Results 31 to 40 of about 537,728 (218)

Aristaless-related homeobox plays a key role in hyperplasia of the pancreas islet α-like cells in mice deficient in proglucagon-derived peptides. [PDF]

PLoS ONE, 2013
Defects in glucagon action can cause hyperplasia of islet α-cells, however, the underlying mechanisms remain largely to be elucidated. Mice homozygous for a glucagon-GFP knock-in allele (Gcg(gfp/gfp) ) completely lack proglucagon-derived peptides and ...
Sai Xu, Yoshitaka Hayashi, Yoshiko Takagishi, Mariko Itoh, Yoshiharu Murata   +4 more
doaj   +1 more source

GFP-Atg8 localization by fluorescence microscopy.

, 2020
KN99α+GFP-Atg8 and atg4+GFP-Atg8 strains were cultured in YPD and SD/-N/-AA at 30°C and 37°C for 4 h. Vacuole labeled with FM4-64 (Invitrogen) dye. (A) GFP-Atg8 localization during autophagy pathway. White arrows indicate the vacuole membrane. Scale bars
Renata Castiglioni Pascon (6612353), Thiago Nunes Roberto (8677083), Marcelo Afonso Vallim (5957576), Ricardo Ferreira Lima (8677086), Alexander Idnurm (58590)   +4 more
core   +1 more source

FRET Microscopy in Yeast

Biosensors, 2019
Förster resonance energy transfer (FRET) microscopy is a powerful fluorescence microscopy method to study the nanoscale organization of multiprotein assemblies in vivo.
Michal Skruzny, Emma Pohl, Marc Abella
doaj   +1 more source

Training GFP to fold [PDF]

Nature Methods, 2006
A method to select for proteins that are highly efficient at folding yields 'superfolder' GFP, an optimized GFP for use in protein fusions.
openaire   +2 more sources

Examples of genes from PANTHER protein classes “Transcriptional Regulation”, “Defense/Immunity”, “Transporters”, and “Transmembrane Signal Receptors” that were significantly differentially expressed in DND1-GFP-lo compared to DND1-GFP-hi cells at E16.5 and/or E18.5.

, 2023
hiloRNA-seq expression P-value (DESeq2) between DND1-GFP-lo and DND1-GFP-hi cells at E16.5 and E18.5: not significant (ns), (TIF)
Jack D. Keene (14701357), Blanche Capel (16995), Victor A. Ruthig (14701345), Josiah Hardy (14701348), Talia Hatkevich (6639401), Matthew B. Friedersdorf (14701351), Chloé Mayère (14701354), Serge Nef (45552)   +7 more
core   +1 more source

Presentation of antigen on extracellular vesicles using transmembrane domains from viral glycoproteins for enhanced immunogenicity

Journal of Extracellular Vesicles, 2022
A vaccine antigen, when launched as DNA or RNA, can be presented in various forms, including intracellular, secreted, membrane‐bound, or on extracellular vesicles (EVs).
Kai Hu, Paul F. McKay, Karnyart Samnuan, Adrian Najer, Anna K. Blakney, Junyi Che, Gwen O'Driscoll, Martina Cihova, Molly M. Stevens, Robin J. Shattock   +9 more
doaj   +1 more source

Preliminary activity (see Fig 1A): Characterization of the pCMV3-GFP-Spike plasmid and the RNA produced by untreated A549 cells and A549 cells transfected with the pCMV3-GFP-Spike plasmid.

, 2022
A. 0.8% w/v agarose gel electrophoresis of the pCMV3-GFP-Spike plasmid. B. 0.8% w/v agarose gel electrophoresis of RNA isolated from untreated A549 cells or cells transfected with the pCMV3-GFP-Spike plasmid. C. Check on 1.8% w/v agarose gel of amplicons
Jessica Gasparello (2633761), Cristina Zuccato (717940), Matteo Zurlo (7372766), Giulia Breveglieri (717941), Lucia Carmela Cosenza (3779479), Roberto Gambari (328450), Alessia Finotti (717938), Chiara Papi (3013806)   +7 more
core   +1 more source

Stochastic activation of the response regulator PhoB by noncognate histidine kinases

Journal of Integrative Bioinformatics, 2005
Two-component systems (TCS) are the most prevalent gene regulatory mechanism in bacteria. A typical TCS is comprised of a histidine kinase (HK) and a partner response regulator (RR).
Zhou Lu, Grégori Gérald, Blackman Jennifer Masella, Robinson J. Paul, Wanner Barry L.   +4 more
doaj   +1 more source

GFP's Mechanical Intermediate States

PLoS ONE, 2012
PLoS ONE, 7 (10)
Saeger, John, Hytönen, Vesa P, Klotzsch, Enrico, Vogel, Viola   +3 more
openaire   +7 more sources

Ascorbate peroxidase modulation confirms key role in Leishmania infantum oxidative defence

Parasites & Vectors
Background Ascorbate peroxidase (APX) has emerged as a promising target for chemotherapy because of its absence in humans and crucial role in the antioxidant defence of trypanosomatids.
Isabella Fernandes Martins Santos, Douglas de Souza Moreira, Karla Ferreira Costa, Juliana Martins Ribeiro, Silvane Maria Fonseca Murta, Ana Maria Murta Santi   +5 more
doaj   +1 more source