Results 51 to 60 of about 2,532,057 (298)
Corrole-Substituted Fluorescent Heme Proteins [PDF]
, 2021 Although fluorescent proteins have been utilized for a variety of biological applications, they have several optical limitations, namely weak red and near-infrared emission and exceptionally broad (>200 nm) emission profiles.
Marletta, Michael A +1 more
core +1 more source
FEBS Letters, EarlyView.Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi +5 more
wiley +1 more source
Development of destabilized mCherry fluorescent proteins for applications in the model yeast
Biotechnology NotesFluorescent proteins are widely used molecular reporters in studying gene expression and subcellular protein localization. To enable the monitoring of transient cellular events in the model yeast Saccharomyces cerevisiae , destabilized green and cyan ...
Yu Chyuan Heng, Jee Loon Foo
doaj +1 more source
Circular permutation of red fluorescent proteins.
PLoS ONE, 2011 Circular permutation of fluorescent proteins provides a substrate for the design of molecular sensors. Here we describe a systematic exploration of permutation sites for mCherry and mKate using a tandem fusion template approach.
Bo Shui +7 more
doaj +1 more source
Modern fluorescent techniques to investigate the mechanisms of lymphocyte activation [PDF]
The Ukrainian Biochemical Journal, 2015 Fluorescent proteins are promising tools for studying intracellular signaling processes in lymphocytes. This brief review summarizes fluorescence-based imaging techniques developed in recent years and discusses new methodological advances, such as ...
G. A. Liubchenko +2 more
doaj +1 more source
Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source
Ataxin-1 fusion partners alter polyQ lethality and aggregation [PDF]
, 2007 Intranuclear inclusion bodies (IBs) are the histopathologic markers of multiple protein folding diseases. IB formation has been extensively studied using fluorescent fusion products of pathogenic polyglutamine (polyQ) expressing proteins.
Rich, T. +5 more
core +2 more sources
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins. [PDF]
FEBS Open BioEndogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Ikeda K.
europepmc +2 more sources
Green fluorescent protein-based monitoring of endoplasmic reticulum redox poise [PDF]
, 2013 Pathological endoplasmic reticulum (ER) stress is tightly linked to the accumulation of reactive oxidants, which can be both upstream and downstream of ER stress.
Julia Birk +11 more
core +1 more source