Results 121 to 130 of about 927,051 (305)

Combining antibody conjugates with cytotoxic and immune‐stimulating payloads maximizes anti‐cancer activity

Molecular Oncology, EarlyView.
Methods to improve antibody–drug conjugate (ADC) treatment durability in cancer therapy are needed. We utilized ADCs and immune‐stimulating antibody conjugates (ISACs), which are made from two non‐competitive antibodies, to enhance the entry of toxic payloads into cancer cells and deliver immunostimulatory agents into immune cells.
Tiexin Wang, Dong Jun Koo, Peter M. Tessier, Greg M. Thurber   +3 more
wiley   +1 more source

Multiple C-Terminal Tails within a Single \u3cem\u3eE. coli\u3c/em\u3e SSB Homotetramer Coordinate DNA Replication and Repair [PDF]

, 2013
Escherichia coli single-stranded DNA binding protein (SSB) plays essential roles in DNA replication, recombination and repair. SSB functions as a homotetramer with each subunit possessing a DNA binding domain (OB-fold) and an intrinsically disordered C ...
Antony, Edwin, Kozlov, Alexander G., Lohman, Timothy M., Manhart, Carol M., McHenry, Charles S., Nguyen, Binh, Weiland, Elizabeth, Yuan, Quan   +7 more
core   +1 more source

Polycations as Aptamer-Binding Modulators for Sensitive Fluorescence Anisotropy Assay of Aflatoxin B1

Sensors
Fluorescence induced by the excitation of a fluorophore with plane-polarized light has a different polarization depending on the size of the fluorophore-containing reagent and the rate of its rotation.
Alexey V. Samokhvalov, Alena A. Mironova, Sergei A. Eremin, Anatoly V. Zherdev, Boris B. Dzantiev   +4 more
doaj   +1 more source

Fluorescence lateral flow competitive protein binding assay for the assessment of serum folate concentrations.

PLoS ONE, 2019
Folate is a micronutrient required for the production of new cells, making it a key factor in early fetal development and ensuring normal growth and maintenance of health.
Elizabeth G Rey, Julia L Finkelstein, David Erickson   +2 more
doaj   +1 more source

Dammarenediol II enhances etoposide‐induced apoptosis by targeting O‐GlcNAc transferase and Akt/GSK3β/mTOR signaling in liver cancer

Molecular Oncology, EarlyView.
Etoposide induces DNA damage, activating p53‐dependent apoptosis via caspase‐3/7, which cleaves PARP1. Dammarenediol II enhances this apoptotic pathway by suppressing O‐GlcNAc transferase activity, further decreasing O‐GlcNAcylation. The reduction in O‐GlcNAc levels boosts p53‐driven apoptosis and influences the Akt/GSK3β/mTOR signaling pathway ...
Jaehoon Lee, Byung‐Cheol Han, Gi‐Bang Koo, Jihye Park, Mijin Kwon, Young Bin Park, Jae‐Mun Choi, Seung‐Ho Lee, Sangho Roh   +8 more
wiley   +1 more source

Tb3+-nucleic acid probe-based label-free and rapid detection of mercury pollution in food

Food Science and Human Wellness
Mercury is a threatening pollutant in food, herein, we developed a Tb3+-nucleic acid probe-based label-free assay for mix-and-read, rapid detection of mercury pollution.
Xuhan Xia, Chenxi Zhou, Yulin Zhu, Yi Dong, Qiang He, Mohammad Rizwan Khan, Yuanlong Chi, Rosa Busquets, Ruijie Deng, Yao Ren   +9 more
doaj   +1 more source

Idiosyncrasies of thermofluorimetric aptamer binding assays

BioTechniques, 2019
To explore thermofluorimetric analysis (TFA) in detail, we compared two related aptamers. The first, LINN2, is a DNA aptamer previously selected against EGFR recombinant protein. In this work we selected a second aptamer, KM4, against EGFR-overexpressing
Tulsi Ram Damase, Peter B Allen
doaj   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Heteroreceptor complexes and their allosteric receptor-receptor interactions in the central nervous system. Focus on examples from Dopamine D2 and Serotonin 5-HT1a receptors [PDF]

, 2018
GPCR interacting proteins (specially β- arrestin) and their receptor-protein interactions are also covered but their interactions with the allosteric receptor-receptor interactions in heteroreceptor complexes remain to be elucidated.
Corrales Ferro, Fidel
core   +1 more source

A dual fluorescence-based reporter assay for real-time determination of siRNA- and antisense oligonucleotide-mediated knockdown

Molecular Therapy: Nucleic Acids
In recent years, many tools have been developed for targeted treatments of cancer or rare diseases including nucleic acid (NA)-based therapeutics. One of the developmental caveats is the assessment of their functionality in vivo. We therefore developed a
Felix Heß, Margarete Odenthal, Elena Wasserburger-Zichel, Christina Grimm, Michal R. Schweiger   +4 more
doaj   +1 more source