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Red fluorescent proteins engineered from green fluorescent proteins. [PDF]
Proc Natl Acad Sci U S A, 2023 Fluorescent proteins (FPs) form a fluorophore through autocatalysis from three consecutive amino acid residues within a polypeptide chain. The two major groups, green FPs (GFPs) and red FPs (RFPs), have distinct fluorophore structures; RFPs have an extended π-conjugation system with an additional double bond.
Imamura H +4 more
europepmc +3 more sources
The use of thermostable fluorescent proteins for live imaging in Sulfolobus acidocaldarius [PDF]
Frontiers in MicrobiologyIntroductionAmong hyperthermophilic organisms, in vivo protein localization is challenging due to the high growth temperatures that can disrupt proper folding and function of mostly mesophilic-derived fluorescent proteins.
Alejandra Recalde +6 more
doaj +2 more sources
Very bright green fluorescent proteins from the Pontellid copepod Pontella mimocerami. [PDF]
PLoS ONE, 2010 Fluorescent proteins (FP) homologous to the green fluorescent protein (GFP) from the jellyfish Aequorea victoria have revolutionized biomedical research due to their usefulness as genetically encoded fluorescent labels. Fluorescent proteins from copepods
Marguerite E Hunt +3 more
doaj +3 more sources
Far-Red Fluorescent Proteins: Tools for Advancing In Vivo Imaging [PDF]
BiosensorsFar-red fluorescent proteins (FPs) have emerged as indispensable tools in in vivo imaging, playing a pivotal role in elucidating fundamental mechanisms and addressing application issues in biotechnology and biomedical fields.
Angyang Shang +3 more
doaj +2 more sources
Circularly Permuted Far-Red Fluorescent Proteins
Biosensors, 2021 The color palette of genetically encoded fluorescent protein indicators (GEFPIs) has expanded rapidly in recent years. GEFPIs with excitation and emission within the “optical window” above 600 nm are expected to be superior in many aspects, such as ...
Tianchen Wu, Yu Pang, Hui-wang Ai
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Tracking protein turnover and degradation by microscopy: photo-switchable versus time-encoded fluorescent proteins [PDF]
Open Biology, 2014 Expanded fluorescent protein techniques employing photo-switchable and fluorescent timer proteins have become important tools in biological research. These tools allow researchers to address a major challenge in cell and developmental biology, namely ...
Michael Knop, Bruce A. Edgar
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BioTechniques, 2023 Fluorescent proteins, such as green fluorescent proteins, are invaluable tools for detecting and quantifying gene expression in high-throughput reporter gene assays. However, they introduce significant inaccuracies in studies involving microaerobiosis or
Lucas Pedraz, Eduard Torrents
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Visible Fluorescent Proteins [PDF]
Journal of Biomedical Optics, 2008 This PDF file contains the editorial “Special Section Guest Editorial: Visible Fluorescent Proteins” for JBO Vol.
PERIASAMY A, DIASPRO, ALBERTO GIOVANNI
openaire +4 more sources
Fluorogen-activating proteins: beyond classical fluorescent proteins
Acta Pharmaceutica Sinica B, 2018 Fluorescence imaging is a powerful technique for the real-time noninvasive monitoring of protein dynamics. Recently, fluorogen activating proteins (FAPs)/fluorogen probes for protein imaging were developed.
Shengnan Xu, Hai-Yu Hu
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Fluorescence polarization of green fluorescence protein [PDF]
Proceedings of the National Academy of Sciences, 2002 We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea .
Shinya, Inoué +4 more
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