Results 31 to 40 of about 3,922,099 (302)

A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins

BioTechniques, 2015
Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice.
Akifumi Nakagawa, Kate Von Alt, Keith D. Lillemoe, Carlos Fernández-del Castillo, Andrew L. Warshaw, Andrew S. Liss   +5 more
doaj   +1 more source

Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins

Scientific Reports, 2020
In-resin CLEM of Epon embedded samples can greatly simplify the correlation of fluorescent images with electron micrographs. The usefulness of this technique is limited at present by the low number of fluorescent proteins that resist CLEM processing ...
Isei Tanida, Yoko Furuta, Junji Yamaguchi, Soichiro Kakuta, Juan Alejandro Oliva Trejo, Yasuo Uchiyama   +5 more
doaj   +1 more source

Expression of recombinant multi-coloured fluorescent antibodies in gor ^-/trxB^- E. coli cytoplasm [PDF]

, 2011
Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V., Burchell, Joy Marilyn; id_orcid, Kang, Angray S., Anatoliy Markiv, Burchell, J, Durvasula, R.V., Beatson, R., Beatson Richard, Beatson, Richard; id_orcid, Kang Angray S, Markiv, A., Markiv Anatoliy, Kang, A.S., Richard Beatson, Burchell, J., Durvasula Ravi V, Ravi V Durvasula, Burchell, Joy; id_orcid, Durvasula, RV, Markiv, Anatoliy, Burchell Joy, Markiv, A, Angray S Kang, Beatson, Richard, Joy Burchell, Kang, AS, Beatson, R   +26 more
core   +1 more source

A Bright, Nontoxic, and Non-aggregating red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

Frontiers in Cell and Developmental Biology, 2022
Red fluorescent proteins are useful as morphological markers in neurons, often complementing green fluorescent protein-based probes of neuronal activity. However, commonly used red fluorescent proteins show aggregation and toxicity in neurons or are dim.
Lin Ning, Yang Geng, Yang Geng, Matthew Lovett-Barron, Xiaoman Niu, Mengying Deng, Liang Wang, Niloufar Ataie, Alex Sens, Ho-Leung Ng, Shoudeng Chen, Karl Deisseroth, Michael Z. Lin, Michael Z. Lin, Jun Chu   +14 more
doaj   +1 more source

Versatile toolbox for high throughput biochemical and functional studies with fluorescent fusion proteins [PDF]

, 2012
Fluorescent fusion proteins are widely used to study protein localization and interaction dynamics in living cells. However, to fully characterize proteins and to understand their function it is crucial to determine biochemical characteristics such as ...
Sandra B. Hake (165089), Antonia Jack (165084), Sandra B Hake, Patricia Wolf, Pichler Garwin, Pichler, Garwin, Hake, Sandra B., Antonia Jack, Wolf Patricia, Garwin Pichler (165082), Jack Antonia, Wolf, Patricia, Jack, Antonia, Hake Sandra B., Garwin Pichler, Patricia Wolf (165088)   +15 more
core   +2 more sources

Green fluorescent protein: A perspective [PDF]

Protein Science, 2011
AbstractA brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ...
openaire   +2 more sources

Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum

BioTechniques, 2006
O6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins can be specifically and covalently labeled with fluorescent O6-benzylguanine (O6-BG) derivatives for multicolor live cell imaging approaches.
Antje Keppler, Claudio Arrivoli, Lucia Sironi, Jan Ellenberg   +3 more
doaj   +1 more source

Fluorescent proteins as tools to aid protein production

Microbial Cell Factories, 2005
Fluorescent proteins are genetically encoded, highly versatile reporters useful for monitoring various aspects of recombinant protein production. In addition to the widely popular green fluorescent protein (GFP) from Aequorea victoria, a variety of other
Su Wei
doaj   +1 more source

ATP changes the fluorescence lifetime of cyan fluorescent protein via an interaction with His148 [PDF]

, 2010
Contains fulltext : 87235.pdf (Publisher’s version ) (Open Access)Recently, we described that ATP induces changes in YFP/CFP fluorescence intensities of Fluorescence Resonance Energy Transfer (FRET) sensors based on CFP-YFP.
Marieke Willemse, Jalink Kees, Fransen, J.A.M., Jalink, K., Sergey P Laptenok, Krogt, G. van der, Krogt, G., Jan Willem Borst, Laptenok Sergey P., Jack A M Fransen, Slijkhuis Rik, Slijkhuis, R., Willemse, M.P., Fransen Jack A. M., Jack A. M. Fransen, Laptenok, S., Willemse, M., Borst, J.W., Wieringa, B., Borst Jan Willem, Gerard van der Krogt, Rik Slijkhuis, Kees Jalink, Sergey P. Laptenok, van der Krogt Gerard, Laptenok, S.P., Be Wieringa, Wieringa Be, Willemse Marieke   +28 more
core   +3 more sources

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs [PDF]

, 2010
Förster resonance energy transfer (FRET) is a powerful method for obtaining information about small-scale lengths between biomacromolecules. Visible fluorescent proteins (VFPs) are widely used as spectrally different FRET pairs, where one VFP acts as a ...
Birch, D.J.S., Laptenok, S., Visser, N.V., Hoek, A., van, Laptenok, S.P., Borst, J.W., Visser, A.J.W.G., van Hoek, A., Brochon, J.C.   +8 more
core   +1 more source