: yesPLoS ONE, 2013
The ultrastructural characterization of neuronal compartments in intact tissue labeled with green fluorescent protein (GFP) remains a frequently encountered challenge, despite work establishing photooxidation of GFP in cultured cells.
Heinz Horstmann   +2 more
doaj   +1 more source

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