Results 21 to 30 of about 2,843,513 (257)

Mycobacterium tuberculosis antigen 85B and ESAT-6 expressed as a recombinant fusion protein in Mycobacterium smegmatis elicits cell-mediated immune response in a murine vaccination model [PDF]

, 2013
This is the post-print version of the final paper published in Molecular Immunology. The published article is available from the link below. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting ...
Kishore, U, Leiva, S, Robertson, BD, Rosenkrands, I, Nagy, J, Tsolaki, AG   +5 more
core   +1 more source

Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]

, 1993
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Seißler, Jochen, Mauch, Ludwig, Walter, Michael, Northemann, Wolfgang, Berg, Heike   +4 more
core   +1 more source

Expression of Tumor Necrosis Factor-Related Apoptosis- Inducing Ligand Conjugated to the scFV Region of Anti-CD20 Antibody Using a Small Ubiquitin-related Modifier [PDF]

مجله علمی دانشگاه علوم پزشکی کردستان, 2023
Background and Aim: Conventional treatments have shown little success in cancer treatment. Using antibody fragments conjugated with specific proteins and targeting specific receptors is a new strategy for producing anticancer drugs.
mahsa rasekhian, MARYAM pourjalili, omid tavallaei   +2 more
doaj  

Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium limosum

Biotechnology for Biofuels, 2021
Background The interest in using methanol as a substrate to cultivate acetogens increased in recent years since it can be sustainably produced from syngas and has the additional benefit of reducing greenhouse gas emissions.
Maximilian Flaiz, Gideon Ludwig, Frank R. Bengelsdorf, Peter Dürre   +3 more
doaj   +1 more source

Expression of 6 × his-hGM-CSF fusion gene in larvae of silkworm (Bombyx mori) using recombinant baculovirus

浙江大学学报. 农业与生命科学版, 2005
Six × his-hGM-CSF fusion gene was inserted in silkworm baculovirus transfer vector pBacPAK8 to construct the recombinant transfer vector pBacPAKHis-GM-CSF.
ZHU Li-cheng, CHEN Jian, LIN Rong, JIN Yong-feng, ZHANG Yao-zhou   +4 more
doaj   +1 more source

Production of Recombinant Proteins by Agrobacterium-Mediated Transient Expression

, 2022
The agroinfiltration of plant tissue is a robust method that allows the rapid and transient expression of recombinant proteins. Using wild-type plants as biomass, agroinfiltration exploits the ability of plants to synthesize even complex multimeric ...
Schillberg, Stefan, Noelke, Greta, Nölke, Greta, Spiegel, Holger   +3 more
core   +1 more source

The induction of recombinant protein bodies in different subcellular compartments reveals a cryptic plastid-targeting signal in the 27 kD γ-zein sequence

Frontiers in Bioengineering and Biotechnology, 2014
Naturally-occurring storage proteins such as zeins are used as fusion partners for recombinant proteins because they induce the formation of ectopic storage organelles known as protein bodies (PBs) where the proteins are stabilized by intermolecular ...
Anna eHofbauer, Jenny ePeters, Elsa eArcalis, Thomas eRademacher, Johannes eLampel, Francois eEudes, Alessandro eVitale, Eva eStoger   +7 more
doaj   +1 more source

Inhibition of the Hantavirus Fusion Process by Predicted Domain III and Stem Peptides from Glycoprotein Gc. [PDF]

PLoS Neglected Tropical Diseases, 2016
Hantaviruses can cause hantavirus pulmonary syndrome or hemorrhagic fever with renal syndrome in humans. To enter cells, hantaviruses fuse their envelope membrane with host cell membranes.
Gonzalo P Barriga, Fernando Villalón-Letelier, Chantal L Márquez, Eduardo A Bignon, Rodrigo Acuña, Breyan H Ross, Octavio Monasterio, Gonzalo A Mardones, Simon E Vidal, Nicole D Tischler   +9 more
doaj   +1 more source

Increased Accumulation of Recombinant Proteins in Soybean Seeds via the Combination Strategy of Polypeptide Fusion and Suppression of Endogenous Storage Proteins

Agronomy, 2023
Soybean seeds show great potential as a safe and cost-effective host for the large-scale production of biopharmaceuticals and industrially important macromolecules.
Jing Yang, Yuanyu Zhang, Guojie Xing, Jia Wei, Lu Niu, Qianqian Zhao, Qinan Cai, Xiaofang Zhong, Xiangdong Yang   +8 more
doaj   +1 more source

TLR5-dependent immunogenicity of a recombinant fusion protein containing an immunodominant epitope of malarial circumsporozoite protein and the FliC flagellin of Salmonella Typhimurium

Memorias do Instituto Oswaldo Cruz, 2011
Recently, we described the improved immunogenicity of new malaria vaccine candidates based on the expression of fusion proteins containing immunodominant epitopes of merozoites and Salmonella enterica serovar Typhimurium flagellin (FliC) protein as an ...
Ariane Guglielmi Ariza Camacho, Laís Helena Teixeira, Daniel Youssef Bargieri, Silvia Beatriz Boscardin, Irene da Silva Soares, Ruth Sonntag Nussenzweig, Victor Nussenzweig, Mauricio Martins Rodrigues   +7 more
doaj   +1 more source