Results 11 to 20 of about 74,021 (249)

Fluorescence correlation spectroscopy and fluorescence cross‐correlation spectroscopy [PDF]

open access: yesWIREs Systems Biology and Medicine, 2009
AbstractThis article focuses on methods based on fluctuation correlation spectroscopy to determine the formation of protein complexes in living cells. We present the principles of the fluctuation method applied to cells. We discuss the novelty and the promises of this approach. The emphasis is in the discussion of the underlying statistical assumptions
Digman, Michelle A, Gratton, Enrico
openaire   +4 more sources

Research Progress of Fluorescence Spectrum Analysis Technology in Food Detection

open access: yesShipin gongye ke-ji, 2022
As a testing technology for food, fluorescence spectrometry has the advantages of convenient, sensitive and efficient detection of complex and trace components in food.
Xin SHI   +4 more
doaj   +1 more source

Fluorescence Correlation Spectroscopy [PDF]

open access: yes, 2011
Fluorescence correlation spectroscopy (FCS), implemented in microscopy, relies on performing an autocorrelation of the time fluctuating intensity arising from individual molecules diffusing through a confocal volume. It allows us to investigate a large variety of dynamic processes and to quantify photophysical, photochemical, interaction, diffusion ...
Ferrand, Patrick   +2 more
openaire   +4 more sources

Measurement of the Concentration and the Brightness for Samples Containing Multiple Molecules with Different Brightness Using Fluorescence Correlation Spectroscopy

open access: yesApplied Sciences, 2021
In this study, the concentration and brightness measured by fluorescence correlation spectroscopy (FCS) in samples containing multiple species with different brightness levels was demonstrated.
Johtaro Yamamoto, Akira Sasaki
doaj   +1 more source

Ultrabright Green-Emitting Nanoemulsions Based on Natural Lipids-BODIPY Conjugates

open access: yesNanomaterials, 2021
Nanoemulsions (NEs) are water-dispersed oil droplets that constitute stealth biocompatible nanomaterials. NEs can reach an impressive degree of fluorescent brightness owing to their oily core that can encapsulate a large number of fluorophores on the ...
Xinyue Wang   +4 more
doaj   +1 more source

Fluorescence Correlation Spectroscopy Measurement Based on Fiber Optics for Biological Materials

open access: yesApplied Sciences, 2021
A robust fluorescence correlation spectroscopy system called fiber-optic based fluorescence correlation spectroscopy (FB-FCS) was developed; this system enables the measurement of diffusion dynamics and concentration of fluorescent molecules based on the
Johtaro Yamamoto, Akira Sasaki
doaj   +1 more source

Monitoring Molecular Properties of a Fluorescence Light-Up Aptamer Using Fluorescence Cross-Correlation Spectroscopy

open access: yesApplied Sciences, 2022
Fluorescence light-up aptamers (FLAPs) are tools for RNA imaging, wherein the RNA of interest is appended with a FLAP sequence that can bind to a corresponding small-molecule fluorogen and enhance its fluorescence.
Yuichi Furuhata, Akira Sasaki
doaj   +1 more source

Supercritical Angle Fluorescence Correlation Spectroscopy [PDF]

open access: yesBiophysical Journal, 2008
We explore the potential of a supercritical angle (SA) objective for fluorescence correlation spectroscopy (FCS). This novel microscope objective combines tight focusing by an aspheric lens with strong axial confinement of supercritical angle fluorescence collection by a parabolic mirror lens, resulting in a small detection volume.
Ries, J   +3 more
openaire   +3 more sources

Adaptive Optics for Fluorescence Correlation Spectroscopy [PDF]

open access: yesBiophysical Journal, 2011
Fluorescence Correlation Spectroscopy (FCS) yields measurement parameters (number of molecules, diffusion time) that characterize the concentration and kinetics of fluorescent molecules within a supposedly known observation volume. Absolute derivation of concentrations and diffusion constants therefore requires preliminary calibrations of the confocal ...
Leroux, Charles Edouard   +3 more
openaire   +4 more sources

Sucrose monoester micelles size determined by Fluorescence Correlation Spectroscopy (FCS). [PDF]

open access: yesPLoS ONE, 2011
One of the several uses of sucrose detergents, as well as other micelle forming detergents, is the solubilization of different membrane proteins.
Susana A Sanchez   +4 more
doaj   +1 more source

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