Results 21 to 30 of about 7,002 (182)
Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy: Concepts and Applications
Molecules, 2018 We review the basic concepts and recent applications of two-dimensional fluorescence lifetime correlation spectroscopy (2D FLCS), which is the extension of fluorescence correlation spectroscopy (FCS) to analyze the correlation of fluorescence lifetime in
Takuhiro Otosu, Shoichi Yamaguchi
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Allele-specific endogenous tagging and quantitative analysis of β-catenin in colorectal cancer cells
eLife, 2022 Wnt signaling plays important roles in development, homeostasis, and tumorigenesis. Mutations in β-catenin that activate Wnt signaling have been found in colorectal and hepatocellular carcinomas.
Giulia Ambrosi +5 more
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BioTechniques, 1998 The yield of the double-stranded DNA product (500 bp) of asymmetric PCR with a rhodamine-labeled primer (Rho-primer) was determined in a homogeneous solution using fluorescence correlation spectroscopy (FCS).
Masataka Kinjo
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Single-molecule detection on a portable 3D-printed microscope
Nature Communications, 2019 Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting ...
James W. P. Brown +7 more
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PLoS ONE, 2019 Fluorescence fluctuation spectroscopy (FFS) refers to techniques that analyze fluctuations in the fluorescence emitted by fluorophores diffusing in a small volume and can be used to distinguish between populations of molecules that exhibit differences in
Nathan Scales, Peter S Swain
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Optimizing Photophysical Conditions in Confocal Fluorescence Correlation Spectroscopy (FCS) [PDF]
Biophysical Journal, 2012 FCS is a powerful method to measure diffusional and rotational properties of molecules as well as intramolecular transitions (e.g. triplet transition rates and isomerization) and intermolecular interactions (e.g. protein-protein, DNA-protein, etc.). Since the introduction of the confocal measuring principle by Rigler et al.
Opitz, Norbert H. +2 more
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Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy
Membranes, 2021 Although conventional fluorescence intensity imaging can be used to qualitatively study the drug toxicity of nanodrug carrier systems at the single-cell level, it has limitations for studying nanodrug transport across membranes.
Xinwei Gao +8 more
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Applied Sciences, 2021 Multiphoton laser scanning microscopy (MPM) has opened up an optical window into biological tissues; however, imaging is primarily qualitative. Cell morphology and tissue architectures can be clearly visualized but quantitative analysis of actual ...
Jeemol James +2 more
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Nanowaveguide-illuminated fluorescence correlation spectroscopy for single molecule studies
AIP Advances, 2021 Fluorescence Correlation Spectroscopy (FCS) is a method of investigating concentration fluctuations of fluorescent particles typically in the nM range as a result of its femtoliter-sized sample volume. However, biological processes on cell membranes that
Joseph M. Chandler, Huizhong Xu
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Fluorescence correlation spectroscopy for the characterization of membranes : A short review [PDF]
Songklanakarin Journal of Science and Technology (SJST), 2002 Fluorescence correlation spectroscopy (FCS) is a highly sensitive fluorescence microscopy technique that can be used to probe a wide range of biophysical processes including diffusion, Ligand-receptor binding and molecular aggregation on artificial and ...
Hwang, L.C., Thorsten Wohland
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