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Fluorescence Correlation Spectroscopy
2014Fluorescence fluctuation spectroscopy techniques allow the quantification of fluorescent molecules present at the nanomolar concentration level. After a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecular interaction of two signaling proteins inside the ...
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Fluorescence Correlation Spectroscopy
2006An experimental application of total internal reflection with fluorescence correlation spectroscopy (TIR/FCS) is presented. TIR/FCS is a new technique for measuring the binding and unbinding rates and surface diffusion coefficient of fluorescent-labeled solute molecules in equilibrium at a surface.
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Advanced fluorescence correlation spectroscopy for studying biomolecular conformation.
Current Opinion in Structural Biology, 2021A. Ghosh, J. Enderlein
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Inverse-Fluorescence Cross-Correlation Spectroscopy
Analytical Chemistry, 2010Inverse-fluorescence correlation spectroscopy (iFCS) was recently introduced as an alternative version of FCS that does not require labeling of the analyzed particles or biomolecules. In iFCS, the signal from a medium surrounding the particles is analyzed, as opposed to a signal from the studied particles themselves.
Stefan, Wennmalm, Jerker, Widengren
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Fluorescence correlation spectroscopy in living cells
Nature Methods, 2007Fluorescence correlation spectroscopy (FCS) is an ideal analytical tool for studying concentrations, propagation, interactions and internal dynamics of molecules at nanomolar concentrations in living cells. FCS analyzes minute fluorescence-intensity fluctuations about the equilibrium of a small ensemble (
Sally A, Kim +2 more
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Biomedical Optics and 3-D Imaging, 2010
Practical limits of Fluorescence-Lifetime Correlation Spectroscopy (FLCS) were explored. It shown that FLCS yields correct concentration values down to the picomolar range and that different signal components can be separated in a single detector setup.
Steffen Rüttinger +4 more
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Practical limits of Fluorescence-Lifetime Correlation Spectroscopy (FLCS) were explored. It shown that FLCS yields correct concentration values down to the picomolar range and that different signal components can be separated in a single detector setup.
Steffen Rüttinger +4 more
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Fluorescence Correlation and Cross-Correlation Spectroscopy in Zebrafish
2018There has been increasing interest in biophysical studies on live organisms to gain better insights into physiologically relevant biological events at the molecular level. Zebrafish (Danio rerio) is a viable vertebrate model to study such events due to its genetic and evolutionary similarities to humans, amenability to less invasive fluorescence ...
Xue Wen, Ng +2 more
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Spatial fluorescence cross-correlation spectroscopy
Applied Optics, 2006We present an alternative method for diffusion measurements of fluorescent species in solution by use of confocal microscopy and fluorescence correlation spectroscopy techniques. It consists of making a time and spatial dual correlation in which one detects the fluorescence signals from two nearby separate confocal volumes and cross correlates them. To
Rodolphe, Jaffiol +3 more
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New Perspectives of Fluorescence Correlation Spectroscopy
Journal of Fluorescence, 1999The principle of fluorescence correlation spectroscopy is outlined. The technique has been applied to a mutant of the well-known green fluorescent protein. A comparative study has been made with time-resolved fluorescence anisotropy. The latter experiment shows that the fluorophore is rigidly bound inside the protein matrix follows the rotation of the ...
Hink, M.A., Visser, A.J.W.G.
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Detection of H2O2 by Fluorescence Correlation Spectroscopy
2013Fluorescence correlation spectroscopy (FCS) is a technique in which measurement of fluorescence intensity fluctuations is used to clarify dynamic molecular interactions within a very small space in a solution containing a small number of fluorescent molecules. The FCS-based analysis gives the average number and average diffusion time of the fluorescent
Etsuro, Ito +5 more
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