Results 31 to 40 of about 510,204 (262)
Research in Pharmaceutical Sciences, 2019 Green fluorescent protein (GFP) has played an important role in biochemistry and cell biology as a reporter gene. It has been used to assess the potency of promoters for recombinant protein production. This investigation reveals evidences suggesting that
Zahra Mohammadi +4 more
doaj +1 more source
Photooxidation-guided ultrastructural identification and analysis of cells in neuronal tissue labeled with green fluorescent protein. [PDF]
PLoS ONE, 2013 The ultrastructural characterization of neuronal compartments in intact tissue labeled with green fluorescent protein (GFP) remains a frequently encountered challenge, despite work establishing photooxidation of GFP in cultured cells.
Heinz Horstmann +2 more
doaj +1 more source
Evolution of a fluorinated green fluorescent protein [PDF]
Proceedings of the National Academy of Sciences, 2007 The fluorescence of bacterial cells expressing a variant (GFPm) of the green fluorescent protein (GFP) was reduced to background levels by global replacement of the leucine residues of GFPm by 5,5,5-trifluoroleucine. Eleven rounds of random mutagenesis and screening via fluorescence-activated cell sorting yielded a GFP mutant ...
Yoo, Tae-Hyeon +2 more
openaire +4 more sources
BioTechniques, 2017 Protocol Summary Here we present an ethanol fixation protocol that allows for the direct visualization of the natural fluorescence of reporter proteins while maintaining excellent tissue histology.
Akifumi Nakagawa +5 more
doaj +1 more source
Bright Green Biofluorescence in Sharks Derives from Bromo-Kynurenine Metabolism
iScience, 2019 Summary: Although in recent years there has been an increased awareness of the widespread nature of biofluorescence in the marine environment, the diversity of the molecules responsible for this luminescent phenotype has been mostly limited to green ...
Hyun Bong Park +8 more
doaj +1 more source
A family of GFP-like proteins with different spectral properties in lancelet
Biology Direct, 2008 Background Members of the green fluorescent protein (GFP) family share sequence similarity and the 11-stranded β-barrel fold. Fluorescence or bright coloration, observed in many members of this family, is enabled by the intrinsic properties of the ...
Mushegian Arcady +6 more
doaj +1 more source
Variants of Green Fluorescent Protein GFPxm [PDF]
Marine Biotechnology, 2006 As research progresses, fluorescent proteins useful for optical marking will evolve toward brighter, monomeric forms that are more diverse in color. We previously reported a new fluorescent protein from Aequorea macrodactyla, GFPxm, that exhibited many characteristics similar to wild-type green fluorescent protein (GFP).
Luo, W. X. +6 more
openaire +3 more sources
Personalized Zebrafish Models for Fusion‐Positive Pediatric Sarcomas
Pediatric Blood &Cancer, EarlyView.ABSTRACT Clinical sequencing efforts have revolutionized our approaches to categorizing pediatric cancers in real time. This has dramatically improved our ability to profile pediatric tumors, identify actionable vulnerabilities, and influence clinical care.
Lisa H. Hall +2 more
wiley +1 more source
Sea as a color palette: the ecology and evolution of fluorescence
Zoological Letters, 2020 Fluorescence and luminescence are widespread optical phenomena exhibited by organisms living in terrestrial and aquatic environments. While many underlying mechanistic features have been identified and characterized at the molecular and cellular levels ...
Marie-Lyne Macel +5 more
doaj +1 more source
FEBS Letters, EarlyView.Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi +5 more
wiley +1 more source