Results 41 to 50 of about 520,090 (317)

Stochastic activation of the response regulator PhoB by noncognate histidine kinases

Journal of Integrative Bioinformatics, 2005
Two-component systems (TCS) are the most prevalent gene regulatory mechanism in bacteria. A typical TCS is comprised of a histidine kinase (HK) and a partner response regulator (RR).
Zhou Lu, Grégori Gérald, Blackman Jennifer Masella, Robinson J. Paul, Wanner Barry L.   +4 more
doaj   +1 more source

Aristaless-related homeobox plays a key role in hyperplasia of the pancreas islet α-like cells in mice deficient in proglucagon-derived peptides. [PDF]

PLoS ONE, 2013
Defects in glucagon action can cause hyperplasia of islet α-cells, however, the underlying mechanisms remain largely to be elucidated. Mice homozygous for a glucagon-GFP knock-in allele (Gcg(gfp/gfp) ) completely lack proglucagon-derived peptides and ...
Sai Xu, Yoshitaka Hayashi, Yoshiko Takagishi, Mariko Itoh, Yoshiharu Murata   +4 more
doaj   +1 more source

GFP-EhGEF expression and localization in amoebic cells during phagocytosis.

, 2021
(A) Western analysis of GFP -EhGEF. E. histolytica cell expressing GFP-EhGEF were induced with different concentration of G418(10, 20 and 30 μg/ml) for 48h. Each lane was loaded with 100 μg of cell lysate as shown in figure.
Ravi Bharadwaj (11740650), Tushar Kushwaha (9482480), Azhar Ahmad (11740653), Krishna K. Inampudi (11740656), Tomoyoshi Nozaki (131314), Somlata (11740659)   +5 more
core   +1 more source

GFP-Atg8 localization by fluorescence microscopy.

, 2020
KN99α+GFP-Atg8 and atg4+GFP-Atg8 strains were cultured in YPD and SD/-N/-AA at 30°C and 37°C for 4 h. Vacuole labeled with FM4-64 (Invitrogen) dye. (A) GFP-Atg8 localization during autophagy pathway. White arrows indicate the vacuole membrane. Scale bars
Renata Castiglioni Pascon (6612353), Thiago Nunes Roberto (8677083), Marcelo Afonso Vallim (5957576), Ricardo Ferreira Lima (8677086), Alexander Idnurm (58590)   +4 more
core   +1 more source

Presentation of antigen on extracellular vesicles using transmembrane domains from viral glycoproteins for enhanced immunogenicity

Journal of Extracellular Vesicles, 2022
A vaccine antigen, when launched as DNA or RNA, can be presented in various forms, including intracellular, secreted, membrane‐bound, or on extracellular vesicles (EVs).
Kai Hu, Paul F. McKay, Karnyart Samnuan, Adrian Najer, Anna K. Blakney, Junyi Che, Gwen O'Driscoll, Martina Cihova, Molly M. Stevens, Robin J. Shattock   +9 more
doaj   +1 more source

Examples of genes from PANTHER protein classes “Transcriptional Regulation”, “Defense/Immunity”, “Transporters”, and “Transmembrane Signal Receptors” that were significantly differentially expressed in DND1-GFP-lo compared to DND1-GFP-hi cells at E16.5 and/or E18.5.

, 2023
hiloRNA-seq expression P-value (DESeq2) between DND1-GFP-lo and DND1-GFP-hi cells at E16.5 and E18.5: not significant (ns), (TIF)
Jack D. Keene (14701357), Blanche Capel (16995), Victor A. Ruthig (14701345), Josiah Hardy (14701348), Talia Hatkevich (6639401), Matthew B. Friedersdorf (14701351), Chloé Mayère (14701354), Serge Nef (45552)   +7 more
core   +1 more source

Evidence of green fluorescent protein and growth hormone expression in red abalone (Haliotis rufescens) larvae

Open Agriculture, 2017
The red abalone Haliotis rufescens is a highly appreciated mollusk in the national and international markets. Due to its natural over-exploitation and low growth rate, several genetic improvements were made, however special efforts are needed to increase
Mancilla-Sánchez Edgar, Portillo-López Amelia   +1 more
doaj   +1 more source

Preliminary activity (see Fig 1A): Characterization of the pCMV3-GFP-Spike plasmid and the RNA produced by untreated A549 cells and A549 cells transfected with the pCMV3-GFP-Spike plasmid.

, 2022
A. 0.8% w/v agarose gel electrophoresis of the pCMV3-GFP-Spike plasmid. B. 0.8% w/v agarose gel electrophoresis of RNA isolated from untreated A549 cells or cells transfected with the pCMV3-GFP-Spike plasmid. C. Check on 1.8% w/v agarose gel of amplicons
Jessica Gasparello (2633761), Cristina Zuccato (717940), Matteo Zurlo (7372766), Giulia Breveglieri (717941), Lucia Carmela Cosenza (3779479), Roberto Gambari (328450), Alessia Finotti (717938), Chiara Papi (3013806)   +7 more
core   +1 more source

Subcellular localization of rice hexokinase (OsHXK) family members in the mesophyll protoplasts of tobacco

Biologia Plantarum, 2011
Hexokinase (HXK, EC 2.7.1.1) plays an important role in the metabolism and glucose signalling. To examine the characteristics of HXK gene family in rice, the subcellular localizations of ten hexokinases (OsHXK1 - OsHXK10) were determined using OsHXK::GFP
W. Cheng, H. Zhang, X. Zhou, H. Liu, Y. Liu, J. Li, S. Han, Y. Wang   +7 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source