Results 11 to 20 of about 520,090 (317)

Molecular and electrophysiological characterization of GFP-expressing CA1 interneurons in GAD65-GFP mice. [PDF]

open access: yesPLoS ONE, 2010
The use of transgenic mice in which subtypes of neurons are labeled with a fluorescent protein has greatly facilitated modern neuroscience research. GAD65-GFP mice, which have GABAergic interneurons labeled with GFP, are widely used in many research ...
Corette J Wierenga   +5 more
doaj   +5 more sources

Optimization of transient gene expression in tomato (Solanum lycopersicum L.) [PDF]

open access: yesمجله بیوتکنولوژی کشاورزی, 2023
ObjectiveThe aim of this study is to optimize tomato as a suitable plant system for transient gene transformation for the production of pharmaceutical and industrial compounds.Materials and methodsIn order to investigate transient gene expression, three ...
Masomeh Nasiri Mogadam   +3 more
doaj   +1 more source

Peculiarities of green fluorescent protein transgene detection in tobacco and maize plants by PCR [PDF]

open access: yesBiotechnologia Acta, 2023
The aim of the work was to investigate detection of different modifications of the green fluorescent protein gene (gfp) in the transgenic tobacco and maize plants by polymerase chain reaction (PCR). Methods.
Nitovska І. О.   +2 more
doaj   +1 more source

Downregulation of FeSOD-A expression in Leishmania infantum alters trivalent antimony and miltefosine susceptibility

open access: yesParasites & Vectors, 2021
Background Superoxide dismutase (SOD), a central component of the antioxidant defence system of most organisms, removes excess superoxide anions by converting them to oxygen and hydrogen peroxide. As iron (Fe) SOD is absent in the human host, this enzyme
Ana Maria Murta Santi   +3 more
doaj   +1 more source

The significance of C-terminal NLS sequences of VirD2 in its nuclear localization in Saccharomyces cerevisiae [PDF]

open access: yesJournal of Cell and Molecular Research, 2013
Agrobacterium tumefaciens is capable of gene transfer to both plant and non-plant organisms. Indeed, upon infection of eukaryotic cells, Agrobacterium tumefaciens transfers a piece of its tumor inducing (Ti)-plasmid, called T-DNA, to the host cell ...
Jalal Soltani   +3 more
doaj   +1 more source

Lentiviral vector-mediated transduction of adult neural stem/progenitor cells isolated from the temporal tissues of epileptic patients [PDF]

open access: yesIranian Journal of Basic Medical Sciences, 2020
Objective(s): Neural stem/progenitor cells (NS/PCs) hold a great potential for delivery of therapeutic agents into the injured regions of the brain.
Sara Abdollahi   +6 more
doaj   +1 more source

UV Light Induced Fluorescence Recovery of GFP After Photobleaching in Microscopy Imaging

open access: yesImage Analysis and Stereology, 2022
Fluorescence microscopy has become one of the most important tools for biologists to visualize and study organelles and molecules in a cell. Fluorescent markers are used to visualize specific molecules.
Griša G. Prinčič   +4 more
doaj   +1 more source

Although the germ cell transcriptome is mostly shared between DND1-GFP-lo and DND1-GFP-hi cells at E16.5 and E18.5, the subset of significant differentially expressed genes indicate functional differences between DND1-GFP-lo and DND1-GFP-hi cells.

open access: yes, 2023
A: Global shared (pink) versus DND1-GFP-lo (blue) or DND1-GFP-hi (yellow; red) specific expression at E16.5 and E18.5 from hiloRNA-seq analysis. B: Euler plots of E16.5 DND1-GFP-lo (blue), E16.5 DND1-GFP-hi (yellow), E18.5 DND1-GFP-lo (dark blue), E18.5 ...
Jack D. Keene (14701357)   +7 more
core   +1 more source

GFP-CENP-C, CID-GFP or CAL1-GFP in stage 14 oocytes.

open access: yes, 2023
The DNA is blue, microtubules in white, and the scale bars represent 5 μm. A) Localization of CID-GFP or CAL1-GFP (green) using the MVD1 or matα. In the one image (gcal1-GFP), CAL1-GFP is regulated by the endogenous cal1 promoter.
Aashka Parikh (17481074)   +6 more
core   +1 more source

Trypanin Disruption Affects the Motility and Infectivity of the Protozoan Trypanosoma cruzi

open access: yesFrontiers in Cellular and Infection Microbiology, 2022
The flagellum of Trypanosomatids is an organelle that contributes to multiple functions, including motility, cell division, and host–pathogen interaction.
Jose L. Saenz-Garcia   +10 more
doaj   +1 more source

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