Results 81 to 90 of about 520,090 (317)

Red GFP and endogenous porphyrins [PDF]

Current Biology, 1999
This work has been supported by the Italian Association for Cancer Research (AIRC) and the Italian National Research Council (CNR – Convenzione Consorzio Mario Negri Sud). We thank T. Parasassi and F. Lorenzini for help during this work, C.A. Roessner for supplying the cysG gene, M. Anderson and L.A. Herzenberg for discussion.
Sacchetti, A., Cappetti, V., Crescenzi, C., Celli, N., Rotilio, D., Alberti, S.   +5 more
openaire   +2 more sources

EDNRB‐dependent endothelin signaling reduces proliferation and promotes proneural‐to‐mesenchymal transition in gliomas

Molecular Oncology, EarlyView.
Glioma cells mainly express the endothelin receptor EDNRB, while EDNRA is restricted to a perivascular tumor subpopulation. Endothelin signaling reduces glioma cell proliferation while promoting migration and a proneural‐to‐mesenchymal transition associated with poor prognosis. This pathway activates Ca2+, K+, ERK, and STAT3 signalings and is regulated
Donovan Pineau, Leonor Garcia, Hugo Arnold, Antonija Hanžek, Maialen Arrieta, Laurent R Gauthier, Christine Granotier‐Beckers, François D Boussin, Amaury Herbet, Marie Hautière, Valentin Asei‐Ceschino, Clémentin Jacques, Laura Brard, Thomas Harnois, Valérie Coronas, Bruno Constantin, Aurélien Chatelier, Jean Chemin, Serge Urbach, Martial Seveno, Szimonetta Hideg, Chantal Ripoll, Kasandra Aguilar‐Cázarez, Min Zheng, Guo‐Hao Huang, Sheng‐Qing Lv, Lei Zhang, Philippe Rondard, Laurent Prezeau, Jean‐Philippe Pin, Hugues Duffau, Luc Bauchet, Valérie Rigau, Franck Denat, Charles Truillet, Didier Boquet, Jean‐Philippe Hugnot   +36 more
wiley   +1 more source

GFP-positive cells during pituitary development.

, 2016
Using sagittal sections of embryonic pituitaries on E18.5 (A–Bʹʹʹ) and E19.5 (C–Eʹʹʹ), immunostaining was performed for GFP, PRRX1, and SOX2, which were visualized with FITC (green), Cy3 (red), and Cy5 (white), respectively. GFP/PRRX1/SOX2-triple (yellow
Hideji Yako (3190110), Takashi Yashiro (3190128), Naoko Kanno (3190125), Saishu Yoshida (3190119), Takako Kato (2388967), Kotaro Horiguchi (3190134), Hiroki Ueharu (3190131), Ken Fujiwara (3190122), Takehiro Tsukada (3190113), Hiroto Nishihara (3190116), Yukio Kato (430483)   +10 more
core   +1 more source

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

Emergence of GFP-expressing PCs in SCA1 mouse cerebella after transplantation of TRE-GFP-hfMSCs expressing GFP through the Tet-off system.

, 2016
(a) The TRE-GFP gene was introduced into hfMSCs through a lentivirus (1). The transduced TRE-GFP-hfMSCs were injected into the cerebella of 6–8-month-old SCA1 mice (2).
Nobutaka Takahashi (1930330), Fathul Huda (3325365), Yasunori Matsuzaki (115418), Ayumu Konno (3091014), Yiping Fan (47091), Hirokazu Hirai (115426), Mamiko Suzuki (225727), Jerry K. Y. Chan (358275)   +7 more
core   +1 more source

GFP-CD9 expression in stable cell line.

, 2022
Cells from the GFP-CD9+ cell line were stained with Hoechst 33342 1:2,000 (in blue). Images were taken with a 40X objective. Expression of the fusion protein can be seen located to the cell membrane. (TIF)
Cristina Bellotti (12225254), Robert Steinfeld (7524614), Andreas Stäuble (12225257)   +2 more
core   +1 more source

Characterisation of alternative expression vectors for recombinant Bacillus Calmette-Guérin as live bacterial delivery systems

Memorias do Instituto Oswaldo Cruz
BACKGROUND Bacillus Calmette-Guérin (BCG) is considered a promising live bacterial delivery system. However, several proposals for rBCG vaccines have not progressed, mainly due to the limitations of the available expression systems.
Larissa V Nascimento, Carina C Santos, Luciana CC Leite, Ivan P Nascimento   +3 more
doaj   +1 more source

Disappearance of GFP-positive hepatocytes transplanted into the liver of syngeneic wild-type rats pretreated with retrorsine.

PLoS ONE, 2014
Background and aimGreen fluorescent protein (GFP) is a widely used molecular tag to trace transplanted cells in rodent liver injury models. The differing results from various previously reported studies using GFP could be attributed to the immunogenicity
Hiromichi Maeda, Masatoshi Shigoka, Yongchun Wang, Yingxin Fu, Russell N Wesson, Qing Lin, Robert A Montgomery, Hideaki Enzan, Zhaoli Sun   +8 more
doaj   +1 more source

Establishment of a humanized patient‐derived xenograft mouse model of high‐grade serous ovarian cancer for preclinical evaluation of combination immunotherapy

Molecular Oncology, EarlyView.
We have established a humanized orthotopic patient‐derived xenograft (Hu‐oPDX) mouse model of high‐grade serous ovarian cancer (HGSOC) that recapitulates human tumor–immune interactions. Using combined anti‐PD‐L1/anti‐CD73 immunotherapy, we demonstrate the model's improved biological relevance and enhanced translational value for preclinical ...
Luka Tandaric, Line Bjørge, Martine Rott Lode, Cecilie Fredvik Torkildsen, Pia Aehnlich, Rammah Elnour, Daniela Elena Costea, Lars Andreas Akslen, Liv Cecilie Vestrheim Thomsen, Emmet McCormack, Katrin Kleinmanns   +10 more
wiley   +1 more source

CD47 promotes mitogen‐activated protein kinase and epithelial‐to‐mesenchymal transition molecular programs to drive prometastatic phenotypes in non‐small cell lung cancer

Molecular Oncology, EarlyView.
Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau, Lilian G. Zhai, Ryunosuke Hoshi, Zackary Rousseau, Abraam Zakhary, Yin Fang Wu, Rola M. Saleeb, Heyu Ni, Kelsie L. Thu   +8 more
wiley   +1 more source