Results 81 to 90 of about 3,922,099 (302)

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Ataxin-1 fusion partners alter polyQ lethality and aggregation [PDF]

, 2007
Intranuclear inclusion bodies (IBs) are the histopathologic markers of multiple protein folding diseases. IB formation has been extensively studied using fluorescent fusion products of pathogenic polyglutamine (polyQ) expressing proteins.
Rich, T., Varadaraj, A., Archana Varadaraj, Varadaraj Archana, Tina Rich, Rich Tina   +5 more
core   +2 more sources

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

Fluorescent protein-mediated colour polymorphism in reef corals: multicopy genes extend the adaptation/acclimatization potential to variable light environments

, 2015
The genomic framework that enables corals to adjust to unfavourable conditions is crucial for coral reef survival in a rapidly changing climate. We have explored the striking intraspecific variability in the expression of coral pigments from the green ...
D'Angelo, Cecilia, Oswald, Franz, Wiedenmann, Jörg, Edwards, Richard J., Gittins, John R.   +4 more
core   +1 more source

Green Fluorescent Protein (GFP) color reporter gene visualizes parvovirus B19 Non-structural Segment 1 (NS1) transfected endothelial modification [PDF]

, 2012
Background: Human Parvovirus B19 (PVB19) has been associated with myocarditis putative due to endothelial infection. Whether PVB19 infects endothelial cells and causes a modification of endothelial function and inflammation and, thus, disturbance of ...
Seizer, Peter, Seizer Peter, Paul, Angela, Thomas Wurster, Botnar Rene M., Schönberger, Tanja, Stellos, Konstantinos L., Gawaz, Meinrad Paul, Tanja Schönberger, Gawaz Meinrad, Schuster Andreas, Gawaz, Meinrad, Botnar, Rene M.; id_orcid, Stellos, Konstantinos, Paul Angela, Boris Bigalke, Stellos Konstantinos, Pölzelbauer Catharina, Schönberger Tanja, Poelzelbauer, Catharina, Wurster, Thomas Heinrich, Angela Paul, Konstantinos Stellos, Meinrad Gawaz, Bigalke, Boris, Bigalke Boris, Botnar, Rene Michael, Rene M Botnar, Andreas Schuster, Wurster Thomas, Peter Seizer, Schuster, Andreas, Wurster, Thomas, Schoenberger, Tanja, Pölzelbauer, Catharina, Catharina Pölzelbauer   +35 more
core   +2 more sources

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

FMDV replicons encoding green fluorescent protein are replication competent [PDF]

, 2014
The study of replication of viruses that require high bio-secure facilities can be accomplished with less stringent containment using non-infectious 'replicon' systems. The FMDV replicon system (pT7rep) reported by Mclnerney et al. (2000) was modified by
Stonehouse, Nicola J., Tulloch, Fiona Ashley, Ryan, MD, Rowlands, DJ, Jackson, T, Pathania, Uday, Ryan, Martin D, Tuthill, Toby, Lamond, Angus I.; id_orcid, Rowlands, David J, Nicholson, J, Rowlands, David J., Tulloch, F, Luke, GA, Luke, Garry A., Nicholson, John, Lamond, Angus I., Luke, Garry A, Lamond, Angus I, Haas, Juergen, Ryan, Martin D., Tuthill, T, Hass, Juergen, Tulloch, Fiona, Luke, Garry Alec, Stonehouse, Nicola J, Jackson, Terry, Lamond, AI, Ryan, Martin Denis, Pathania, U, Haas, J, Pathania, Uday Singh, Stonehouse, NJ   +32 more
core   +1 more source

Fluorescent proteins such as eGFP lead to catalytic oxidative stress in cells

Redox Biology, 2017
Fluorescent proteins are an important tool that has become omnipresent in life sciences research. They are frequently used for localization of proteins and monitoring of cells [1,2].
Douglas Ganini, Fabian Leinisch, Ashutosh Kumar, JinJie Jiang, Erik J. Tokar, Christine C. Malone, Robert M. Petrovich, Ronald P. Mason   +7 more
doaj   +1 more source