Results 1 to 10 of about 435,897 (168)

The significance of C-terminal NLS sequences of VirD2 in its nuclear localization in Saccharomyces cerevisiae [PDF]

open access: yesJournal of Cell and Molecular Research, 2013
Agrobacterium tumefaciens is capable of gene transfer to both plant and non-plant organisms. Indeed, upon infection of eukaryotic cells, Agrobacterium tumefaciens transfers a piece of its tumor inducing (Ti)-plasmid, called T-DNA, to the host cell ...
Jalal Soltani   +3 more
doaj   +4 more sources

Molecular and electrophysiological characterization of GFP-expressing CA1 interneurons in GAD65-GFP mice. [PDF]

open access: yesPLoS ONE, 2010
The use of transgenic mice in which subtypes of neurons are labeled with a fluorescent protein has greatly facilitated modern neuroscience research. GAD65-GFP mice, which have GABAergic interneurons labeled with GFP, are widely used in many research ...
Corette J Wierenga   +5 more
doaj   +5 more sources

Peculiarities of green fluorescent protein transgene detection in tobacco and maize plants by PCR [PDF]

open access: yesBiotechnologia Acta, 2023
The aim of the work was to investigate detection of different modifications of the green fluorescent protein gene (gfp) in the transgenic tobacco and maize plants by polymerase chain reaction (PCR). Methods.
Nitovska І. О.   +2 more
doaj   +1 more source

Optimization of transient gene expression in tomato (Solanum lycopersicum L.) [PDF]

open access: yesمجله بیوتکنولوژی کشاورزی, 2023
ObjectiveThe aim of this study is to optimize tomato as a suitable plant system for transient gene transformation for the production of pharmaceutical and industrial compounds.Materials and methodsIn order to investigate transient gene expression, three ...
Masomeh Nasiri Mogadam   +3 more
doaj   +1 more source

Downregulation of FeSOD-A expression in Leishmania infantum alters trivalent antimony and miltefosine susceptibility

open access: yesParasites & Vectors, 2021
Background Superoxide dismutase (SOD), a central component of the antioxidant defence system of most organisms, removes excess superoxide anions by converting them to oxygen and hydrogen peroxide. As iron (Fe) SOD is absent in the human host, this enzyme
Ana Maria Murta Santi   +3 more
doaj   +1 more source

UV Light Induced Fluorescence Recovery of GFP After Photobleaching in Microscopy Imaging

open access: yesImage Analysis and Stereology, 2022
Fluorescence microscopy has become one of the most important tools for biologists to visualize and study organelles and molecules in a cell. Fluorescent markers are used to visualize specific molecules.
Griša G. Prinčič   +4 more
doaj   +1 more source

Lentiviral vector-mediated transduction of adult neural stem/progenitor cells isolated from the temporal tissues of epileptic patients [PDF]

open access: yesIranian Journal of Basic Medical Sciences, 2020
Objective(s): Neural stem/progenitor cells (NS/PCs) hold a great potential for delivery of therapeutic agents into the injured regions of the brain.
Sara Abdollahi   +6 more
doaj   +1 more source

Using Scuba for In Situ Determination of Chlorophyll Distributions in Corals by Underwater Near Infrared Fluorescence Imaging

open access: yesJournal of Marine Science and Engineering, 2020
Studies reporting quantitation and imaging of chlorophyll in corals using visible fluorescent emission in the red near 680 nm can suffer from competing emission from other red-emitting pigments.
Thomas Oh   +2 more
doaj   +1 more source

Trypanin Disruption Affects the Motility and Infectivity of the Protozoan Trypanosoma cruzi

open access: yesFrontiers in Cellular and Infection Microbiology, 2022
The flagellum of Trypanosomatids is an organelle that contributes to multiple functions, including motility, cell division, and host–pathogen interaction.
Jose L. Saenz-Garcia   +10 more
doaj   +1 more source

Production of hGCSF and GFP Co-Expressed Transgenic Cow Embryo by Somatic Cell Nuclear Transfer Technique

open access: yesJournal of Animal Reproduction and Biotechnology, 2015
The purpose of this study is to develop transgenic cell line expressing targeted human granulocyte colony stimulating factor (hGCSF) and green fluorescence protein (GFP) genes as well as production of Somatic Cell Nuclear Transfer (SCNT) embryos derived ...
Jung Seok Yang   +8 more
doaj   +1 more source

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