Results 31 to 40 of about 2,897,926 (346)

Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector

Nature Communications, 2018
Different fluorescence fluctuation methods require specialized equipment and cannot be performed simultaneously. Here the authors present an implementation that includes many advanced fluorescence fluctuation techniques in a single analysis, by ...
L. Scipioni, L. Lanzanó, A. Diaspro, E. Gratton   +3 more
doaj   +1 more source

AO DIVER: Development of a three-dimensional adaptive optics system to advance the depth limits of multiphoton imaging

APL Photonics, 2020
Multiphoton microscopy (MPM) can non-invasively measure the dynamic biochemical properties deep in scattering biological samples and has the potential to accelerate clinical research with advances in deep tissue imaging.
S. Leemans, A. Dvornikov, T. Gallagher, E. Gratton   +3 more
doaj   +1 more source

Detection and Quantification of DNA by Fluorophore-Induced Plasmonic Current: A Novel Sensing Approach

Sensors
We report on the detection and quantification of aqueous DNA by a fluorophore-induced plasmonic current (FIPC) sensing method. FIPC is a mechanism described by our group in the literature where a fluorophore in close proximity to a plasmonically active ...
Daniel R. Pierce, Zach Nichols, Clifton Cunningham, Sean Avryl Villaver, Abdullah Bajwah, Samuel Oluwarotimi, Herbert Halaa, Chris D. Geddes   +7 more
doaj   +1 more source

Optical Manipulation of Lanthanide-Doped Nanoparticles: How to Overcome Their Limitations

Frontiers in Chemistry, 2020
Since Ashkin's pioneering work, optical tweezers have become an essential tool to immobilize and manipulate microscale and nanoscale objects. The use of optical tweezers is key for a variety of applications, including single-molecule spectroscopy ...
Elisa Ortiz-Rivero, Lucía Labrador-Páez, Paloma Rodríguez-Sevilla, Patricia Haro-González, Patricia Haro-González   +4 more
doaj   +1 more source

Efficient nitrogen-vacancy centers' fluorescence excitation and collection from micrometer-sized diamond by a tapered optical fiber

, 2018
Efficiently excite nitrogen-vacancy (NV) centers in diamond and collect their fluorescence significantly benefit the fiber-optic-based NV sensors.
Arumugam, Sri Ranjini, Balasubramanian, Gopalakrishnan, Chang, Huan-Cheng, Du, Guanxiang, Duan, Dewen, Kavatamane, Vinaya Kumar, Tzeng, Yan-Kai   +6 more
core   +1 more source

Fluorescence Microscopy [PDF]

Cold Spring Harbor Protocols, 2014
Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution.
Sanderson, Michael J, Smith, Ian, Parker, Ian, Bootman, Martin D   +3 more
openaire   +4 more sources

Biological and technical complexities in analyzing extracellular vesicle immune interactions in B‐cell malignancies

FEBS Letters, EarlyView.
Extracellular vesicles (EVs) mediate intercellular communication in tumor immune microenvironments. However, their role in B‐cell malignancies remains poorly defined, owing to biological complexity and technical challenges in EV isolation and analysis.
Daniel Bachurski, Michael Hallek
wiley   +1 more source

The epithelial barrier theory proposes a comprehensive explanation for the origins of allergic and other chronic noncommunicable diseases

FEBS Letters, EarlyView.
Exposure to common noxious agents (1), including allergens, pollutants, and micro‐nanoplastics, can cause epithelial barrier damage (2) in our body's protective linings. This may trigger an immune response to our microbiome (3). The epithelial barrier theory explains how this process can lead to chronic noncommunicable diseases (4) affecting organs ...
Can Zeyneloglu, Huseyn Babayev, Ismail Ogulur, Sena Ardicli, Yagiz Pat, Duygu Yazici, Bingjie Zhao, Lihong Chang, Xiaoqing Liu, Paolo D'Avino, Manru Li, Ceren Biçer, Fatma Hacer Kurtoğlu Babayev, Raja Dhir, Kari C. Nadeau, Marie‐Charlotte Brüggen, Mubeccel Akdis, Cezmi A. Akdis   +17 more
wiley   +1 more source

Fluorescence antibunching microscopy

, 2011
Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of fluorescent ...
D. Oron, M. Kendall, O. Schwartz
core   +1 more source

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source