Results 21 to 30 of about 319,369 (290)

Adaptive Optics for Fluorescence Correlation Spectroscopy [PDF]

Biophysical Journal, 2011
Fluorescence Correlation Spectroscopy (FCS) yields measurement parameters (number of molecules, diffusion time) that characterize the concentration and kinetics of fluorescent molecules within a supposedly known observation volume. Absolute derivation of concentrations and diffusion constants therefore requires preliminary calibrations of the confocal ...
Leroux, Charles Edouard, Wang, Irène, Derouard, Jacques, Delon, Antoine   +3 more
openaire   +4 more sources

The Standard Deviation in Fluorescence Correlation Spectroscopy [PDF]

Biophysical Journal, 2001
The standard deviation (SD) in fluorescence correlation spectroscopy (FCS) has been mostly neglected in applications. However, the knowledge of the correct SD is necessary for an accurate data evaluation, especially when fitting theoretical models to experimental data. In this work, an algorithm is presented that considers the essential features of FCS.
Wohland, Thorsten, Rigler, Rudolf, Vogel, Horst   +2 more
openaire   +3 more sources

Systematic characterization of extracellular vesicle sorting domains and quantification at the single molecule – single vesicle level by fluorescence correlation spectroscopy and single particle imaging

Journal of Extracellular Vesicles, 2019
Extracellular vesicles (EV) convey biological information by transmitting macromolecules between cells and tissues and are of great promise as pharmaceutical nanocarriers, and as therapeutic per se. Strategies for customizing the EV surface and cargo are
Giulia Corso, Wolf Heusermann, Dominic Trojer, Andre Görgens, Emmanuelle Steib, J. Voshol, A. Graff, C. Genoud, Y. Lee, Justin Hean, J. Nordin, O. Wiklander, S. El Andaloussi, Nicole C Meisner-Kober   +13 more
semanticscholar   +1 more source

Sucrose monoester micelles size determined by Fluorescence Correlation Spectroscopy (FCS). [PDF]

PLoS ONE, 2011
One of the several uses of sucrose detergents, as well as other micelle forming detergents, is the solubilization of different membrane proteins.
Susana A Sanchez, Enrico Gratton, Antonio L Zanocco, Else Lemp, German Gunther   +4 more
doaj   +1 more source

Dissolved organic matter and its correlation with phytoplankton abundance for monitoring surface water quality [PDF]

Global Journal of Environmental Science and Management, 2022
BACKGROUND AND OBJECTIVES: Dissolved organic matter has a fundamental role in supporting phytoplankton abundance and growth in aquatic environments.
O. Cahyonugroho, S. Hariyanto, G. Supriyanto   +2 more
doaj   +1 more source

Wnt3 Is Lipidated at Conserved Cysteine and Serine Residues in Zebrafish Neural Tissue

Frontiers in Cell and Developmental Biology, 2021
Wnt proteins are a family of hydrophobic cysteine-rich secreted glycoproteins that regulate a gamut of physiological processes involved in embryonic development and tissue homeostasis.
Divya Dhasmana, Sapthaswaran Veerapathiran, Yagmur Azbazdar, Yagmur Azbazdar, Ashwin Venkata Subba Nelanuthala, Cathleen Teh, Gunes Ozhan, Gunes Ozhan, Thorsten Wohland, Thorsten Wohland   +9 more
doaj   +1 more source

The correlation analysis of TOC, CODCr and fluorescence characteristics in MPR [PDF]

E3S Web of Conferences, 2020
Water samples from different biochemical reaction time in one reactor period in Micro-Pressure Inner-Loop Bioreactor (MPR) were measured respectively with adopt Excitation-emission matrix (EEM) fluorescence spectroscopy, Total organic carbon (TOC) and ...
Tian Xi, Liu Ying, Zhao Chunling, Ji Xiaona, Ren Zhiming, Bian Dejun   +5 more
doaj   +1 more source

Nano-antenna enhanced two-focus fluorescence correlation spectroscopy

Scientific Reports, 2017
We propose two-focus fluorescence correlation spectroscopy (2fFCS) on basis of plasmonic nanoantennas that provide distinct hot spots that are individually addressable through polarization, yet lie within a single diffraction limited microscope focus ...
Lutz Langguth, Agata Szuba, Sander A. Mann, Erik C. Garnett, Gijsje H. Koenderink, A. Femius Koenderink   +5 more
doaj   +1 more source

Diffusion Measurements of Swimming Enzymes with Fluorescence Correlation Spectroscopy.

Accounts of Chemical Research, 2018
Self-propelled chemical motors are chemically powered micro- or nanosized swimmers. The energy required for these motors' active motion derives from catalytic chemical reactions and the transformation of a fuel dissolved in the solution.
Jan-Philipp Günther, M. Börsch, P. Fischer   +2 more
semanticscholar   +1 more source

Fluorescence correlation spectroscopy as a tool for the study of the intracellular dynamics and biological fate of protein corona.

Biophysical Chemistry, 2019
In biological fluids, nanoparticles (NPs) are in contact with proteins and other biomolecules. Proteins adsorb to NPs and form a coating called a protein corona (PC). The PC is known to greatly affect the interaction of NPs with biological systems.
Marta Martínez-Moro, Désire Di Silvio, S. Moya   +2 more
semanticscholar   +1 more source