Results 51 to 60 of about 520,090 (317)
Ascorbate peroxidase modulation confirms key role in Leishmania infantum oxidative defence
Parasites & VectorsBackground Ascorbate peroxidase (APX) has emerged as a promising target for chemotherapy because of its absence in humans and crucial role in the antioxidant defence of trypanosomatids.
Isabella Fernandes Martins Santos +5 more
doaj +1 more source
Biologia Plantarum, 2014 Fluorophore tagged proteins are used in Arabidopsis thaliana to understand their functional role in plant development. This requires the analysis of their spatial localization in planta.
F. M. Perrine-Walker, E. Jublanc
doaj +1 more source
GFP's Mechanical Intermediate States
PLoS ONE, 2012 PLoS ONE, 7 (10)
Saeger, John +3 more
openaire +7 more sources
FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
FEBS Letters, EarlyView.Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes +4 more
wiley +1 more source
, 2023 Complete list of apoptosis and cell death terms, with gene Ensembl IDs from Biological Process Gene Ontology analysis in g:Profiler of E16.5 DND1-GFP-hi and DND1-GFP-lo cells.
Jack D. Keene (14701357) +7 more
core +1 more source
Use of the Nanofitin Alternative Scaffold as a GFP-Ready Fusion Tag. [PDF]
PLoS ONE, 2015 With the continuous diversification of recombinant DNA technologies, the possibilities for new tailor-made protein engineering have extended on an on-going basis.
Simon Huet +4 more
doaj +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
GFP-Vps32 and GFP-Atg8 are recruited to the disrupted MCV at spatially distinct sites.
, 2019 (A) D. discoideum expressing GFP-Vps32 were infected with M. marinum (blue) and fixed for immunostaining against p80 to label the MCV (red). At 1.5 hpi, no GFP-Vps32 structure was visible at apparent intact MCVs.
Thierry Soldati (79025) +7 more
core +1 more source