Results 291 to 300 of about 69,474 (333)
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Confocal fluorescence spectroscopy and anisotropy imaging system
Optics Letters, 2003We report the design and implementation of a laser scanning confocal fluorescence system with spectroscopy and anisotropy imaging capabilities. Confocal spectroscopy is achieved with a fiber pinhole that is inserted into and removed from the detection path as needed. Fluorescence anisotropy imaging is accomplished with a polarizing beam splitter placed
Chad E, Bigelow +2 more
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Fluorescence anisotropy: application in quantitative enzymatic determinations
Talanta, 2005In this work a method is presented for the enzymatic determination of glucose using fluorescence anisotropy. During the enzymatic reaction a change in the fluorescence anisotropy of the glucose oxidase (GOx) is produced; the reaction time at which this change appears (t(m)) depends on the glucose concentration.
Isabel, Sanz-Vicente +2 more
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Fluorescence anisotropy studies of molecularly imprinted polymers
Luminescence, 2005AbstractA molecularly imprinted polymer (MIP) is a biomimetic material that can be used as a biochemical sensing element. We studied the steady‐state and time‐resolved fluorescence and fluorescence anisotropy of anthracene‐imprinted polyurethane. We compared MIPs with imprinted analytes present, MIPs with the imprinted analytes extracted, MIPs with ...
Yin-Chu, Chen +3 more
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Measuring complexation by single-molecule fluorescence anisotropy
The Analyst, 2008The complexation of a fluorescent probe by a target protein was observed by single-molecule fluorescence anisotropy. Free and bound states, heterogeneities, and rare binding events can all be observed by this approach. Fluorophore-conjugated biotin was used to bind to NeutrAvidin as a proof-of-concept case.
Sean M, Burrows, Dimitri, Pappas
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Detection of Target Proteins by Fluorescence Anisotropy
Journal of Fluorescence, 2013Understanding molecular interactions is critical to understanding most biological mechanisms of cells and organisms. In the case of small molecule-protein interactions, many molecules have significant biological activity through interactions with unknown target proteins and by unknown modes of action. Identifying these target proteins is of significant
Lin, Wang +4 more
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Fluorescence Polarization Anisotropy to Measure RNA Dynamics
2009RNA requires helical motion to fold and carry out its function. As RNA helical motion occurs on the nanosecond timescale, the timescale probed by fluorescence dyes, fluorescence polarization anisotropy (FPA) is a simple, yet powerful, technique to study helical dynamics in RNA. With the recent development of several fluorescent base analogs that have a
Xuesong, Shi, Daniel, Herschlag
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Time-Resloved Fluorescence Anisotropy
2009Often in textbooks, complex methods such as fluorescence anisotropy and polarization are described using mathematical formulae. Although a formula can be a precise and concise means of conveying complex ideas between experts well trained in mathematics, the use of formulae can actually impede comprehension for a lay audience.
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Characterization of Chiral Interactions Using Fluorescence Anisotropy
Analytical Chemistry, 2006This work details a study whereby the characterization of chiral selectors and identification of optimal separation conditions is evaluated by steady-state fluorescence anisotropy measurements. Earlier studies in our laboratory have shown fluorescence anisotropy to be an effective tool in evaluating chiral recognition, and in this study, the ...
Kimaru, Irene +2 more
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Tubulin dimer dissociation detected by fluorescence anisotropy
Biochemistry, 1989We have demonstrated a concentration-dependent dissociation of bovine brain tubulin dimer covalently labeled with 5-[(4,6-dichlorotriazin-2-yl)amino]fluorescein (DTAF) or with fluorescein isothiocyanate (FITC) by fluorescence anisotropy and size-exclusion HPLC.
M R, Mejillano, R H, Himes
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Review of fluorescence anisotropy decay analysis by frequency-domain fluorescence spectroscopy
Journal of Fluorescence, 1993This didactic paper summarizes the mathematical expressions needed for analysis of fluorescence anisotropy decays from polarized frequency-domain fluorescence data. The observed values are the phase angle difference between the polarized components of the emission and the modulated anisotropy, which is the ratio of the polarized and amplitude-modulated
J R, Lakowicz +4 more
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