Results 231 to 240 of about 319,369 (290)
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Fluorescence Lifetime Correlation Spectroscopy
Journal of Fluorescence, 2006This article explains the basic principles of FLCS, a genuine fusion of Time-Correlated Single Photon Counting (TCSPC) and Fluorescence Correlation Spectroscopy (FCS), using common terms and minimum mathematics. The usefulness of the method is demonstrated on simple FCS experiments.
Peter, Kapusta +4 more
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Fluorescence Correlation Spectroscopy
2007Fluorescence correlation spectroscopy (FCS) is a technique that allows for an extremely sensitive determination of molecular diffusion properties, down to the level of single molecules. It thus provides an attractive alternative to FRAP, requiring much less laser power and lower concentrations of fluorophores.
Kirsten, Bacia, Petra, Schwille
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Noise on Fluorescence Correlation Spectroscopy
Journal of Colloid and Interface Science, 2001The time dependence of the noise and the signal-to-noise (SN) ratio of the fluorescence correlation spectroscopy (FCS) autocorrelation function is obtained from replica measurements of standard dextran solutions. The noise dependence on the delay time is fitted by a hyperbolic function with two fitting parameters.
Starchev K, Ricka J, Buffle J
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Inverse-Fluorescence Correlation Spectroscopy
Analytical Chemistry, 2009An alternative version of fluorescence correlation spectroscopy is presented, where the signal from a medium surrounding the particles of interest is analyzed, as opposed to a signal from the particles themselves. This allows for analysis of unlabeled particles and potentially of biomolecules.
Stefan, Wennmalm +3 more
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Microrheology with Fluorescence Correlation Spectroscopy
Langmuir, 2009We show that fluorescence correlation spectroscopy (FCS) using a commercial spectrometer can be applied to passive microrheological (MR) experiments. The method probes the local rheological properties of materials on length scales of the focus dimension of the confocal microscope.
Rathgeber, S. +4 more
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Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy, 2022
In this review, the comprehensive summary of two-dimensional correlation spectroscopy (2D-COS) for the last two years is covered. The remarkable applications of 2D-COS in diverse fields using many types of probes and perturbations for the last two years ...
Yeonju Park, Sila Jin, I. Noda, Y. Jung
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In this review, the comprehensive summary of two-dimensional correlation spectroscopy (2D-COS) for the last two years is covered. The remarkable applications of 2D-COS in diverse fields using many types of probes and perturbations for the last two years ...
Yeonju Park, Sila Jin, I. Noda, Y. Jung
semanticscholar +1 more source
Recent advances in fluorescence correlation spectroscopy
Current Opinion in Structural Biology, 2002Fluorescence correlation spectroscopy is a method in which fluctuations in the fluorescence arising from a very small sample volume are correlated to obtain information about the processes giving rise to the fluctuations. Recent progress has been made in methodologies such as two-photon excitation, photon counting histogram analysis, cross-correlation,
Nancy L Thompson
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On the statistics of fluorescence correlation spectroscopy
Biophysical Chemistry, 1990I present a detailed statistical analysis of fluorescence correlation spectroscopy (FCS) which is a natural extension of an early work. This analysis more realistically takes account of the following issues. (1) A spatial Gaussian laser excitation of fluorescence, (2) the effect of a small number of fluorescent molecules in the observation volume, (3 ...
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Applications of Fluorescence Correlation Spectroscopy
Quarterly Reviews of Biophysics, 1976The preceding paper by Douglas Magde has recounted the basic principles of Fluorescence Correlation Spectroscopy (FCS) as originally described (see Magde, Elson & Webb, 1972; Elson & Magde, 1974; Magde, Elson & Webb, 1974 Elson & Webb, 1975; referred to collectively as MEW), and has described the first application to chemical kinetics ...
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Recent applications of fluorescence correlation spectroscopy in live systems
FEBS Letters, 2014 Fluorescence correlation spectroscopy (FCS) is a widely used technique in biophysics and has helped address many questions in the life sciences. It provides important advantages compared to other fluorescence and biophysical methods.
Thorsten Wohland, Radek Machan
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