Results 1 to 10 of about 391,068 (308)

TLR5-dependent immunogenicity of a recombinant fusion protein containing an immunodominant epitope of malarial circumsporozoite protein and the FliC flagellin of Salmonella Typhimurium [PDF]

Memorias do Instituto Oswaldo Cruz, 2011
Recently, we described the improved immunogenicity of new malaria vaccine candidates based on the expression of fusion proteins containing immunodominant epitopes of merozoites and Salmonella enterica serovar Typhimurium flagellin (FliC) protein as an ...
Ariane Guglielmi Ariza Camacho, Laís Helena Teixeira, Daniel Youssef Bargieri, Silvia Beatriz Boscardin, Irene da Silva Soares, Ruth Sonntag Nussenzweig, Victor Nussenzweig, Mauricio Martins Rodrigues   +7 more
doaj   +6 more sources

Application of a novel fusion tag system for enhanced soluble expression of recombinant proteins in Escherichia coli [PDF]

BMC Biotechnology
Background The Escherichia coli expression system is widely used for recombinant protein production, but its utility is often limited by the formation of insoluble inclusion bodies.
Li-Zhen Luo, Jian-Tao Cai, Zi-Ying Tan, Yu-Qing Chen, Zhe Hu, Hai-Hong Wang, Wen-Bin Zhang, Jin-Cheng Ma   +7 more
doaj   +2 more sources

A standardized set of pNX vectors for enhanced soluble expression of recombinant proteins in E. coli using small fusion tags [PDF]

Microbial Cell Factories
Background The production of recombinant proteins in Escherichia coli (E. coli) is often hampered by the formation of inclusion bodies. While fusion tags can enhance solubility, existing systems are hampered by a lack of standardization, with tags ...
Li-Zhen Luo, Wen-Bin Zhang, Zhe Hu, Ling-Hua Zhang, Jin-Cheng Ma, Xue-Bin Jiang   +5 more
doaj   +2 more sources

Production of recombinant SARS-COV-2 proteins and diphtheria toxoid CRM197-based fusion [PDF]

The Ukrainian Biochemical Journal, 2021
The quickly emerged global COVID-19 pandemic raised a desperate need in the development of protecting vaccines targeting this disease. Therefore, a generation of effective producers of recombinant SARS-CoV-2 proteins became an urgent task.
O. I. Krynina, S. I. Romaniuk, O. B. Gorbatiuk, O. H. Korchynskyi, А. V. Rebriiev, Ya. S. Kulyk, Ye. O. Kozadaieva, A. A. Siromolot, M. M. Guzyk, D. V. Kolybo, S. V. Komisarenko   +10 more
doaj   +1 more source

Fusion with heat-resistant obscure (Hero) proteins have the potential to improve the molecular property of recombinant proteins

PLoS ONE, 2022
Although recombinant proteins are widely used in biotechnology and pharmaceutical industries, improving their solubility and stability is often a challenging issue.
Eri Morimoto, Kotaro Tsuboyama, Yukihide Tomari   +2 more
doaj   +2 more sources

The B1 Domain of Streptococcal Protein G Serves as a Multi-Functional Tag for Recombinant Protein Production in Plants

Frontiers in Plant Science, 2022
Plants have long been considered a cost-effective platform for recombinant production. A recently recognized additional advantage includes the low risk of contamination of human pathogens, such as viruses and bacterial endotoxins. Indeed, a great advance
Shi-Jian Song, Hai-Ping Diao, Byeongho Moon, Areum Yun, Inhwan Hwang   +4 more
doaj   +1 more source

Pharmacokinetics of recombinant bifunctional fusion proteins [PDF]

Expert Opinion on Drug Metabolism & Toxicology, 2012
The development of biotechnology has enabled the creation of various recombinant fusion proteins as a new class of biotherapeutics. The uniqueness of fusion proteins lies in their ability to fuse two or more protein domains, providing vast opportunities to generate novel combinations of functions.
Xiaoying, Chen, Jennica L, Zaro, Wei-Chiang, Shen   +2 more
openaire   +2 more sources

Study of native SMAC protein production in the pUbiq expression system: Molecular cloning, biosynthesis and molecular modelling

Electronic Journal of Biotechnology, 2022
Background: In the process of recombinant protein biosynthesis affinity tags are efficient tools to achieve the expected purity and yield during the purification steps.
Pál Salamon, Csongor K. Orbán, Katalin Molnár-Nagy, Zita Kovács, Klára Váncsa, Emese Bálint, Ildikó Miklóssy, Beáta Albert, Gyöngyi Tar, Szabolcs Lányi   +9 more
doaj   +1 more source

Refinement of the Fusion Tag PagP for Effective Formation of Inclusion Bodies in Escherichia coli

Microbiology Spectrum, 2023
Methods for efficient insoluble protein production require further exploration. PagP, an Escherichia coli outer membrane protein with high β-sheet content, could function as an efficient fusion partner for inclusion body-targeted expression of ...
Xuefeng Li, Baorong Zhang, Quan Hu, Changchao Chen, Jiahua Huang, Lu Liu, Shengbin Wang   +6 more
doaj   +1 more source

Osteoinductive recombinant silk fusion proteins for bone regeneration [PDF]

Acta Biomaterialia, 2017
Protein polymers provide a unique opportunity for tunable designs of material systems due to the genetic basis of sequence control. To address the challenge of biomineralization interfaces with protein based materials, we genetically engineered spider silks to design organic-inorganic hybrid systems.
Dinjaski, N, Plowright, R, Zhou, S, Belton, DJ, Perry, CC, Kaplan, DL   +5 more
openaire   +3 more sources