Results 11 to 20 of about 7,002 (182)
Applied Sciences, 2022 Fluorescence light-up aptamers (FLAPs) are tools for RNA imaging, wherein the RNA of interest is appended with a FLAP sequence that can bind to a corresponding small-molecule fluorogen and enhance its fluorescence.
Yuichi Furuhata, Akira Sasaki
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Supramolecular association studied by Fluorescence correlation spectroscopy
Frontiers in Chemistry, 2022 A comprehensive description of a supramolecular system involves a full understanding of its thermodynamic and dynamic properties, as well as detailed knowledge of its structure. Fluorescence Correlation Spectroscopy (FCS) constitutes a powerful technique
Mercedes Novo, Wajih Al-Soufi
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Robustness of FCS (Fluorescence Correlation Spectroscopy) with Quenchers Present [PDF]
The Journal of Physical Chemistry A, 2019 Inspired by recent publications doubtful of the FCS technique, we scrutinize how irreversible (static) and reversible (dynamic) quenching can influence the interpretation of such data. We consider intermediate cases where the assessment of photophysics (static quenching, blinking-like triplet state relaxation) influence on autocorrelation curves can be
Hima Nagamanasa Kandula +2 more
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Communications Biology, 2023 Fluorescence correlation spectroscopy (FCS) is a single molecule sensitive tool for the quantitative measurement of biomolecular dynamics and interactions.
Jagadish Sankaran, Thorsten Wohland
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Defining the subcellular interface of nanoparticles by live-cell imaging. [PDF]
PLoS ONE, 2013 Understanding of nanoparticle-bio-interactions within living cells requires knowledge about the dynamic behavior of nanomaterials during their cellular uptake, intracellular traffic and mutual reactions with cell organelles. Here, we introduce a protocol
Peter H Hemmerich, Anna H von Mikecz
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Covalent dye attachment influences the dynamics and conformational properties of flexible peptides. [PDF]
PLoS ONE, 2017 Fluorescence spectroscopy techniques like Förster resonance energy transfer (FRET) and fluorescence correlation spectroscopy (FCS) have become important tools for the in vitro and in vivo investigation of conformational dynamics in biomolecules.
Manuel P Luitz +5 more
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Wnt3 Is Lipidated at Conserved Cysteine and Serine Residues in Zebrafish Neural Tissue
Frontiers in Cell and Developmental Biology, 2021 Wnt proteins are a family of hydrophobic cysteine-rich secreted glycoproteins that regulate a gamut of physiological processes involved in embryonic development and tissue homeostasis.
Divya Dhasmana +9 more
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Tracking-FCS: Fluorescence correlation spectroscopy of individual particles
Optics Express, 2005 We exploit recent advances in single-particle tracking to perform fluorescence correlation spectroscopy on individual fluorescent particles, in contrast to traditional methods that build up statistics over a sequence of many measurements. By rapidly scanning the focus of an excitation laser in a circular pattern, demodulating the measured fluorescence,
Andrew, Berglund, Hideo, Mabuchi
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Fluorescein Derivatives as Antibacterial Agents Acting via Membrane Depolarization
Biomolecules, 2020 Appending a lipophylic alkyl chain by ester bond to fluorescein has been previously shown to convert this popular dye into an effective protonophoric uncoupler of oxidative phosphorylation in mitochondria, exhibiting neuro- and nephroprotective effects ...
Pavel A. Nazarov +9 more
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Molecules, 2022 Measurement of the nitric oxide (NO) concentration in living cells in the physiological nanomolar range is crucial in understanding NO biochemical functions, as well as in characterizing the efficiency and kinetics of NO delivery by NO-releasing drugs ...
Roksana Markiewicz +5 more
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