Results 111 to 120 of about 520,090 (317)

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

GFP-Wdr68 overexpression increases the level of DYRK1A.

, 2018
A) C2C12 sublines mock (-) or transiently transfected with pEGFP-Wdr68 (GW) in GM. Lanes 1–2 are NT1 control cells. Lanes 3–4 are Δwdr68-9 (Δ9) cells. GFP-Wdr68 panel: Lanes 1 and 3, GFP-Wdr68 fusion protein was absent from mock-transfected cells.
Estibaliz Alvarado (275856), Yang Yu (4292), Jingyi Xu (6522), Robert M. Nissen (275861), David Salven (6029990), Mina Yousefelahiyeh (3360959)   +5 more
core   +1 more source

Characterization of a split-GFP-based approach to label membrane-expressed Ppk1.

, 2023
Representative images of ddaC neurons in live 3rd instar larvae. (A) By itself, secGFP(1–10) is not fluorescent (DcG-Ga/4 UAS-secGFP(1–10)) (top row). GFP(11) is not fluorescent in the absence of GFP(1–10) (ppk1:: GFP(11x3)Ec::mCher,yG-1erm)(middle row).
Ethan Schauer (17364787), Josephine W. Mitchell (17364781), Jill Wildonger (131997), Chun Han (1947577), Ipek Midillioglu (17364784), Bei Wang (116772)   +5 more
core   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

GFP-PfCSP interacts with heparin.

, 2016
A, Domain structures of full-length and recombinant PfCSP. The termini are indicated. SP, signal peptide; NTD, N-terminal domain; CTD, C-terminal domain; RI, region I; RII+, region II plus; RIII, region III; GPI, GPI anchor sequence.
Purnima Bhanot (272446), Qian Wang (32718), Jinghua Zhao (266787), Junjie Hu (383516)   +3 more
core   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

(A) Expression of GFP in liver sections at several times after infection of CYP2D6 mice with Ad-GFP

, 2011
Bars, 50 μm. (B) Serum levels of ALT and AST in humanized CYP2D6 and wild-type FVB/N mice infected with Ad-2D6 or Ad-GFP at several times after infection. Note that elevations in serum ALT and AST were transient and not persistent. Statistical evaluation
Edith Hintermann (92939), Monika Bayer (92940), Evelyn Rodrigo (92942), Urs Christen (92949), Michael P. Manns (92947), Antje Rhode (92941), Gudrun Hintereder (92943), Eric F. Johnson (92944), Frank J. Gonzalez (92945), Josef Pfeilschifter (92946), Matthias von G. Herrath (92948), Martin Holdener (92938)   +11 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source