Results 71 to 80 of about 701,605 (308)
FEBS Letters, EarlyView.Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley +1 more source
Nature Methods, 2006 A method to select for proteins that are highly efficient at folding yields 'superfolder' GFP, an optimized GFP for use in protein fusions.
openaire +2 more sources
AAA+ protein unfoldases—the Moirai of the proteome
FEBS Letters, EarlyView.AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley +1 more source
Tools for retargeting proteins within Aspergillus nidulans. [PDF]
PLoS ONE, 2017 Endogenously tagging proteins with green fluorescent protein (GFP) enables the visualization of the tagged protein using live cell microscopy. GFP-tagging is widely utilized to study biological processes in model experimental organisms including ...
Subbulakshmi Suresh +3 more
doaj +1 more source
Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans [PDF]
, 2018 Background: Advances in automated image-based microscopy platforms coupled with high-throughput liquid workflows have facilitated the design of large-scale screens utilising multicellular model organisms such as Caenorhabditis elegans to identify genetic
Artal Sanz, Marta +10 more
core +1 more source
Plecstatin inhibits hepatocellular carcinoma tumorigenesis and invasion through cytolinker plectin
Molecular Oncology, EarlyView.The ruthenium‐based metallodrug plecstatin exerts its anticancer effect in hepatocellular carcinoma (HCC) primarily through selective targeting of plectin. By disrupting plectin‐mediated cytoskeletal organization, plecstatin inhibits anchorage‐dependent growth, cell polarization, and tumor cell dissemination.
Zuzana Outla +10 more
wiley +1 more source
Memorias do Instituto Oswaldo CruzBACKGROUND Bacillus Calmette-Guérin (BCG) is considered a promising live bacterial delivery system. However, several proposals for rBCG vaccines have not progressed, mainly due to the limitations of the available expression systems.
Larissa V Nascimento +3 more
doaj +1 more source
PLoS ONE, 2014 Background and aimGreen fluorescent protein (GFP) is a widely used molecular tag to trace transplanted cells in rodent liver injury models. The differing results from various previously reported studies using GFP could be attributed to the immunogenicity
Hiromichi Maeda +8 more
doaj +1 more source
Expression of Green Fluorescence Protein (GFP) in Zebrafish Muscle through Injection: A Gene Therapy Model [PDF]
, 2008 Expression of the target gene is important for gene therapy. Presently, localized transgenesis is used for gene therapy which can be achieved by a target gene expression. Here, we have reported the plasmid mediated gene therapy to zebrafish model.
C. H. Hsu +4 more
core +2 more sources
Red GFP and endogenous porphyrins [PDF]
Current Biology, 1999 This work has been supported by the Italian Association for Cancer Research (AIRC) and the Italian National Research Council (CNR – Convenzione Consorzio Mario Negri Sud). We thank T. Parasassi and F. Lorenzini for help during this work, C.A. Roessner for supplying the cysG gene, M. Anderson and L.A. Herzenberg for discussion.
Sacchetti, A. +5 more
openaire +2 more sources