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Using fluorescence correlation spectroscopy (FCS) for IFN-g detection: A preliminary study
Journal of Immunological Methods, 2014Nowadays, enzyme-linked immunosorbent assay (ELISA) based detection of Mycobacterium tuberculosis (M. tuberculosis) antigen triggered interferon-gamma (IFN-g) secretion by blood T cells displays an improved diagnostic value for M. tuberculosis infection. Applications of fluorescence correlation spectroscopy (FCS) have been explored in various subfields
Erwin Gaubitzer
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FCS videos: Fluorescence correlation spectroscopy in space and time
Biochimica Et Biophysica Acta - General SubjectsFluorescence Correlation Spectroscopy (FCS), invented more than 50 years ago is a widely used tool providing information on molecular processes in a variety of samples from materials to life sciences. In the last two decades FCS was multiplexed and ultimately made into an imaging technique that provided maps of molecular parameters over whole sample ...
Thorsten Wohland, Shambhavi Pandey
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Advanced FCS: An Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual-Focus FCS
Springer Series on Fluorescence, 2014This chapter focuses on two advanced fluorescence correlation spectroscopy (FCS) methods; fluorescence lifetime correlation spectroscopy (FLCS) and dual-focus FCS (2fFCS). We decided to put our focus on a detailed discussion of these two – and in our eyes well-merited – advanced methods, rather than giving an overview over the broad variety of advanced
Thomas Dertinger
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Focal volume characterization using multiphoton fluorescence correlation spectroscopy (MP-FCS)
SPIE Proceedings, 2004Fluorescence correlation spectroscopy(FCS) normally employs measured fluctuations in molecular fluorescence from within a defined observation volume created by a laser focus to extract information about dynamic processes associated with those molecules.
M Neal Waxham, Vijay Iyer
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Fluorescence correlation spectroscopy as tool for high-content-screening in yeast (HCS-FCS)
Proceedings of SPIE, 2011To measure protein interactions, diffusion properties, and local concentrations in single cells, Fluorescence Correlation Spectroscopy (FCS) is a well-established and widely accepted method. However, measurements can take a long time and are laborious. Therefore investigations are typically limited to tens or a few hundred cells.
Jay Unruh, Christopher Wood
exaly +2 more sources
Tailor-Made Dyes for Fluorescence Correlation Spectroscopy (FCS)
Biological Chemistry, 2001Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes.
P, Czerney +5 more
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Diffusion and Binding Properties Investigated by Fluorescence Correlation Spectroscopy (FCS)
Current Pharmaceutical Biotechnology, 2005During the last years, Fluorescence Correlation Spectroscopy (FCS) has proven to be a powerful tool for basic research in many applications. The combination of a minimal detection volume in the femtoliter range coupled with very high sensitivity extends the possibilities to design sensitive homogeneous tests.
D, Grünwald +3 more
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A new approach for fluorescence correlation spectroscopy (FCS) based immunoassays
Journal of Biotechnology, 2004Fluorescence correlation spectroscopy (FCS) is a powerful technique for measuring physicochemical properties, such as concentration and diffusion constant, of bio-molecules in complex mixtures. Although, as such, FCS is well suited for development of homogeneous immunoassays, a major obstacle lies in the relatively high molecular weight of antibodies ...
Oleg A, Mayboroda +4 more
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2001
Fluorescence correlation spectroscopy (FCS) has traditionally been applied to well-defined in vitro systems, in which only the olecules of interest emit fluorescence. However, the ability to determine absolute molecule numbers, the inherent high sensitivity, and the possibility of measuring molecular diffusion and reactions in minute detection volumes ...
Brock, R., Jovin, T.
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Fluorescence correlation spectroscopy (FCS) has traditionally been applied to well-defined in vitro systems, in which only the olecules of interest emit fluorescence. However, the ability to determine absolute molecule numbers, the inherent high sensitivity, and the possibility of measuring molecular diffusion and reactions in minute detection volumes ...
Brock, R., Jovin, T.
openaire +2 more sources
Principles and Applications of Fluorescence Correlation Spectroscopy (FCS)
2010Fluorescence correlation spectroscopy (FCS) is one of the various modern optical techniques that allow access to single fluorescently labeled biomolecules in aqueous solution. In contrast to other fluorescence measurements, however, the parameter of primary interest is not the emission intensity itself, but rather spontaneous intensity fluctuations ...
Petra Schwille, Jonas Ries
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