Results 51 to 60 of about 74,021 (249)

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Estimating Poly(N-isopropylacrylamide) size in solution below the LCST using fluorescence correlation spectroscopy with non-covalent bound fluorophores

Polymer Testing
Accurate size measurements of nanosized polymer chains in dilute solutions is important for understanding polymer behavior, however, these measurements can be challenging to implement accurately, and are technique dependent.
Camila van Zanten, Alan G. Ryder
doaj   +1 more source

Monitoring drug nanocarriers in human blood by near-infrared fluorescence correlation spectroscopy

Nature Communications, 2018
While nanocarrier-based drug delivery is a promising therapeutic approach, in situ characterization of drug nanocarriers in blood remains difficult.
Inka Negwer, Andreas Best, Meike Schinnerer, Olga Schäfer, Leon Capeloa, Manfred Wagner, Manfred Schmidt, Volker Mailänder, Mark Helm, Matthias Barz, Hans-Jürgen Butt, Kaloian Koynov   +11 more
doaj   +1 more source

Direct Vpr-Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging

Retrovirology, 2008
Background The human immunodeficiency virus type 1 (HIV-1) encodes several regulatory proteins, notably Vpr which influences the survival of the infected cells by causing a G2/M arrest and apoptosis.
Mély Yves, Darlix Jean-Luc, Bouaziz Serge, Morellet Nelly, Cabanne Charlotte, Muriaux Delphine, Schaub Emmanuel, Clamme Jean-Pierre, Didier Pascal, Fritz Joëlle V, de Rocquigny Hugues   +10 more
doaj   +1 more source

PAK1 activation drives divergent resistance mechanisms to aromatase inhibition and tamoxifen in a luminal: A breast cancer model

Molecular Oncology, EarlyView.
Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller, Janina Müller, Efstathios‐Iason Vlachavas, Lukas Beumers, Lena Fleischhacker, Sara Burmester, Angelika Wörner, Sabine Karolus, Dominic Helm, Cindy Körner, Stefan Wiemann   +10 more
wiley   +1 more source

Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models

Biomolecules, 2020
Alkylphospholipids are a novel class of antineoplastic drugs showing remarkable therapeutic potential. Among them, erufosine (EPC3) is a promising drug for the treatment of several types of tumors.
Rumiana Tzoneva, Tihomira Stoyanova, Annett Petrich, Desislava Popova, Veselina Uzunova, Albena Momchilova, Salvatore Chiantia   +6 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Fluorescence correlation spectroscopy for the characterization of membranes : A short review [PDF]

Songklanakarin Journal of Science and Technology (SJST), 2002
Fluorescence correlation spectroscopy (FCS) is a highly sensitive fluorescence microscopy technique that can be used to probe a wide range of biophysical processes including diffusion, Ligand-receptor binding and molecular aggregation on artificial and ...
Hwang, L.C., Thorsten Wohland
doaj  

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Multi-confocal fluorescence correlation spectroscopy [PDF]

Frontiers in Bioscience, 2011
We report a multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique that combines a Spatial Light Modulator (SLM), with an Electron Multiplying-CCD camera (EM-CCD). The SLM is used to produce a series of laser spots, while the pixels of the EM-CCD play the roles of virtual pinholes.
Galland, Rémi, Gao, Jie, Kloster, Meike, Herbomel, Gaetan, Destaing, Olivier, Balland, Martial, Souchier, Catherine, Usson, Yves, Derouard, Jacques, Wang, Irène, Delon, Antoine   +10 more
openaire   +4 more sources