Results 91 to 100 of about 1,023,137 (333)

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

Protein Transduction Method for Cerebrovascular Disorders [PDF]

, 2009
Many studies have shown that a motif of 11 consecutive arginines (11R) is one of the most effective protein transduction domains (PTD) for introducing proteins into the cell membrane.
Arimitsu, Seiji, Date, Isao, Ichikawa, Tomotsugu, Matsui, Hideki, Ogawa, Tomoyuki, Ono, Shigeki, Onoda, Keisuke, Sugiu, Kenji, Tokunaga, Koji, Tomizawa, Kazuhito   +9 more
core   +1 more source

Synthetic Control of Green Fluorescent Protein [PDF]

Journal of the American Chemical Society, 2009
Semisynthetic green fluorescent proteins (GFPs) can be prepared by producing truncated GFPs recombinantly and assembling them with synthetic beta-strands of GFP. The yield from expressing the truncated GFPs is low, and the chromophore is either partially formed or not formed. An alternative method is presented in which full-length proteins are produced
Kevin P, Kent, Luke M, Oltrogge, Steven G, Boxer   +2 more
openaire   +2 more sources

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Effects of reduced dissolved oxygen concentrations on physiology and fluorescence of hermatypic corals and benthic algae. [PDF]

, 2014
While shifts from coral to seaweed dominance have become increasingly common on coral reefs and factors triggering these shifts successively identified, the primary mechanisms involved in coral-algae interactions remain unclear. Amongst various potential
Deheyn, Dimitri D, Haas, Andreas F, Smith, Jennifer E, Thompson, Melissa   +3 more
core   +1 more source

Green fluorescent proteins as optically controllable elements in bioelectronics [PDF]

, 2001
A single-biomolecule optical toggle switch is demonstrated based on a mutated green fluorescent protein (GFP). We have exploited molecular biology techniques to tailor the GFP molecular structure and photophysical properties and to give it optically ...
Riccardo A. G. Cinelli, V. Pellegrini, A. Ferrari, P. Faraci, R. Nifosí, Mudit Tyagi, M. Giacca, F. Beltram   +7 more
semanticscholar   +1 more source

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

Sub-cellular trafficking of phytochemicals explored using auto-fluorescent compounds in maize cells

BMC Plant Biology, 2003
Background Little is known regarding the trafficking mechanisms of small molecules within plant cells. It remains to be established whether phytochemicals are transported by pathways similar to those used by proteins, or whether the expansion of ...
Grotewold Erich, Irani Niloufer G, Lin Yakang   +2 more
doaj   +1 more source

Visualizing mitochondrial FoF1-ATP synthase as the target of the immunomodulatory drug Bz-423

, 2018
Targeting the mitochondrial enzyme FoF1-ATP synthase and modulating its catalytic activities with small molecules is a promising new approach for treatment of autoimmune diseases. The immuno-modulatory compound Bz-423 is such a drug that binds to subunit
Börsch, Michael, Glick, Gary D., Starke, Ilka   +2 more
core   +1 more source