PLoS ONE, 2022 Although recombinant proteins are widely used in biotechnology and pharmaceutical industries, improving their solubility and stability is often a challenging issue.
Eri Morimoto +2 more
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Fusion Partner and ER-Retention Signal Are Associated with Distinct Host Proteomic and Metabolic Responses During Interleukin-15 Production in Nicotiana benthamiana [PDF]
PlantsPlant molecular farming has emerged as a promising platform to produce recombinant biopharmaceuticals. The expression of foreign proteins changes the host proteome and metabolome, and these changes might have an effect on the accumulation of recombinant ...
Alina Savinova +3 more
doaj +2 more sources
Vesicle Nucleation Peptide Fusion Induced Extracellular Vesicles Are Distinct From Escherichia coli Outer Membrane Vesicles, and Provide an Enhanced Platform for Protein Production and Purification [PDF]
Journal of Extracellular VesiclesBacterial outer membrane vesicles (OMVs) are nano‐sized, spherical structures released by Gram‐negative bacteria that play diverse roles in bacterial physiology, including communication, nutrient acquisition and host interactions. These vesicles bud from
Bree R. Streather +9 more
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Fusion with immunoglobulin Fc fragment enhances the immunogenicity of the African swine fever virus p30/p54 fusion protein [PDF]
Applied Microbiology and BiotechnologyAfrican swine fever (ASF) is a significant threat to the global pig breeding industry, making the development of a vaccine extremely urgent. In this study, the key antigenic proteins p30 and p54 of African swine fever virus (ASFV) were fused with the Fc ...
Haimi Dang +12 more
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Catcher Domains as Multifunctional Fusion Modules for Soluble Expression, Covalent Coupling, and Spatial Organization of Recombinant Proteins [PDF]
Small ScienceFusion tags are essential tools for recombinant protein production, but their functions are often limited to improving solubility or facilitating purification.
Ruxia Fan +6 more
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Production of recombinant SARS-COV-2 proteins and diphtheria toxoid CRM197-based fusion [PDF]
The Ukrainian Biochemical Journal, 2021 The quickly emerged global COVID-19 pandemic raised a desperate need in the development of protecting vaccines targeting this disease. Therefore, a generation of effective producers of recombinant SARS-CoV-2 proteins became an urgent task.
O. I. Krynina +10 more
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Frontiers in Plant Science, 2022 Plants have long been considered a cost-effective platform for recombinant production. A recently recognized additional advantage includes the low risk of contamination of human pathogens, such as viruses and bacterial endotoxins. Indeed, a great advance
Shi-Jian Song +4 more
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Electronic Journal of Biotechnology, 2022 Background: In the process of recombinant protein biosynthesis affinity tags are efficient tools to achieve the expected purity and yield during the purification steps.
Pál Salamon +9 more
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Refinement of the Fusion Tag PagP for Effective Formation of Inclusion Bodies in Escherichia coli
Microbiology Spectrum, 2023 Methods for efficient insoluble protein production require further exploration. PagP, an Escherichia coli outer membrane protein with high β-sheet content, could function as an efficient fusion partner for inclusion body-targeted expression of ...
Xuefeng Li +6 more
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DEVELOPMENT OF THE VACCINE BASED ON THE RECOMBINANT ANTIGENS OF PSEUDOMONAS AERUGINOSA
Журнал микробиологии, эпидемиологии и иммунобиологии, 2019 Aim. The aim is obtaining, investigation and selection of recombinant antigens for inclusion theirs into the against Pseudomonas vaccine. Materials and methods. The genes encoding of the outer membrane proteins F, L and I and Exotoxin A were synthesized
N. A. Mihailova +3 more
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