Results 41 to 50 of about 761,199 (307)

Fluorescence color diversity of great barrier reef corals [PDF]

Journal of Innovative Optical Health Sciences, 2015
A group of variously colored proteins belonging to the green fluorescent protein (GFP) family are responsible for coloring coral tissues. Corals of the Great Barrier Reef were studied with the custom-built fiber laser fluorescence spectrometers. Spectral
Grigory Lapshin, Anya Salih, Peter Kolosov, Maria Golovkina, Yuri Zavorotnyi, Tatyana Ivashina, Leonid Vinokurov, Victor Bagratashvili, Alexander Savitsky   +8 more
doaj   +1 more source

Mutagenesis of mNeptune Red-Shifts Emission Spectrum to 681-685 nm.

PLoS ONE, 2016
GFP-like fluorescent proteins with diverse emission wavelengths have been developed through mutagenesis, offering many possible choices in cellular and tissue imaging, such as multi-targets imaging, deep tissue imaging that require longer emission ...
ZhaoYang Li, ZhiPing Zhang, LiJun Bi, ZongQiang Cui, JiaoYu Deng, DianBing Wang, Xian-En Zhang   +6 more
doaj   +1 more source

Three-Color Imaging Using Fluorescent Proteins in Living Zebrafish Embryos

BioTechniques, 2001
The zebrafish embryo is especially valuable for cell biological studies because of its optical clarity. In this system, use of an in vivo fluorescent reporter has been limited to green fluorescent protein (GFP).
Kenneth R. Finley, Ann E. Davidson, Stephen C. Ekker   +2 more
doaj   +1 more source

PNA-induced assembly of fluorescent proteins using DNA as a framework

, 2013
Controlled alignment of proteins on molecular frameworks requires the development of facile and orthogonal chemical approaches and molecular scaffolds. In this work, protein−PNA conjugates are brought forward as new chemical components allowing efficient
Gholami, Z., Brunsveld, L., Gholami, Z, Hanley, Q, Hanley, Q., Brunsweld, L, Brunsveld, L.; id_orcid   +6 more
core   +1 more source

Mapping of tonoplast intrinsic proteins in maturing and germinating Arabidopsis seeds reveals dual localization of embryonic TIPs to the tonoplast and plasma membrane [PDF]

, 2011
We have mapped the expression of the tonoplast intrinsic protein (TIP) gene family members in Arabidopsis seeds by fluorescent protein tagging of their genomic sequences and confocal microscopy.
Mathias Sorieul, Stefano Gattolin, Gattolin, Stefano, Frigerio, Lorenzo, Sorieul, Mathias, Lorenzo Frigerio   +5 more
core   +1 more source

Circular permutation of red fluorescent proteins.

PLoS ONE, 2011
Circular permutation of fluorescent proteins provides a substrate for the design of molecular sensors. Here we describe a systematic exploration of permutation sites for mCherry and mKate using a tandem fusion template approach.
Bo Shui, Qi Wang, Frank Lee, Laura J Byrnes, Dmitry M Chudakov, Sergey A Lukyanov, Holger Sondermann, Michael I Kotlikoff   +7 more
doaj   +1 more source

Genetically Encoded Biosensors Based on Fluorescent Proteins

Sensors, 2021
Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes.
Hyunbin Kim, Jeongmin Ju, Hae Nim Lee, Hyeyeon Chun, Jihye Seong   +4 more
doaj   +1 more source

Fluorescent proteins as tools to aid protein production

Microbial Cell Factories, 2005
Fluorescent proteins are genetically encoded, highly versatile reporters useful for monitoring various aspects of recombinant protein production. In addition to the widely popular green fluorescent protein (GFP) from Aequorea victoria, a variety of other
Su Wei
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs

, 2010
Förster resonance energy transfer (FRET) is a powerful method for obtaining information about small-scale lengths between biomacromolecules. Visible fluorescent proteins (VFPs) are widely used as spectrally different FRET pairs, where one VFP acts as a ...
Birch, D.J.S., Laptenok, S., Visser, N.V., Hoek, A., van, Laptenok, S.P., Borst, J.W., Visser, A.J.W.G., van Hoek, A., Brochon, J.C.   +8 more
core   +1 more source