Recombinant whole-cell biocatalysts are widely used for biotransformation of valuable products. However, some key enzymes involved in biotransformation processes are unstable and cannot be easily expressed in the functional form.
Yunjeong Park +15 more
doaj +1 more source
Russell-like bodies in plant seeds share common features with prolamin bodies and occur upon recombinant protein production [PDF]
Although many recombinant proteins have been produced in seeds at high yields without adverse effects on the plant, endoplasmic reticulum (ER) stress and aberrant localization of endogenous or recombinant proteins have also been reported.
Arcalis, Elsa +13 more
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Regulation of the Neuron-specific Ras GTPase-activating Protein, synGAP, by Ca2+/Calmodulin-dependent Protein Kinase II [PDF]
synGAP is a neuron-specific Ras GTPase-activating protein found in high concentration in the postsynaptic density fraction from mammalian forebrain. Proteins in the postsynaptic density, including synGAP, are part of a signaling complex attached to the ...
Kennedy, Mary B. +2 more
core +1 more source
: This study was conducted to investigate the potential effects of active immunization against recombinant-derived goose inhibin-α (INH-α), anti-Müllerian hormone (AMH), and prolactin (PRL) fusion protein on broodiness onset and egg production in geese ...
Y. Zhang +6 more
doaj +1 more source
Baculovirus Per Os Infectivity Factors Form a Complex on the Surface of Occlusion-Derived Virus [PDF]
Five highly conserved per os infectivity factors, PIF1, PIF2, PIF3, PIF4, and P74, have been reported to be essential for oral infectivity of baculovirus occlusion-derived virus (ODV) in insect larvae.
Hu, Z. +4 more
core +2 more sources
Recombinant protein production data after expression in the bacterium Escherichia coli
Fusion proteins have become essential for the expression and purification of recombinant proteins in Escherichia coli. The metal-binding protein CusF has shown several features that make it an attractive fusion protein and affinity tag: ''Expression and ...
J. Enrique Cantu-Bustos +5 more
doaj +1 more source
A histidine (His)-tag is composed of six His residues and typically exerts little influence on the structure and solubility of expressed recombinant fusion proteins.
He Shuzhen +10 more
doaj +1 more source
Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella [PDF]
In this study, we evaluated the binding capacity of Type I MAR domains from novel E. tenella MCPs. Variants of the previously described HxT motif were analysed showing that HxT and VxT variants bind, whereas HxS and YxE variants did not. One of these MCP
Marugan-Hernandez, V +5 more
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The dysbindin-containing complex (BLOC-1) in brain: developmental regulation, interaction with SNARE proteins and role in neurite outgrowth. [PDF]
Previous studies have implicated DTNBP1 as a schizophrenia susceptibility gene and its encoded protein, dysbindin, as a potential regulator of synaptic vesicle physiology.
Chan, LN +9 more
core +2 more sources
Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli [PDF]
Recombinant protein production is a useful technology for therapeutic and diagnostic applications. The bacterium Escherichia coli is widely used for the bioproduction of proteins, but still presents some drawbacks.
Castro, António +2 more
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