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Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS)
2013Fluorescence correlation spectroscopy (FCS) is a powerful method to investigate molecular interactions based on the variation of diffusion properties at the single-molecule level. This technique allows studying quantitatively the interaction of fluorescently labeled proteins/peptides with lipid vesicles. Here, we describe how to acquire and analyze FCS
Ana M, Melo +2 more
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Cellular and molecular biology (Noisy-le-Grand, France), 1998
Confocal fluorescence correlation spectroscopy (FCS) and other confocal spectroscopic techniques are ideally suited for the analysis of molecular interactions at the subcellular level. However, one requires exact positioning in three dimensions within the cell.
Brock, R., Jovin, T.
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Confocal fluorescence correlation spectroscopy (FCS) and other confocal spectroscopic techniques are ideally suited for the analysis of molecular interactions at the subcellular level. However, one requires exact positioning in three dimensions within the cell.
Brock, R., Jovin, T.
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FLUORESCENCE CORRELATION SPECTROSCOPY (FCS) – 50 YEARS AND BEYOND
Physical Chemistry 2024 : proceedings Vol. 1This lecture will present the theoretical background and historical milestones of fluorescence correlation spectroscopy (FCS), reflecting on key scientific contributions and the transformative impact of this technique on modern biomedical research.
Sho Oasa +4 more
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Journal of Receptors and Signal Transduction, 1997
Fluorescence Correlation Spectroscopy (FCS) a new analytical technology, allows binding properties to be determined very accurately in biological assays at the level of single molecules. At concentrations of > or = 10(-12) M, binding constants, on/off-rates, and even reaction/enzyme kinetics can be determined in real-time, and in sample volumes as low ...
S, Sterrer, K, Henco
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Fluorescence Correlation Spectroscopy (FCS) a new analytical technology, allows binding properties to be determined very accurately in biological assays at the level of single molecules. At concentrations of > or = 10(-12) M, binding constants, on/off-rates, and even reaction/enzyme kinetics can be determined in real-time, and in sample volumes as low ...
S, Sterrer, K, Henco
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2013
Fluorescence correlation spectroscopy (FCS) can add dynamic molecular information to images of live cells. For example, a confocal laser scanning microscope (CLSM) equipped with an accessory FCS unit provides the possibility to first image the spatial distribution of a fluorescent protein before probing its mobility within defined regions of interest ...
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Fluorescence correlation spectroscopy (FCS) can add dynamic molecular information to images of live cells. For example, a confocal laser scanning microscope (CLSM) equipped with an accessory FCS unit provides the possibility to first image the spatial distribution of a fluorescent protein before probing its mobility within defined regions of interest ...
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Fluorescence Correlation Spectroscopy (FCS) Analysis of Human Red Blood Cell System
Optical Review, 2003Recent studies have revealed the importance of the lipid micro domain for signal transduction in cell membrane. To analyze the biophysical properties of the lipid micro domain at the single molecule level, we measured the diffusion of fluorescence probe in human red blood cell (RBC) membrane using fluorescence correlation spectroscopy (FCS).
Yasuo Takahashi +3 more
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2009
Carbohydrate-protein interaction is very important in understanding the function of sugar chains. The immobilization or fluorescence modification of oligosaccharides was investigated to understand the biological interaction between various species and oligosaccharides.
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Carbohydrate-protein interaction is very important in understanding the function of sugar chains. The immobilization or fluorescence modification of oligosaccharides was investigated to understand the biological interaction between various species and oligosaccharides.
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Investigation of EGF interaction with EGFR variants by fluorescence correlation spectroscopy (FCS)
Regulatory Peptides, 2000Jagdish Rao +4 more
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