Results 31 to 40 of about 319,369 (290)

Fluorescence spectroscopy for clinical transplantation liver grafts monitoring: possibilities offered by 408 nm excitation

open access: yesActa Cirúrgica Brasileira, 2022
Purpose: Fluorescence spectroscopy techniques have been investigated aiming to reduce the invasiveness of methods for investigation of tissue. In transplantation procedures, it may offer the possibility of a complementary technique for the monitoring of ...
José Dirceu Vollet-Filho   +5 more
doaj   +1 more source

Perspective: Chain dynamics of unfolded and intrinsically disordered proteins from nanosecond fluorescence correlation spectroscopy combined with single-molecule FRET.

open access: yesJournal of Chemical Physics, 2018
The dynamics of unfolded proteins are important both for the process of protein folding and for the behavior of intrinsically disordered proteins. However, methods for investigating the global chain dynamics of these structurally diverse systems have ...
B. Schuler
semanticscholar   +1 more source

Super-resolution fluorescence imaging and correlation spectroscopy: Principles and examples of application [PDF]

open access: yesJournal of the Serbian Chemical Society, 2013
Self-organization of cell-surface receptors in structurally distinct domains in the plasma membrane is of vital interest for correct cellular signaling. However, this dynamic process is difficult to study in cells with sufficiently high temporal
Jovanović-Talisman Tijana   +1 more
doaj   +1 more source

Spot variation fluorescence correlation spectroscopy by data post-processing

open access: yesScientific Reports, 2017
Spot variation fluorescence correlation spectroscopy (SV-FCS) is a variant of the FCS techniques which may give useful information about the structural organisation of the medium in which the diffusion takes place.
S. M. J. Khadem   +3 more
doaj   +1 more source

Fluorescence Correlation Spectroscopy to find the critical balance between extracellular association and intracellular dissociation of mRNA complexes.

open access: yesActa Biomaterialia, 2018
Fluorescence Correlation Spectroscopy (FCS) is a promising tool to study interactions on a single molecule level. The diffusion of fluorescent molecules in and out of the excitation volume of a confocal microscope leads to the fluorescence fluctuations ...
Heyang Zhang, S. D. De Smedt, K. Remaut
semanticscholar   +1 more source

Multicolor fluorescence fluctuation spectroscopy in living cells via spectral detection

open access: yeseLife, 2021
Signaling pathways in biological systems rely on specific interactions between multiple biomolecules. Fluorescence fluctuation spectroscopy provides a powerful toolbox to quantify such interactions directly in living cells.
Valentin Dunsing   +2 more
doaj   +1 more source

Quantifying Reversible Surface Binding via Surface-Integrated Fluorescence Correlation Spectroscopy

open access: yesNano letters (Print), 2018
We present a simple and versatile single-molecule-based method for the accurate determination of binding rates to surfaces or surface bound receptors.
Jonas Mücksch   +5 more
semanticscholar   +1 more source

Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy

open access: yeseLife, 2020
Development and homeostasis of multicellular organisms is largely controlled by complex cell-cell signaling networks that rely on specific binding of secreted ligands to cell surface receptors.
Antonia Franziska Eckert   +7 more
doaj   +1 more source

Fluorescence Correlation Spectroscopy Combined with Multiphoton Laser Scanning Microscopy—A Practical Guideline

open access: yesApplied Sciences, 2021
Multiphoton laser scanning microscopy (MPM) has opened up an optical window into biological tissues; however, imaging is primarily qualitative. Cell morphology and tissue architectures can be clearly visualized but quantitative analysis of actual ...
Jeemol James   +2 more
doaj   +1 more source

Measurement of diffusion in articular cartilage using fluorescence correlation spectroscopy

open access: yesBMC Biotechnology, 2011
Background Fluorescence correlation spectroscopy (FCS) provides information about translational diffusion of fluorescent molecules in tiny detection volumes at the single-molecule level.
Ushida Kiminori   +3 more
doaj   +1 more source

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