Results 61 to 70 of about 537,728 (218)

Tools for retargeting proteins within Aspergillus nidulans. [PDF]

PLoS ONE, 2017
Endogenously tagging proteins with green fluorescent protein (GFP) enables the visualization of the tagged protein using live cell microscopy. GFP-tagging is widely utilized to study biological processes in model experimental organisms including ...
Subbulakshmi Suresh, Leymaan Abdurehman, Aysha H Osmani, Stephen A Osmani   +3 more
doaj   +1 more source

Emergence of GFP-expressing PCs in SCA1 mouse cerebella after transplantation of TRE-GFP-hfMSCs expressing GFP through the Tet-off system.

, 2016
(a) The TRE-GFP gene was introduced into hfMSCs through a lentivirus (1). The transduced TRE-GFP-hfMSCs were injected into the cerebella of 6–8-month-old SCA1 mice (2).
Nobutaka Takahashi (1930330), Fathul Huda (3325365), Yasunori Matsuzaki (115418), Ayumu Konno (3091014), Yiping Fan (47091), Hirokazu Hirai (115426), Mamiko Suzuki (225727), Jerry K. Y. Chan (358275)   +7 more
core   +1 more source

Purification of a synthetic GFP-GFP-mCherry-Fc cytokine and analysis of signal transduction induced through GFP_VHH-gp130 and mCherry_VHH-gp130 SyCyRs.

, 2020
(A) Schematic illustration of a GFP-GFP-mCherry fusion protein covalently linked to the Fc domain of an IgG antibody. The Fc domain forms a dimer and this leads to a hexameric GFP-GFP-mCherry assembly with two mCherry proteins in the center and two GFP ...
Hoang T. Phan (8655357), Jens M. Moll (8655363), Jürgen Scheller (53995), Udo Conrad (575723), Sofie Mossner (8655354), Saskia Triller (8655360)   +5 more
core   +1 more source

GFP-CD9 expression in stable cell line.

, 2022
Cells from the GFP-CD9+ cell line were stained with Hoechst 33342 1:2,000 (in blue). Images were taken with a 40X objective. Expression of the fusion protein can be seen located to the cell membrane. (TIF)
Cristina Bellotti (12225254), Robert Steinfeld (7524614), Andreas Stäuble (12225257)   +2 more
core   +1 more source

RECONSTRUCTION OF MECHANISMS REGULATING THE EXPRESSION OF THE ESCHERICHIA COLI YFIA GENE UNDER STRESS CONDITIONS

Вавиловский журнал генетики и селекции, 2014
The regulatory region of the Escherichia coli yfiA gene was reconstructed by using the SiteCon web resource and mathematical modeling, and its expression complexity under oxidative stress was assessed. Simulation of the response of E.
T. M. Khlebodarova, D. Yu. Oshchepkov, N. V. Tikunova, I. V. Babkin, A. D. Gruzdev, V. A. Likhoshvai   +5 more
doaj  

Intercellular spread of GFP-VP22 [PDF]

The Journal of Gene Medicine, 1999
The herpes simplex virus type 1 (HSV-1) VP22 polypeptide has been reported to mediate intercellular trafficking of heterologous proteins fused to its C- or N-terminus, a feature making it a useful tool in bystander cell-targeted gene therapy.Here we show, by detection of Green Fluorescent Protein (GFP) fused to VP22, its subcellular distribution in ...
A, Aints, M S, Dilber, C I, Smith
openaire   +2 more sources

Characterisation of alternative expression vectors for recombinant Bacillus Calmette-Guérin as live bacterial delivery systems

Memorias do Instituto Oswaldo Cruz
BACKGROUND Bacillus Calmette-Guérin (BCG) is considered a promising live bacterial delivery system. However, several proposals for rBCG vaccines have not progressed, mainly due to the limitations of the available expression systems.
Larissa V Nascimento, Carina C Santos, Luciana CC Leite, Ivan P Nascimento   +3 more
doaj   +1 more source

Disappearance of GFP-positive hepatocytes transplanted into the liver of syngeneic wild-type rats pretreated with retrorsine.

PLoS ONE, 2014
Background and aimGreen fluorescent protein (GFP) is a widely used molecular tag to trace transplanted cells in rodent liver injury models. The differing results from various previously reported studies using GFP could be attributed to the immunogenicity
Hiromichi Maeda, Masatoshi Shigoka, Yongchun Wang, Yingxin Fu, Russell N Wesson, Qing Lin, Robert A Montgomery, Hideaki Enzan, Zhaoli Sun   +8 more
doaj   +1 more source

Bacterial Biosensors for Measuring Availability of Environmental Pollutants

Sensors, 2008
Traditionally, pollution risk assessment is based on the measurement of a pollutant’s total concentration in a sample. The toxicity of a given pollutant in the environment, however, is tightly linked to its bioavailability, which may differ ...
Jan Roelof van der Meer, Robin Tecon
doaj  

Applications of Green Fluorescent Protein in Plants

BioTechniques, 1997
Green fluorescent protein (GFP) is increasingly being used in plant biology from the cellular level to whole plant level. At the cellular level, GFP is being used as an in vivo reporter to assess frequency of transient and stable transformation.
Staci M. Leffel, Stephen A. Mabon, C. Neal Stewart   +2 more
doaj   +1 more source