Results 101 to 110 of about 520,090 (317)
Molecular Oncology, EarlyView.We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober +16 more
wiley +1 more source
Design and validation of rAAV2-GFP-P2A-CCN5.
, 2022 (A). A schematic representation of rAAV2-GFP-P2A-CCN5. (B). Immunostaining of GFP in transverse retinal sections. GFP expression was detectable from ganglion cell to RPE layer. Scale bar, 100 μm. (C, D).
Ji Hong Bang (12867303) +8 more
core +1 more source
Finding novel vulnerabilities of hypomorphic BRCA1 alleles
Molecular Oncology, EarlyView.Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder +10 more
wiley +1 more source
, 2023 Long digitorum flexor tendons from 11 to 12-week-old Scx-GFP Tg rats were harvested, paraformaldehyde-fixed, freeze-embedded, and sectioned (5μm). Sections were then stained with anti-GFP antibody to detect Scx-expressing cells, as reflected by GFP ...
Katsumasa Ideo (6654218) +29 more
core +1 more source
Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization
Molecular Oncology, EarlyView.In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu +13 more
wiley +1 more source
, 2016 Primer name and sequence used for cloning sEH into GFP expression vector as well as primers used to mutate the C-terminal of sEH to either end in a canonical peroxisome targeting signal (Serine-Lysine-Leucine, SKL) or without a peroxisome targeting ...
Jonathan W. Nelson (2802394) +3 more
core +1 more source
Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer
Molecular Oncology, EarlyView.Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola +11 more
wiley +1 more source
Anti-Toxoplasma activity of P. betle extract on RH-GFP and PLK-GFP
, 2016 Anti-Toxoplasma activity of the P. betle extract on intracellular parasites RH-GFP (A) and PLK-GFP (B). The RH-GFP and PLK-GFP-infected HFF cells were treated with the P. betle extract for 72 h at different concentrations from 0 to 50 μg/mL.
Naomi Shimoda (2805220) +3 more
core +1 more source
, 2023 A: Heat map of collagens showing L2FC in DND1-GFP-lo vs DND1-GFP-hi cells from hiloRNA-seq (E16.5, E18.5). B: Enrichment of transcript as DND1 targets as a function of L2FC in RIP-seq (E14.5, E16.5, E18.5).
Jack D. Keene (14701357) +7 more
core +1 more source
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
FEBS Open Bio, EarlyView.Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source