Results 121 to 130 of about 520,090 (317)
Proceedings of the National Academy of Sciences, 2009 JOP. Journal of the Pancreas, Vol 15, No 5 (2014): September - p.
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FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source
FEBS Open Bio, EarlyView.KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura +19 more
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GFP-2x-TgPH1 retains membrane association in several mammalian cells inhibited for PIKfyve.
, 2018 Human PC3 cells and RPE cells were transfected with GFP or GFP-2x-TgPH1, labelled with fluorescent dextran to identify lysosomes and then treated with vehicle or apilimod to inhibit PIKfyve for 1 h, followed by spinning disc confocal imaging. A, B.
Roberto J. Botelho (5340947) +6 more
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Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Electrophoretic Mobility Shift Assays with GFP-Tagged Proteins (GFP-EMSA)
, 2019 The electrophoretic mobility shift assay (EMSA) is commonly used for the study of nucleic acid-binding proteins. The technique can be used to demonstrate that a protein is binding to RNA or DNA through visualization of a shift in electrophoretic mobility of the nucleic acid band.
Sorenson, Alanna E. +1 more
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FEBS Open Bio, EarlyView.TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source
GFP-PfRab1A fluorescence is found as discrete loci in living cells.
, 2016 A. GFP fluorescence is observed as discrete loci termed PfRab1A bodies in trophozoite and schizont stage parasites expressing GFP-PfRab1A. B. GFP-PfRab18 fluorescence is diffuse and closely associated with the nuclei. C.
Wesley Webster (3162885) +4 more
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Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
FEBS Open Bio, EarlyView.Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
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FEBS Open Bio, EarlyView.The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
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