Results 21 to 30 of about 69,474 (333)
Conformational Transitions Accompanying Oligomerization of Yeast Alcohol Oxidase, a Peroxisomal Flavoenzyme [PDF]
, 1999 Alcohol oxidase (AO) is a homo-octameric flavoenzyme which catalyzes methanol oxidation in methylotrophic yeasts. AO protein is synthesized in the cytosol and subsequently sorted to peroxisomes where the active enzyme is formed.
Boteva, R +6 more
core +7 more sources
Confocal fluorescence anisotropy and FRAP imaging of α-synuclein amyloid aggregates in living cells. [PDF]
PLoS ONE, 2011 We assessed the intracellular association states of the Parkinson's disease related protein α-synuclein (AS) in living cells by transfection with a functional recombinant mutant protein (AS-C4) bearing a tetracysteine tag binding the fluorogenic ...
M Julia Roberti +2 more
doaj +1 more source
Membranes, 2022 The antimicrobial peptide magainin 2 (M2) interacts with and induces structural damage in bacterial cell membranes. Although extensive biophysical studies have revealed the interaction mechanism between M2 and membranes, the mechanism of membrane ...
Munehiro Kumashiro +3 more
doaj +1 more source
Multiplexing Fluorescence Anisotropy Using Frequency Encoding [PDF]
Analytical Chemistry, 2016 In this report, a method to multiplex fluorescence anisotropy measurements is described using frequency encoding. As a demonstration of the method, simultaneous competitive immunoassays for insulin and glucagon were performed by measuring the ratio of bound and free Cy5-insulin and FITC-glucagon in the presence of their respective antibodies.
Adrian M, Schrell +2 more
openaire +2 more sources
Photophysics, Molecular Reorientation in Solution and X-Ray Structure of a New Fluorescent Probe 1,7-Diazaperylene [PDF]
, 1993 A new fluorescent molecule 1,7-diazaperylene (DP) has been investigated by means of time-resolved and steady-state polarized fluorescence spectroscopy, as well as X-ray spectroscopy.
Barkely +32 more
core +1 more source
Molecules, 2022 The influence of chitosan (CS) and amphiphilic polymers (AP: pluronic F108 and polyvinylpyrrolidone (PVP)) on the photocatalytic activity of rose bengal (RB) in a model reaction of tryptophan photo-oxidation in phosphate-buffered saline (PBS) was studied.
Anastasia S. Kuryanova +8 more
doaj +1 more source
Influence of Membrane Phase on the Optical Properties of DPH
Molecules, 2020 The fluorescent molecule diphenylhexatriene (DPH) has been often used in combination with fluorescence anisotropy measurements, yet little is known regarding the non-linear optical properties.
Silvio Osella +3 more
doaj +1 more source
Steady state fluorescence studies on lipase-vesicle interactions [PDF]
, 1994 The interaction of lipase from Candida cylindracea (CCL) with positively charged polymerizable surfactant vesicles was studied by the use of steady-state fluorescence techniques. The phase transition of vesicles composed of nonpolymerized and polymerized
Engbersen, Johan F.J. +3 more
core +3 more sources
Chemosensors, 2022 Biomacromolecules have been employed successfully as fluorescence anisotropy (FA) amplifiers for biosensing in reported studies. However, the sensitivities of the traditional biomacromolecule amplified FA strategies need to be improved because of the ...
Tianjin Xie +7 more
doaj +1 more source
Measuring F-actin properties in dendritic spines
Frontiers in Neuroanatomy, 2014 During the last decade, numerous studies have demonstrated that the actin cytoskeleton plays a pivotal role in the control of dendritic spine shape. Synaptic stimulation rapidly changes the actin dynamics and many actin regulators have been shown to play
Mikko eKoskinen, Pirta eHotulainen
doaj +1 more source