Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein [PDF]
Micromachines, 2021 Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid.
Puneet Jain +2 more
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Pressure and temperature dependence of fluorescence anisotropy of green fluorescent protein. [PDF]
RSC Adv, 2022 We have studied the effect of high hydrostatic pressure and temperature on the steady state fluorescence anisotropy of Green Fluorescent Protein (GFP). We find that the fluorescence anisotropy of GFP at a constant temperature decreases with increasing pressure.
Kaur H, Nguyen K, Kumar P.
europepmc +2 more sources
Live-cell microscopy or fluorescence anisotropy with budded baculoviruses—which way to go with measuring ligand binding to M4 muscarinic receptors? [PDF]
Open Biology, 2022 M4 muscarinic acetylcholine receptor is a G protein-coupled receptor (GPCR) that has been associated with alcohol and cocaine abuse, Alzheimer's disease, and schizophrenia which makes it an interesting drug target.
Maris-Johanna Tahk +10 more
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Fluorescence Anisotropy Sensor Comprising a Dual Hollow-Core Antiresonant Fiber Polarization Beam Splitter [PDF]
Sensors, 2020 Fluorescence anisotropy imaging and sensing is a widely recognized method for studying molecular orientation and mobility. However, introducing this technique to in vivo systems is a challenging task, especially when one considers multiphoton excitation ...
Hanna Izabela Stawska +1 more
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Triple Fluorescence Anisotropy Reporter Imaging in Living Cells [PDF]
Bio-Protocol, 2019 FRET-based genetically encoded biosensors incorporate two fluorescent proteins into their design to enable ratiometric biosensing of signaling activities in live cells.
Brian Ross +4 more
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Polarization modulation with optical lock-in detection reveals universal fluorescence anisotropy of subcellular structures in live cells [PDF]
Light: Science & Applications, 2022 The orientation of fluorophores can reveal crucial information about the structure and dynamics of their associated subcellular organelles. Despite significant progress in super-resolution, fluorescence polarization microscopy remains limited to unique ...
Meiling Guan +13 more
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π–π-Induced aggregation and single-crystal fluorescence anisotropy of 5,6,10b-triazaacephenanthrylene [PDF]
IUCrJ, 2018 The structural origin of absorption and fluorescence anisotropy of the single crystal of the π-conjugated heterocyclic system 5,6,10b-triazaacephenanthrylene, TAAP, is presented in this study.
Katarzyna Ostrowska +10 more
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Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association [PDF]
Nature Communications, 2020 Performing homo-FRET measurements in cells using a fluorescence microscope is challenging, especially when using high numerical aperture objective lenses.
Namrata Ojha +2 more
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Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock [PDF]
Methods and Protocols, 2019 Uniquely, the circadian clock of cyanobacteria can be reconstructed outside the complex milieu of live cells, greatly simplifying the investigation of a functioning biological chronometer. The core oscillator component is composed of only three proteins,
Joel Heisler +3 more
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A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin [PDF]
Nature Communications, 2018 Microtubule-targeting agents are used successfully as anticancer therapeutics. Here authors develop a fluorescence-anisotropy-based assay to identify and characterize ligands for the maytansine site of tubulin and provide crystal structures of identified
Grégory Menchon +10 more
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