Results 61 to 70 of about 2,532,057 (298)

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Novel fluorescent probes for analysis of protein interactions under truly physiological conditions with real medical devices [PDF]

, 2012
PhDProtein adsorption under physiological conditions was recognised to be the key step in the modulation of biological responses between materials and an osseous environment.
Mafina, Marc-Krystelle
core   +4 more sources

An Endogenously Tagged Fluorescent Fusion Protein Library in Mouse Embryonic Stem Cells

Stem Cell Reports, 2017
Embryonic stem cells (ESCs), with their dual capacity to self-renew and differentiate, are commonly used to study differentiation, epigenetic regulation, lineage choices, and more. Using non-directed retroviral integration of a YFP/Cherry exon into mouse
Arigela Harikumar, Raghu Ram Edupuganti, Matan Sorek, Gajendra Kumar Azad, Styliani Markoulaki, Petra Sehnalová, Soňa Legartová, Eva Bártová, Shlomit Farkash-Amar, Rudolf Jaenisch, Uri Alon, Eran Meshorer   +11 more
doaj   +1 more source

Quantitative assessment of fluorescent proteins [PDF]

Nature Methods, 2016
The advent of fluorescent proteins (FPs) for genetic labeling of molecules and cells has revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of bright and stable FPs spanning blue to red spectral regions. Common to autofluorescent FPs is their tight β-barrel structure, which provides the rigidity and chemical ...
Cranfill, P J, Sell, B R, Baird, M A, Allen, J R, Lavagnino, Z, de Gruiter, Martijn, Kremers, Gert-Jan, Davidson, M W, Ustione, A, Piston, D W   +9 more
openaire   +2 more sources

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Express your LOV: an engineered flavoprotein as a reporter for protein expression and purification [PDF]

, 2012
In this work, we describe the utility of Light, Oxygen, or Voltage-sensing (LOV) flavoprotein domains from plant phototropins as a reporter for protein expression and function.
Jayde A Gawthorne, Akpunarlieva, S.N., John M. Christie (107410), Beckham, K.S.H., Gabrielsen, M., Katherine S. H. Beckham (107407), Alto, Neal M., Alto, N.M., Christie, J.M., Snezhana N. Akpunarlieva (107403), Jayde A. Gawthorne (107393), Mads Gabrielsen (107416), Reddick, L.E., Akpunarlieva, Snezhana N., Jayde A. Gawthorne, Roe, A.J., Katherine S H Beckham, Christie, John M., Snezhana N. Akpunarlieva, L. Evan Reddick (107398), Beckham, Katherine S. H., Mads Gabrielsen, Andrew J Roe, John M Christie, Gabrielsen, Mads, Gawthorne, J.A., Reddick, L. Evan, Andrew J. Roe, Neal M. Alto (107413), John M. Christie, L. Evan Reddick, Snezhana N Akpunarlieva, Neal M. Alto, Andrew J. Roe (107419), Neal M Alto, Roe, Andrew J., Katherine S. H. Beckham, L Evan Reddick, Gawthorne, Jayde A.   +38 more
core   +2 more sources

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment.

PLoS ONE, 2015
We report the identification and characterization of two new members of a family of bilirubin-inducible fluorescent proteins (FPs) from marine chlopsid eels and demonstrate a key region of the sequence that serves as an evolutionary switch from non ...
David F Gruber, Jean P Gaffney, Shaadi Mehr, Rob DeSalle, John S Sparks, Jelena Platisa, Vincent A Pieribone   +6 more
doaj   +1 more source

Fluorescent glycan fingerprinting of SARS2 spike proteins

Scientific Reports, 2021
Glycosylation is the most common post-translational modification and has myriad of biological functions. However, glycan analysis has always been a challenge.
Zhengliang L. Wu, James M. Ertelt
doaj   +1 more source

Genetically Encoded Biosensors Based on Fluorescent Proteins

Sensors, 2021
Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes.
Hyunbin Kim, Jeongmin Ju, Hae Nim Lee, Hyeyeon Chun, Jihye Seong   +4 more
doaj   +1 more source