Results 11 to 20 of about 2,843,513 (257)

Improvement of downstream processing of recombinant proteins by means of genetic engineering [PDF]

, 1993
Flaschel E, Friehs K. Improvement of downstream processing of recombinant proteins by means of genetic engineering. Biotechnology Advances. 1993;11(1):31-77.The rapid advancement of genetic engineering has allowed to produce an impressive number of ...
Flaschel, Erwin, Friehs, Karl
core   +1 more source

Soluble Form Production of Recombinant Human Insulin-Like Growth Factor-1 by NusA Fusion Partner in E. coli

Trends in Peptide and Protein Sciences, 2019
Insulin-Like Growth Factor-1 (IGF-1) is a small peptide with 70 amino acids and 7.6 kDa molecular weight that acts as the major mediator of growth hormone. According to the previous studies, recombinant production of human IGF-1 (rhIGF-1) in E.
Sara Hemmati, Javad Ranjbari
doaj   +1 more source

Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein [PDF]

, 2010
Background: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes.
Wels, Winfried, Pranav Oberoi, Jabulowsky, Robert Alexander, Benhar, Itai, Benjamin Dälken, Oberoi, Pranav, Robert A Jabulowsky, Itai Benhar, Winfried S Wels, Dälken, Benjamin   +9 more
core   +1 more source

Adjuvant-free immunization with hemagglutinin-Fc fusion proteins as an approach to influenza vaccines [PDF]

, 2011
The hemagglutinins (HAs) of human H1 and H3 influenza viruses and avian H5 influenza virus were produced as recombinant fusion proteins with the human immunoglobulin Fc domain.
Temperton, Nigel J., Ian M. Jones, Christopher R. Bailey, Eleonora Molesti, Ren, Junyuan, Jones, Ian M., Temperton, Nigel J, Silvia Loureiro, Pongsathon Phapugrangkul, Junyuan Ren, Wendy S. Barclay, Camilo A. Colaco, Molesti, Eleonora, Barclay, Wendy S., Shelton, Holly, Colaco, Camilo A, Phapugrangkul, Pongsathon, Nigel J. Temperton, Loureiro, Silvia, Barclay, Wendy S, Bailey, Christopher R, Holly Shelton, Jones, Ian, Colaco, Camilo A., Bailey, Christopher R.   +24 more
core   +1 more source

Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast

BMC Biotechnology, 2009
Background Expression of recombinant proteins in green algal chloroplast holds substantial promise as a platform for the production of human therapeutic proteins.
Henry Ryan E, Muto Machiko, Mayfield Stephen P   +2 more
doaj   +1 more source

Production of Recombinant Echinococcus granulosus Antigen B Subunits, In Order to Using Of Them in Serodiagnostic Tests of Hydatidosis [PDF]

Iranian Journal of Public Health, 2007
Background: Hydatidosis is one of the most important helminthiasis, and is a public health problem in many regions of the world. Methods: With the aim of production of recombinant subunits of antigen B, two different sequences of Echinococcus granulosus
R Pazoki, B Kazemi, MR Nazari, J Masoud, MR Akbari   +4 more
doaj   +1 more source

Expression of recombinant multi-coloured fluorescent antibodies in gor ^-/trxB^- E. coli cytoplasm [PDF]

, 2011
Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V., Burchell, Joy Marilyn; id_orcid, Kang, Angray S., Anatoliy Markiv, Burchell, J, Durvasula, R.V., Beatson, R., Beatson Richard, Beatson, Richard; id_orcid, Kang Angray S, Markiv, A., Markiv Anatoliy, Kang, A.S., Richard Beatson, Burchell, J., Durvasula Ravi V, Ravi V Durvasula, Burchell, Joy; id_orcid, Durvasula, RV, Markiv, Anatoliy, Burchell Joy, Markiv, A, Angray S Kang, Beatson, Richard, Joy Burchell, Kang, AS, Beatson, R   +26 more
core   +1 more source

Co-expression with the Type 3 Secretion Chaperone CesT from Enterohemorrhagic E. coli Increases Accumulation of Recombinant Tir in Plant Chloroplasts

Frontiers in Plant Science, 2017
Type 3 secretion systems (T3SSs) are utilized by pathogenic Escherichia coli to infect their hosts and many proteins from these systems are affected by chaperones specific to T3SS-containing bacteria.
Jacqueline MacDonald, Sean Miletic, Sean Miletic, Typhanie Gaildry, Typhanie Gaildry, Adam Chin-Fatt, Adam Chin-Fatt, Rima Menassa   +7 more
doaj   +1 more source

Plant-Based Cell-Free Transcription and Translation of Recombinant Proteins

, 2022
113124Plant cell-free lysates contain all the cellular components of the protein biosynthesis machinery, providing an alternative to intact plant cells, tissues, and whole plants for the production of recombinant proteins. Cell-free lysates achieve rapid
Schillberg, Stefan, Finnern, Ricarda, Buntru, Matthias, Vogel, Simon   +3 more
core   +1 more source

HaloTag is an effective expression and solubilisation fusion partner for a range of fibroblast growth factors [PDF]

PeerJ, 2015
The production of recombinant proteins such as the fibroblast growth factors (FGFs) is the key to establishing their function in cell communication. The production of recombinant FGFs in E.
Changye Sun, Yong Li, Sarah E. Taylor, Xianqing Mao, Mark C. Wilkinson, David G. Fernig   +5 more
doaj   +2 more sources