Results 51 to 60 of about 7,002 (182)
In this study, the concentration and brightness measured by fluorescence correlation spectroscopy (FCS) in samples containing multiple species with different brightness levels was demonstrated.
Johtaro Yamamoto, Akira Sasaki
doaj +1 more source
ABSTRACT Extracellular vesicles (EVs) are nanoscale membrane‐bound structures that play a pivotal role in intercellular communication by transporting bioactive molecules, including proteins, lipids, nucleic acids, and organelles, between cells. Originating from the endosomal pathway, EVs reflect the physiological and pathological status of their parent
Emine İncilay Torunoğlu +3 more
wiley +1 more source
Computational design and interpretation of single-RNA translation experiments.
Advances in fluorescence microscopy have introduced new assays to quantify live-cell translation dynamics at single-RNA resolution. We introduce a detailed, yet efficient sequence-based stochastic model that generates realistic synthetic data for several
Luis U Aguilera +7 more
doaj +1 more source
Messages do diffuse faster than messengers: reconciling disparate estimates of the morphogen bicoid diffusion coefficient. [PDF]
The gradient of Bicoid (Bcd) is key for the establishment of the anterior-posterior axis in Drosophila embryos. The gradient properties are compatible with the SDD model in which Bcd is synthesized at the anterior pole and then diffuses into the embryo ...
Lorena Sigaut +3 more
doaj +1 more source
Abstract Flow cytometry measures the optical properties of individual cells and has become essential for enumerating and characterizing marine phytoplankton. Yet systematic comparison of measurements across instruments remains limited, hampering data comparison across laboratories and long‐term time series.
Francois Ribalet +10 more
wiley +1 more source
The plasma membrane organization of live cells defines a plethora of cellular processes important for cell functionality. Many membrane structures that define this organization exist at a spatial resolution below the optical diffraction limit ...
Xue Wen Ng +2 more
doaj +1 more source
We characterize a novel fluorescence microscope which combines the high spatial discrimination of a total internal reflection epi-fluorescence (epi-TIRF) microscope with that of stimulated emission depletion (STED) nanoscopy. This combination of high axial confinement and dynamic-active lateral spatial discrimination of the detected fluorescence ...
Leutenegger, Marcel +4 more
openaire +6 more sources
Tuning the Length of Filomicelles Prepared Via Templated Polymerization‐Induced Self‐Assembly
High‐aspect‐ratio polymeric micelles are attractive yet notoriously difficult to control in length. We reveal a counterintuitive and powerful solution: in supramolecularly templated PISA, adding non‐sticker polymer unexpectedly elongates the micelles, while post‐assembly sonication enables precise shortening with reduced dispersity.
Eleonora G. Hochreiner +10 more
wiley +1 more source
Dodecyl ether derivatives 1-3 of p-sulfonatocalix[n]arene were incorporated into dimyristoyl phosphatidylcholine (DMPC) vesicles, and their binding abilities for acetylcholine (ACh) were examined by using steady-state fluorescence/fluorescence anisotropy
Yasuhiro Ooi +2 more
doaj +1 more source
Macromolecular crowding (MMC) in cells is a hot topic in biology; therefore, well-characterized measurement standards for the evaluation of the nano-environment in MMC solutions are necessary.
Johtaro Yamamoto +7 more
doaj +1 more source

