Results 61 to 70 of about 761,199 (307)

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Express your LOV: an engineered flavoprotein as a reporter for protein expression and purification [PDF]

, 2012
In this work, we describe the utility of Light, Oxygen, or Voltage-sensing (LOV) flavoprotein domains from plant phototropins as a reporter for protein expression and function.
Jayde A Gawthorne, Akpunarlieva, S.N., John M. Christie (107410), Beckham, K.S.H., Gabrielsen, M., Katherine S. H. Beckham (107407), Alto, Neal M., Alto, N.M., Christie, J.M., Snezhana N. Akpunarlieva (107403), Jayde A. Gawthorne (107393), Mads Gabrielsen (107416), Reddick, L.E., Akpunarlieva, Snezhana N., Jayde A. Gawthorne, Roe, A.J., Katherine S H Beckham, Christie, John M., Snezhana N. Akpunarlieva, L. Evan Reddick (107398), Beckham, Katherine S. H., Mads Gabrielsen, Andrew J Roe, John M Christie, Gabrielsen, Mads, Gawthorne, J.A., Reddick, L. Evan, Andrew J. Roe, Neal M. Alto (107413), John M. Christie, L. Evan Reddick, Snezhana N Akpunarlieva, Neal M. Alto, Andrew J. Roe (107419), Neal M Alto, Roe, Andrew J., Katherine S. H. Beckham, L Evan Reddick, Gawthorne, Jayde A.   +38 more
core   +1 more source

Monomeric red fluorescent proteins [PDF]

, 2023
Disclosed are sequences encoding monomeric variants of DsRed fluorescent proteins and methods of ...

core   +4 more sources

Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment.

PLoS ONE, 2015
We report the identification and characterization of two new members of a family of bilirubin-inducible fluorescent proteins (FPs) from marine chlopsid eels and demonstrate a key region of the sequence that serves as an evolutionary switch from non ...
David F Gruber, Jean P Gaffney, Shaadi Mehr, Rob DeSalle, John S Sparks, Jelena Platisa, Vincent A Pieribone   +6 more
doaj   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

6 fluorescent proteins unmixed

, 2021
6 fluorescent proteins ...
Simon Monard (11415095)
core   +1 more source

Modulation of Fluorescent Protein Chromophores To Detect Protein Aggregation with Turn-On Fluorescence [PDF]

Journal of the American Chemical Society, 2018
We present a fluorogenic method to visualize misfolding and aggregation of a specific protein-of-interest in live cells using structurally modulated fluorescent protein chromophores. Combining photophysical analysis, X-ray crystallography, and theoretical calculation, we show that fluorescence is triggered by inhibition of twisted-intramolecular charge
Yu Liu, Charles H. Wolstenholme, Gregory C. Carter, Hongbin Liu, Hang Hu, Leeann S. Grainger, Kun Miao, Matthew Fares, Conner A. Hoelzel, Hemant P. Yennawar, Gang Ning, Manyu Du, Lu Bai, Xiaosong Li, Xin Zhang   +14 more
openaire   +2 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Visualization of protein-protein interactions in the secretory pathway of mammalian cells [PDF]

, 2006
An increasing number of human disorders are being linked to mutations in components of the secretory pathway. One example is combined blood coagulation factor V and VIII deficiency, an autosomal recessive disorder leading to hemophilia due to markedly ...
Nyfeler, Beat
core   +1 more source

Efficient Co-Replication of Defective Novirhabdovirus

Viruses, 2016
We have generated defective Viral Hemorrhagic Septicemia Viruses (VHSV) which express either the green fluorescent protein (GFP) or a far-red fluorescent protein (mKate) by replacing the genes encoding the nucleoprotein N or the polymerase-associated P ...
Ronan N. Rouxel, Emilie Mérour, Stéphane Biacchesi, Michel Brémont   +3 more
doaj   +1 more source