Results 11 to 20 of about 2,532,057 (298)

Diversity and evolution of coral fluorescent proteins. [PDF]

open access: yesPLoS ONE, 2008
GFP-like fluorescent proteins (FPs) are the key color determinants in reef-building corals (class Anthozoa, order Scleractinia) and are of considerable interest as potential genetically encoded fluorescent labels.
Naila O Alieva   +9 more
doaj   +2 more sources

Applications of Computational Protein Design to Red Fluorescent Proteins [PDF]

open access: yes, 2013
The research comprising this thesis is presented in three chapters divided into two parts. Part One, Chapters 2 and 3, of the thesis focuses on the application of rational design and CPD to the core residues of FPs.
Moore, Matthew Michaels
core   +1 more source

Modeling Spectral Tuning in Red Fluorescent Proteins Using the Dipole Moment Variation upon Excitation [PDF]

open access: yes, 2021
We describe a model for spectral tuning in red fluorescent proteins (RFPs) based on the relation between an electronic structure descriptor, the dipole moment variation upon excitation (DMV), and the excitation energy of a protein.
Maria, Khrenova   +2 more
core   +1 more source

Versatile toolbox for high throughput biochemical and functional studies with fluorescent fusion proteins [PDF]

open access: yes, 2012
Fluorescent fusion proteins are widely used to study protein localization and interaction dynamics in living cells. However, to fully characterize proteins and to understand their function it is crucial to determine biochemical characteristics such as ...
Sandra B. Hake (165089)   +15 more
core   +2 more sources

Expression of recombinant multi-coloured fluorescent antibodies in gor -/trxB- E. coli cytoplasm [PDF]

open access: yes, 2011
Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V.   +26 more
core   +1 more source

ATP changes the fluorescence lifetime of cyan fluorescent protein via an interaction with His148 [PDF]

open access: yes, 2010
Contains fulltext : 87235.pdf (Publisher’s version ) (Open Access)Recently, we described that ATP induces changes in YFP/CFP fluorescence intensities of Fluorescence Resonance Energy Transfer (FRET) sensors based on CFP-YFP.
Marieke Willemse   +28 more
core   +3 more sources

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs [PDF]

open access: yes, 2010
Förster resonance energy transfer (FRET) is a powerful method for obtaining information about small-scale lengths between biomacromolecules. Visible fluorescent proteins (VFPs) are widely used as spectrally different FRET pairs, where one VFP acts as a ...
Birch, D.J.S.   +8 more
core   +1 more source

Development of cysteine-free fluorescent proteins for the oxidative environment. [PDF]

open access: yesPLoS ONE, 2012
Molecular imaging employing fluorescent proteins has been widely used to highlight specific reactions or processes in various fields of the life sciences.
Takahisa Suzuki   +8 more
doaj   +1 more source

Bio-optimized energy transfer in densely packed fluorescent protein enables near-maximal luminescence and solid-state lasers [PDF]

open access: yes, 2014
This work was supported by the US National Science Foundation (ECCS- 1101947), National Institutes of Health (P41EB015903, R21EB013761), Department of Defense (FA9550-11-1-0331), and the Korea National Research Foundation (R31-2008-000-10071-0).
Gather, Malte Christian, Yun, Seok Hyun
core   +1 more source

Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae.

open access: yesPLoS ONE, 2013
Fluorescent protein fusions are a powerful tool to monitor the localization and trafficking of proteins. Such studies are particularly easy to carry out in the budding yeast Saccharomyces cerevisiae due to the ease with which tags can be introduced into ...
Sidae Lee   +3 more
doaj   +1 more source

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