Results 1 to 10 of about 520,090 (317)

Applications of Green Fluorescent Protein in Plants

open access: yesBioTechniques, 1997
Green fluorescent protein (GFP) is increasingly being used in plant biology from the cellular level to whole plant level. At the cellular level, GFP is being used as an in vivo reporter to assess frequency of transient and stable transformation.
Staci M. Leffel   +2 more
doaj   +1 more source

Pan02 pancreatic tumor models carrying the GFP marker in mice

open access: yesМедицинская иммунология
Animal tumor models are used for preclinical studies of drugs and cancer therapy. The aim of this work was to analyze the growth of murine pancreatic tumor cells Pan02, carrying GFP marker, injected subcutaneously (s. c.), intraperitoneally (i.
S. B. Akopov   +5 more
doaj   +1 more source

The role of explant type and selective agent application in the initial transformation rate of Lens culinaris Medik.

open access: yesВавиловский журнал генетики и селекции
Lens culinaris Medik. (lentil) is an agronomically important leguminous species, but its genome modification is rarely used for obtaining new varieties, probably due to a low efficiency of transformation protocols.
T. V. Dyubenko   +2 more
doaj   +1 more source

For the last time, it is GFP-Atg8, not Atg8-GFP (and the same goes for LC3) [PDF]

open access: yesAutophagy, 2011
GFP-Atg8/LC3 is a very useful marker to follow macroautophagy (hereafter autophagy). In contrast, Atg8/LC3-GFP has relatively limited uses. Unfortunately, many authors do not seem to appreciate the difference between these two constructs. I hope to put an end to that now.
openaire   +2 more sources

RECONSTRUCTION OF MECHANISMS REGULATING THE EXPRESSION OF THE ESCHERICHIA COLI YFIA GENE UNDER STRESS CONDITIONS

open access: yesВавиловский журнал генетики и селекции, 2014
The regulatory region of the Escherichia coli yfiA gene was reconstructed by using the SiteCon web resource and mathematical modeling, and its expression complexity under oxidative stress was assessed. Simulation of the response of E.
T. M. Khlebodarova   +5 more
doaj  

Kharon Is Crucial for Trypanosoma cruzi Morphology but Does Not Impair In Vitro Infection

open access: yesPathogens
Chagas disease, caused by Trypanosoma cruzi, is a neglected tropical disease with few options for treatment and no available vaccine. Deletion mutants for live attenuated vaccines, particularly deletions of proteins related to the cytoskeleton, have been
Jose Luis Saenz-Garcia   +9 more
doaj   +1 more source

Lack of Evidence for Recipient Precursor Cells Replenishing β-Cells in Transplanted Islets

open access: yesCell Transplantation, 2010
Bone marrow and tissue precursor cells have been postulated to replenish grafts of transplanted islets. Several investigators have reported that bone marrow cells can promote the regeneration of injured islets.
Yoshiyuki Hamamoto   +4 more
doaj   +1 more source

Evaluation of different promoters driving the GFP reporter gene in seaweed Kappaphycus alvarezii

open access: yesIndonesian Journal of Biotechnology, 2014
Promoter regulates expression level of foreign gene in transgenic organism. This study was performed to select a suitable promoter as the fi rst step towards production of valuable trait-enhanced seaweed by transgenic technology.
Muh. Alias L. Rajamuddin   +3 more
doaj   +1 more source

Efficient Gene Transfer and Expression in Islets by an Adenoviral Vector that Lacks All Viral Genes

open access: yesCell Transplantation, 1999
Although adenoviral vector-mediated gene transfer has significant potential for gene therapy, host immune responses to virally expressed proteins and small insert capacity may limit its clinical application.
Zhiguang Guo   +7 more
doaj   +1 more source

Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed mice

open access: yesnpj Science of Food
Polysaccharide extracted from Grifola frondosa (GFP) was selected in this study. After preliminary separation, four factions were collected, named GFP-F1, GFP-F2, GFP-F3 and GFP-F4.
Yin-Yi Ding   +7 more
doaj   +1 more source

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