Results 41 to 50 of about 2,532,057 (298)

Three-Color Imaging Using Fluorescent Proteins in Living Zebrafish Embryos

BioTechniques, 2001
The zebrafish embryo is especially valuable for cell biological studies because of its optical clarity. In this system, use of an in vivo fluorescent reporter has been limited to green fluorescent protein (GFP).
Kenneth R. Finley, Ann E. Davidson, Stephen C. Ekker   +2 more
doaj   +1 more source

Fluorescence color diversity of great barrier reef corals [PDF]

Journal of Innovative Optical Health Sciences, 2015
A group of variously colored proteins belonging to the green fluorescent protein (GFP) family are responsible for coloring coral tissues. Corals of the Great Barrier Reef were studied with the custom-built fiber laser fluorescence spectrometers. Spectral
Grigory Lapshin, Anya Salih, Peter Kolosov, Maria Golovkina, Yuri Zavorotnyi, Tatyana Ivashina, Leonid Vinokurov, Victor Bagratashvili, Alexander Savitsky   +8 more
doaj   +1 more source

Mutagenesis of mNeptune Red-Shifts Emission Spectrum to 681-685 nm.

PLoS ONE, 2016
GFP-like fluorescent proteins with diverse emission wavelengths have been developed through mutagenesis, offering many possible choices in cellular and tissue imaging, such as multi-targets imaging, deep tissue imaging that require longer emission ...
ZhaoYang Li, ZhiPing Zhang, LiJun Bi, ZongQiang Cui, JiaoYu Deng, DianBing Wang, Xian-En Zhang   +6 more
doaj   +1 more source

On the Origin of Fluorescence in Bacteriophytochrome Infrared Fluorescent Proteins [PDF]

The Journal of Physical Chemistry B, 2010
Tsien et al. (Science, 2009, 324, 804-807) recently reported the creation of the first infrared fluorescent protein (IFP). It was engineered from bacterial phytochrome by removing the PHY and histidine kinase-related domains, by optimizing the protein to prevent dimerization, and by limiting the biliverdins conformational freedom, especially around its
Alex A, Samma, Chelsea K, Johnson, Shuang, Song, Samuel, Alvarez, Marc, Zimmer   +4 more
openaire   +2 more sources

Electrochemical fluorescence switching of enhanced green fluorescent protein

Biosensors and Bioelectronics, 2023
Switchable fluorescent proteins, for which fluorescence can be switched ON and OFF, are widely used for molecule tracking and super resolution imaging. However, the robust use of the switchable fluorescent proteins is still limited as either the switching is not repeatable, or such switching requires irradiation with coupled lasers of different ...
Yang, Y, Fan, S, Webb, JA, Ma, Y, Goyette, J, Chen, X, Gaus, K, Tilley, RD, Gooding, JJ   +8 more
openaire   +3 more sources

Coalescence of the sites of cowpea mosaic virus RNA replication into a cytopathic structure [PDF]

, 2002
Cowpea mosaic virus (CPMV) replication induces an extensive proliferation of endoplasmic reticulum (ER) membranes, leading to the formation of small membranous vesicles where viral RNA replication takes place.
Carette, J.E., Gühl, K., Wellink, J., Kammen, A., van   +3 more
core   +1 more source

Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein [PDF]

Journal of the American Chemical Society, 2017
Green fluorescent protein, GFP, has revolutionized biology, due to its use in bioimaging. It is widely accepted that the protein environment makes its chromophore fluoresce, whereas the fluorescence is completely lost when the native chromophore is taken out of GFP.
Annette Svendsen, Hjalte V. Kiefer, Henrik B. Pedersen, Anastasia V. Bochenkova, Lars H. Andersen   +4 more
openaire   +3 more sources

Effect of DFPP on Ferroptosis in Peripheral Blood CD14+ Monocytes of Hyperlipidemic Patients Involving the ACSL4/LPCAT3/ALOX5 Pathway

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction Hyperlipidemia is a major risk factor for atherosclerotic cardiovascular disease (ASCVD). Although statins are the cornerstone of lipid‐lowering therapy, their use may be limited in some patients by adverse effects. This study evaluated the possible impact of non‐drug treatment using double‐filtration plasmapheresis (DFPP) on CD14+
Yun Cheng, Lan Li, Qingmei Yang, Meipeng Wu   +3 more
wiley   +1 more source

Fluorescent proteins as tools to aid protein production

Microbial Cell Factories, 2005
Fluorescent proteins are genetically encoded, highly versatile reporters useful for monitoring various aspects of recombinant protein production. In addition to the widely popular green fluorescent protein (GFP) from Aequorea victoria, a variety of other
Su Wei
doaj   +1 more source

Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]

The Journal of Physical Chemistry B, 1999
The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G., Subramaniam, V., Seidel, C., Volkmer, A.   +3 more
openaire   +2 more sources