Results 31 to 40 of about 391,068 (308)

Increased Accumulation of Recombinant Proteins in Soybean Seeds via the Combination Strategy of Polypeptide Fusion and Suppression of Endogenous Storage Proteins

Agronomy, 2023
Soybean seeds show great potential as a safe and cost-effective host for the large-scale production of biopharmaceuticals and industrially important macromolecules.
Jing Yang, Yuanyu Zhang, Guojie Xing, Jia Wei, Lu Niu, Qianqian Zhao, Qinan Cai, Xiaofang Zhong, Xiangdong Yang   +8 more
doaj   +1 more source

The induction of recombinant protein bodies in different subcellular compartments reveals a cryptic plastid-targeting signal in the 27 kD γ-zein sequence

Frontiers in Bioengineering and Biotechnology, 2014
Naturally-occurring storage proteins such as zeins are used as fusion partners for recombinant proteins because they induce the formation of ectopic storage organelles known as protein bodies (PBs) where the proteins are stabilized by intermolecular ...
Anna eHofbauer, Jenny ePeters, Elsa eArcalis, Thomas eRademacher, Johannes eLampel, Francois eEudes, Alessandro eVitale, Eva eStoger   +7 more
doaj   +1 more source

Coexpressing the Signal Peptide of Vip3A and the Trigger Factor of Bacillus thuringiensis Enhances the Production Yield and Solubility of eGFP in Escherichia coli

Frontiers in Microbiology, 2022
Many fusion tags have been developed to improve the expression of recombinant proteins. Besides the translocation of cargo proteins, the signal peptides (SPs) of some secretory proteins, such as the ssTorA and Iasp, have been used as an inclusion body ...
Jianhua Gao, Chunping Ouyang, Juanli Zhao, Yan Han, Qinghua Guo, Xuan Liu, Tianjiao Zhang, Ming Duan, Xingchun Wang, Chao Xu   +9 more
doaj   +1 more source

Recombinant Envelope-Proteins with Mutations in the Conserved Fusion Loop Allow Specific Serological Diagnosis of Dengue-Infections [PDF]

, 2015
Dengue virus (DENV) is a mosquito-borne flavivirus and a major international public health concern in many tropical and sub-tropical areas worldwide. DENV is divided into four major serotypes, and infection with one serotype leads to immunity against the
Barzon, Luisa, Niedrig, Matthias, Pacenti, Monia, Palu', Giorgio, Rockstroh, Alexandra, Ulbert, Sebastian   +5 more
core   +4 more sources

Antibody Fusion Proteins: Anti-CD22 Recombinant Immunotoxin Moxetumomab Pasudotox [PDF]

Clinical Cancer Research, 2011
Abstract Recombinant immunotoxins are fusion proteins that contain the cytotoxic portion of a protein toxin fused to the Fv portion of an antibody. The Fv binds to an antigen on a target cell and brings the toxin into the cell interior, where it arrests protein synthesis and initiates the apoptotic cascade.
Robert J, Kreitman, Ira, Pastan
openaire   +2 more sources

Characterization of structural and immunological properties of a fusion protein between flagellin from Salmonella and lumazine synthase from Brucella [PDF]

, 2017
Aiming to combine the flexibility of Brucella lumazine synthase (BLS) to adapt different protein domains in a decameric structure and the capacity of BLS and flagellin to enhance the immunogenicity of peptides that are linked to their structure, we ...
Berguer, Paula Mercedes, Biedma, Marina Elizabeth, Blancá, Bruno Martin, Cayet, D., Errea, Agustina Juliana, Goldbaum, Fernando Alberto, Hiriart, Yanina, Moreno, Griselda Noemí, Rinaldi, Jimena Julieta, Rossi, Andrés Hugo, Rumbo, Martín, Sirard, J.C.   +11 more
core   +3 more sources

Immunocontraceptive potential of a GnRH receptor-based fusion recombinant protein [PDF]

Journal of Genetic Engineering and Biotechnology, 2021
The management of stray dog population has been of utmost importance due to their overpopulation, increase in dog bites incidence, and rabies. Contraceptive vaccines, a non-surgical alternative to spaying and neutering are viewed as a valuable option for the management of dog population.
Nathaniel Philip Sandam, Dhamodhar Prakash, Prashanth Thimmareddy   +2 more
openaire   +3 more sources

Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System

Viruses, 2018
The baculovirus expression system (BES) is considered to be a very powerful tool for the expression of numerous difficult to express vertebrate proteins.
Won Seok Gwak, Jae Bang Choi, Beom Ku Han, Sung Min Bae, Soo Dong Woo   +4 more
doaj   +1 more source

The Use of Recombinant Human Bone Morphogenetic Protein 2 (rhBMP-2) to Promote Spinal Fusion in a Nonhuman Primate Anterior Interbody Fusion Model [PDF]

, 1999
Study Design. A study on the efficacy of recombinant human bone morphogenetic protein 2 (rhBMP-2) in a nonhuman primate anterior interbody fusion model. Objectives.
Fischgrund, Jeffrey S., Harkowitz, Harry N., Hecht, Brian P., Penman, Lori, Shirkhoda, Ali, Toth, Jeffrey M.   +5 more
core   +2 more sources

Generation of a vector suite for protein solubility screening

Frontiers in Microbiology, 2014
Recombinant protein expression has become an invaluable tool for academic and biotechnological projects. With the use of high-throughput screening technologies for soluble protein production, uncountable target proteins were produced in a soluble and ...
Agustin eCorrea, Claudia eOrtega, Gonzalo eObal, Pedro eAlzari, Renaud eVincentelli, Pablo Javier Oppezzo   +5 more
doaj   +1 more source