Results 141 to 150 of about 520,090 (317)

Transformation of Phytophthora cinnamomi with the green Fluorescent Protein (GFP) for studies on survival

, 2005
For soilborne plant pathogens such as Phytophthora cinnamomi, differentiating between spores of P. cinnamomi and other similar microorganisms can be challenging, making it difficult to determine the form the pathogen survives in soils and host tissue ...
McCarren, K.L., O'Brien, P., Shearer, B., Hardy, G.E.St.J., McComb, J.A.   +4 more
core  

GFP-Vangl2 is enriched to the tip of retracting FBMN filopodia.

, 2016
(A) Live confocal image of a single GFP-Vangl2 expressing FBMN (green) in a Tg(isl1:mRFP) (magenta) 24 hpf embryo. Scale bar: 10 μm. (A’,A”) Magnified views of the boxed region in A of the individual channels, GFP-Vangl2 and Tg(isl1:mRFP) respectively ...
Cecilia B. Moens (159071), Crystal F. Davey (3176205), Andrew W. Mathewson (3176208)   +2 more
core   +1 more source

Differential Immunostimulatory Effects of EGFP and +36 GFP on Immune Cells

Journal of Medical Microbiology and Infectious Diseases
Introduction: Green fluorescent protein (GFP) and its variants are pivotal in tracking gene expression across various gene delivery systems. While GFP is typically employed for intracellular reporting, it can be modified to display on cell surfaces ...
Parisa Moradi Pordanjani1, Elnaz Agi2, Azam Bolhassani1⁎   +2 more
doaj   +1 more source

Thermally Modulated Metasurface Sensor for Dynamic and Time‐Resolved Isolation of Extracellular Vesicles

Advanced Materials, EarlyView.
A thermally adaptive plasmonic metasurface, functionalized with poly(N‐isopropylacrylamide) (PNIPAM) and anti‐CD63 antibodies, enables precise, on‐demand capture and release of extracellular vesicles (EVs). Delivering high recovery, improved purity, and intact vesicle structure, the platform offers a versatile, label‐free solution for real‐time EV ...
Beyza Nur Kucuk, Eylul Gulsen Yilmaz, Yusuf Aslan, Emre Ece, Özgecan Erdem, Murat Alp Gungen, Bengi Özgün Öztürk, Fatih Inci   +7 more
wiley   +1 more source

GFP-Rab11A intracellular movement in RSV infected A549 cells

, 2019
TIFF series of diSPIM datasets, fused and deconvolved, from A549 GFP-Rab11A cells infected with Respiratory Syncytial Virus. Each TIFF file is a full cell volume of 50 planes, with isotropic resolution of 330nm, each .zip file is an independent ...
Amar Bhagwat (7592216), Seema Lakdawala (7816838)   +1 more
core   +1 more source

Conductive Hydrogels for Exogenous Sensing and Cell Fate Control

Advanced Materials, EarlyView.
We engineer electrically conductive hydrogels by combining sulfated glycosaminoglycans with semiconducting polymers. These hydrogels bind bioactive proteins, including growth factors, whose release or retention can be modulated by low‐voltage stimulation. The hydrogels are also integrated as 3D channels in organic electrochemical transistors as part of
Teuku Fawzul Akbar, Carlos Alejandro Jimenez‐Rodriguez, Railia Biktimirova, Ilka Hermes, Thomas Kurth, My Duyen Pham, Mikhail Tsurkan, Jens Friedrichs, Francis L. C. Morgan, Hans Kleemann, Olga Guskova, Uwe Freudenberg, Peter Fratzl, Carsten Werner, Christoph Tondera, Ivan R. Minev   +15 more
wiley   +1 more source

A Reproducible and Scalable Process for High Yield Mesenchymal Stromal Cell Extracellular Vesicles Production

Advanced Materials Technologies, EarlyView.
Mesenchymal stem cell derived extracellular vesicles (MSC‐EVs) are a promising therapeutic tool for regenerative medicine. However, the field lacks a reproducible high‐yield production method to answer the quantities needed for clinical translation.
Christophe Wong, Elise Madec, Jeanne Volatron, Max Piffoux, Amanda K. A. Silva, Florence Gazeau, Jean‐Philippe Herbeuval, Thibaut Fourniols   +7 more
wiley   +1 more source

AI‐Powered Framework for Evaluating Drug Efficacy for Three‐Dimensional In Vitro Cancer Models in Robot‐Assisted Production

Advanced Robotics Research, EarlyView.
An AI‐powered, robot‐assisted framework automatically produces, images, and analyzes 3D tumor spheroids to evaluate drug efficacy. Integrated modules handle spheroid formation, live/dead staining, brightfield imaging, and automated image analysis, including spheroid segmentation, viability and metrics to assess the drug treatment efficacy. The workflow
Dalia Mahdy, Jesus Nieves, Lukas Königer, Matthias Peindl, Fabio Erhard, Marc Möllmann, Anke Klingner, Markus Münig, Bibin Babu, Thomas Schwarz, Marco Metzger, Gudrun Dandekar, Maximiliane Wußmann, Jan Hansmann   +13 more
wiley   +1 more source

Dual‐Functional Ingestible Passive Capsules for High‐Throughput Intestinal Sampling with Sealed Containment and Targeted Drug Delivery

Advanced Robotics Research, EarlyView.
To address challenges in high‐throughput intestinal sampling with sealed containment and target drug delivery, we developed a dual‐functional ingestible passive capsule with a dual‐triggered control system based on pH‐response and mechanical actuation.
Libing Huang, Houyu Ling, Xizong Chen, Yufei Duan, Yu Liu, Shuntian Cai, Yandan Ren, Wenlong Li, Lei Ren, Miao Wang   +9 more
wiley   +1 more source

Malectin Alleviates Endoplasmic Reticulum Stress in Gestational Diabetes Mellitus via Glycoprotein Quality Control Mechanisms

Advanced Science, EarlyView.
Malectin alleviates high glucose‐induced ER stress and damage in placental trophoblasts, a function dependent on its six critical carbohydrate‐binding residues. In a GDM mouse model, administration of TAT‐Malectin ameliorated hyperglycemia and placental ER stress and prevented fetal macrosomia.
Jiahui Zhu, Yumeng Zhang, Ye Wang, Xiaoyu Zhu, Ailin Yuan, Weijing Yin, Huangmin Yu, Xuemin Pang, Yufeng He, Yuchen Wang, Tong Wang, Yong Li, Yunlong Si   +12 more
wiley   +1 more source