Results 61 to 70 of about 520,090 (317)
Microbial Cell Factories, 2020 Background Inclusion bodies (IBs) are biologically active protein aggregates forming natural nanoparticles with a high stability and a slow-release behavior.
Ramon Roca-Pinilla +6 more
doaj +1 more source
Mice Deficient in Proglucagon-Derived Peptides Exhibit Glucose Intolerance on a High-Fat Diet but Are Resistant to Obesity. [PDF]
PLoS ONE, 2015 Homozygous glucagon-GFP knock-in mice (Gcggfp/gfp) lack proglucagon derived-peptides including glucagon and GLP-1, and are normoglycemic. We have previously shown that Gcggfp/gfp show improved glucose tolerance with enhanced insulin secretion.
Yusuke Takagi +6 more
doaj +1 more source
Biochemistry and Biophysics Reports, 2021 The widespread application of protein and peptide therapeutics is hampered by their poor stability, strong immunogenicity and short half-life. However, the existing protein modification technologies require the introduction of exogenous macromolecules ...
Jin Hu +5 more
doaj +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
Immunocytochemistry for SOX2 on GFP- and null-GFP-clusters after 2D-cultivation.
, 2018 (A, B): Immunostaining for SOX2 on GFP- (A) and null-GFP-clusters (B) after 2D-cultivation in GD-medium for 0, 4, and 7 days. SOX2 visualized with Cy3 (red), merged image with phase-contrast, and nuclear staining by DAPI (blue) are shown in upper, middle,
Masaaki Kobayashi (5130710) +8 more
core +1 more source
GFP-Osy localizes to pore canals.
, 2020 By confocal microscopy, in live L3 larvae, GFP-Osy (green) is detected at the surface of epidermal cells marked by ridges in the top view (A). As described recently, these ridges are produced by the basal site of the cuticle engraved into the apical ...
Nicole Gehring (3920018) +10 more
core +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Dynamics of Dgat2-GFP-LDs during infection.
, 2017 A. Dgat2-GFP-positive LDs attach to the bacteria when they are exposed to the cytosol (3 and 21 hpi). At a later infection stage, Dgat2-GFP completely surrounds a cytosolic bacterium (45 hpi).
Thierry Soldati (79025) +1 more
core +1 more source
The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes
FEBS Letters, EarlyView.Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga +3 more
wiley +1 more source