Results 31 to 40 of about 2,843,513 (257)

IgG Fusion Proteins for Brain Delivery of Biologics via Blood–Brain Barrier Receptor-Mediated Transport

Pharmaceutics, 2022
The treatment of neurological disorders with large-molecule biotherapeutics requires that the therapeutic drug be transported across the blood–brain barrier (BBB). However, recombinant biotherapeutics, such as neurotrophins, enzymes, decoy receptors, and
Ruben J. Boado
doaj   +1 more source

Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System

Viruses, 2018
The baculovirus expression system (BES) is considered to be a very powerful tool for the expression of numerous difficult to express vertebrate proteins.
Won Seok Gwak, Jae Bang Choi, Beom Ku Han, Sung Min Bae, Soo Dong Woo   +4 more
doaj   +1 more source

Coexpressing the Signal Peptide of Vip3A and the Trigger Factor of Bacillus thuringiensis Enhances the Production Yield and Solubility of eGFP in Escherichia coli

Frontiers in Microbiology, 2022
Many fusion tags have been developed to improve the expression of recombinant proteins. Besides the translocation of cargo proteins, the signal peptides (SPs) of some secretory proteins, such as the ssTorA and Iasp, have been used as an inclusion body ...
Jianhua Gao, Chunping Ouyang, Juanli Zhao, Yan Han, Qinghua Guo, Xuan Liu, Tianjiao Zhang, Ming Duan, Xingchun Wang, Chao Xu   +9 more
doaj   +1 more source

Recombinant Cytokines from Plants [PDF]

, 2011
Plant-based platforms have been successfully applied for the last two decades for the efficient production of pharmaceutical proteins. The number of commercialized products biomanufactured in plants is, however, rather discouraging. Cytokines are small
Sirko, Agnieszka, Patrycja Redkiewicz, Vanek, Tomas, Agnieszka Sirko, Góra-Sochacka, Anna, Redkiewicz, Patrycja, Anna Góra-Sochacka, Tomas Vaněk   +7 more
core   +1 more source

Generation of a vector suite for protein solubility screening

Frontiers in Microbiology, 2014
Recombinant protein expression has become an invaluable tool for academic and biotechnological projects. With the use of high-throughput screening technologies for soluble protein production, uncountable target proteins were produced in a soluble and ...
Agustin eCorrea, Claudia eOrtega, Gonzalo eObal, Pedro eAlzari, Renaud eVincentelli, Pablo Javier Oppezzo   +5 more
doaj   +1 more source

The Viral Replication Complex Is Associated with the Virulence of Newcastle Disease Virus [PDF]

, 2010
Virulent strains of Newcastle disease virus ([NDV] also known as avian paramyxovirus type 1) can be discriminated from low-virulence strains by the presence of multiple basic amino acid residues at the proteolytic cleavage site of the fusion (F) protein.
Peeters, B.P.H., G. Koch, B. P. H. Peeters, Rottier, P.J.M., Dortmans, J.C.F.M., J. C. F. M. Dortmans, P. J. M. Rottier, Koch, G.   +7 more
core   +1 more source

Controlled delivery of membrane proteins to artificial lipid bilayers by nystatin-ergosterol modulated vesicle fusion

, 2006
The study of ion channels and other membrane proteins and their potential use as biosensors and drug screening targets require their reconstitution in an artificial membrane.
Zagnoni, M, Fisher, K H, Berry, R M, de Planque, M R R, Watts, A, Mendes, G P, Morgan, H, Sandison, M E   +7 more
core   +2 more sources

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Pseudotyping Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV): F Proteins from Group II NPVs are functionally analogous to AcMNPV GP64 [PDF]

, 2002
GP64, the major envelope glycoprotein of budded virions of the baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), is involved in viral attachment, mediates membrane fusion during virus entry, and is required for efficient ...
Marcel Westenberg, Lung, O., Gary W. Blissard, Westenberg, M., Just M. Vlak, Douwe Zuidema, Vlak, J.M., Zuidema, D., Blissard, G.W., Oliver Lung   +9 more
core   +1 more source

Recombinant protein production data after expression in the bacterium Escherichia coli

Data in Brief, 2016
Fusion proteins have become essential for the expression and purification of recombinant proteins in Escherichia coli. The metal-binding protein CusF has shown several features that make it an attractive fusion protein and affinity tag: ''Expression and ...
J. Enrique Cantu-Bustos, Kevin D. Cano del Villar, Teresa Vargas-Cortez, Jose Ruben Morones-Ramirez, Isaias Balderas-Renteria, Xristo Zarate   +5 more
doaj   +1 more source