Pressure and temperature dependence of fluorescence anisotropy of green fluorescent protein. [PDF]
RSC Adv, 2022 Effect of high hydrostatic pressure and temperature on the steady state fluorescence anisotropy of Green Fluorescent Protein (GFP).
Kaur H, Nguyen K, Kumar P.
europepmc +7 more sources
Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein [PDF]
Micromachines, 2021 Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid.
Puneet Jain +2 more
doaj +2 more sources
Live-cell microscopy or fluorescence anisotropy with budded baculoviruses—which way to go with measuring ligand binding to M4 muscarinic receptors? [PDF]
Open Biology, 2022 M4 muscarinic acetylcholine receptor is a G protein-coupled receptor (GPCR) that has been associated with alcohol and cocaine abuse, Alzheimer's disease, and schizophrenia which makes it an interesting drug target.
Maris-Johanna Tahk +10 more
doaj +2 more sources
Fluorescence Anisotropy Studies of Fluorescein
, 2021 Fluorescein anisotropy, which is a widely used technique to study the folding state of proteins or affinity of ligands, is used in the present work to study the temperature sensing of fluid in a microchannel, by adding fluorophore in the fluid. Fluorescein was used as a temperature probe, while glycerol-aq.
Puneet Jain +2 more
openalex +3 more sources
Droplet-based fluorescence anisotropy insulin immunoassay.
Anal MethodsFluorescence anisotropy immunoassay encapsulated in droplets for insulin detection on a microfluidic device.
Adeoye DI +5 more
europepmc +3 more sources
Fluorescence Anisotropy Sensor Comprising a Dual Hollow-Core Antiresonant Fiber Polarization Beam Splitter [PDF]
Sensors, 2020 Fluorescence anisotropy imaging and sensing is a widely recognized method for studying molecular orientation and mobility. However, introducing this technique to in vivo systems is a challenging task, especially when one considers multiphoton excitation ...
Hanna Izabela Stawska +1 more
doaj +2 more sources
Triple Fluorescence Anisotropy Reporter Imaging in Living Cells [PDF]
Bio-Protocol, 2019 FRET-based genetically encoded biosensors incorporate two fluorescent proteins into their design to enable ratiometric biosensing of signaling activities in live cells.
Brian Ross +4 more
doaj +2 more sources
Fluorescence anisotropy imaging in drug discovery. [PDF]
Adv Drug Deliv Rev, 2019 Non-invasive measurement of drug-target engagement can provide critical insights in the molecular pharmacology of small molecule drugs. Fluorescence polarization/fluorescence anisotropy measurements are commonly employed in protein/cell screening assays.
Vinegoni C +4 more
europepmc +4 more sources
Polarization modulation with optical lock-in detection reveals universal fluorescence anisotropy of subcellular structures in live cells [PDF]
Light: Science & Applications, 2022 The orientation of fluorophores can reveal crucial information about the structure and dynamics of their associated subcellular organelles. Despite significant progress in super-resolution, fluorescence polarization microscopy remains limited to unique ...
Meiling Guan +13 more
doaj +2 more sources
Reactivity of Olanzapine and Tricyclic Antidepressants on the Protective Effects of Trolox on Lipid Peroxidation Evaluated Using Fluorescence Anisotropy, Electron Paramagnetic Resonance Spectrometry, and Thermal Analysis. [PDF]
ACS Chem NeurosciHorizumi Y +5 more
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