Results 21 to 30 of about 255,577 (262)

Increased Accumulation of Recombinant Proteins in Soybean Seeds via the Combination Strategy of Polypeptide Fusion and Suppression of Endogenous Storage Proteins

Agronomy, 2023
Soybean seeds show great potential as a safe and cost-effective host for the large-scale production of biopharmaceuticals and industrially important macromolecules.
Jing Yang, Yuanyu Zhang, Guojie Xing, Jia Wei, Lu Niu, Qianqian Zhao, Qinan Cai, Xiaofang Zhong, Xiangdong Yang   +8 more
doaj   +1 more source

TLR5-dependent immunogenicity of a recombinant fusion protein containing an immunodominant epitope of malarial circumsporozoite protein and the FliC flagellin of Salmonella Typhimurium

Memorias do Instituto Oswaldo Cruz, 2011
Recently, we described the improved immunogenicity of new malaria vaccine candidates based on the expression of fusion proteins containing immunodominant epitopes of merozoites and Salmonella enterica serovar Typhimurium flagellin (FliC) protein as an ...
Ariane Guglielmi Ariza Camacho, Laís Helena Teixeira, Daniel Youssef Bargieri, Silvia Beatriz Boscardin, Irene da Silva Soares, Ruth Sonntag Nussenzweig, Victor Nussenzweig, Mauricio Martins Rodrigues   +7 more
doaj   +1 more source

Coexpressing the Signal Peptide of Vip3A and the Trigger Factor of Bacillus thuringiensis Enhances the Production Yield and Solubility of eGFP in Escherichia coli

Frontiers in Microbiology, 2022
Many fusion tags have been developed to improve the expression of recombinant proteins. Besides the translocation of cargo proteins, the signal peptides (SPs) of some secretory proteins, such as the ssTorA and Iasp, have been used as an inclusion body ...
Jianhua Gao, Chunping Ouyang, Juanli Zhao, Yan Han, Qinghua Guo, Xuan Liu, Tianjiao Zhang, Ming Duan, Xingchun Wang, Chao Xu   +9 more
doaj   +1 more source

Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System

Viruses, 2018
The baculovirus expression system (BES) is considered to be a very powerful tool for the expression of numerous difficult to express vertebrate proteins.
Won Seok Gwak, Jae Bang Choi, Beom Ku Han, Sung Min Bae, Soo Dong Woo   +4 more
doaj   +1 more source

Generation of a vector suite for protein solubility screening

Frontiers in Microbiology, 2014
Recombinant protein expression has become an invaluable tool for academic and biotechnological projects. With the use of high-throughput screening technologies for soluble protein production, uncountable target proteins were produced in a soluble and ...
Agustin eCorrea, Claudia eOrtega, Gonzalo eObal, Pedro eAlzari, Renaud eVincentelli, Pablo Javier Oppezzo   +5 more
doaj   +1 more source

Recombinant protein production data after expression in the bacterium Escherichia coli

Data in Brief, 2016
Fusion proteins have become essential for the expression and purification of recombinant proteins in Escherichia coli. The metal-binding protein CusF has shown several features that make it an attractive fusion protein and affinity tag: ''Expression and ...
J. Enrique Cantu-Bustos, Kevin D. Cano del Villar, Teresa Vargas-Cortez, Jose Ruben Morones-Ramirez, Isaias Balderas-Renteria, Xristo Zarate   +5 more
doaj   +1 more source

Plasmid Display for Stabilization of Enzymes Inside the Cell to Improve Whole-Cell Biotransformation Efficiency

Frontiers in Bioengineering and Biotechnology, 2020
Recombinant whole-cell biocatalysts are widely used for biotransformation of valuable products. However, some key enzymes involved in biotransformation processes are unstable and cannot be easily expressed in the functional form.
Yunjeong Park, Jonghyeok Shin, Jinkyeong Yang, Hooyeon Kim, Younghun Jung, Hyunseok Oh, Yongjoon Kim, Jaehyeon Hwang, Myeongseo Park, Choongjin Ban, Choongjin Ban, Ki Jun Jeong, Sun-Ki Kim, Dae-Hyuk Kweon, Dae-Hyuk Kweon, Dae-Hyuk Kweon   +15 more
doaj   +1 more source

Development of alkaline phosphatase-scFv and its use for one-step enzyme-linked immunosorbent assay for His-tagged protein detection

Open Life Sciences, 2022
A histidine (His)-tag is composed of six His residues and typically exerts little influence on the structure and solubility of expressed recombinant fusion proteins.
He Shuzhen, Xu Ruixian, Yi Huashan, Chen Zhixin, Chen Congjie, Li Qiang, Han Qinqin, Xia Xueshan, Song Yuzhu, Xu Junwei, Zhang Jinyang   +10 more
doaj   +1 more source

Effect of active immunization with recombinant-derived goose INH-α, AMH, and PRL fusion protein on broodiness onset and egg production in geese (Anser cygnoides)

Poultry Science, 2021
: This study was conducted to investigate the potential effects of active immunization against recombinant-derived goose inhibin-α (INH-α), anti-Müllerian hormone (AMH), and prolactin (PRL) fusion protein on broodiness onset and egg production in geese ...
Y. Zhang, Z.Y. Chen, C. An, K.Q. Weng, Z.F. Cao, Q. Xu, G.H. Chen   +6 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source