Results 51 to 60 of about 7,002 (182)

Measurement of the Concentration and the Brightness for Samples Containing Multiple Molecules with Different Brightness Using Fluorescence Correlation Spectroscopy

open access: yesApplied Sciences, 2021
In this study, the concentration and brightness measured by fluorescence correlation spectroscopy (FCS) in samples containing multiple species with different brightness levels was demonstrated.
Johtaro Yamamoto, Akira Sasaki
doaj   +1 more source

Extracellular Vesicles‐Based Biological Macromolecule Delivery Systems: Biogenesis, Characterization, and Emerging Clinical Applications

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Extracellular vesicles (EVs) are nanoscale membrane‐bound structures that play a pivotal role in intercellular communication by transporting bioactive molecules, including proteins, lipids, nucleic acids, and organelles, between cells. Originating from the endosomal pathway, EVs reflect the physiological and pathological status of their parent
Emine İncilay Torunoğlu   +3 more
wiley   +1 more source

Computational design and interpretation of single-RNA translation experiments.

open access: yesPLoS Computational Biology, 2019
Advances in fluorescence microscopy have introduced new assays to quantify live-cell translation dynamics at single-RNA resolution. We introduce a detailed, yet efficient sequence-based stochastic model that generates realistic synthetic data for several
Luis U Aguilera   +7 more
doaj   +1 more source

Messages do diffuse faster than messengers: reconciling disparate estimates of the morphogen bicoid diffusion coefficient. [PDF]

open access: yesPLoS Computational Biology, 2014
The gradient of Bicoid (Bcd) is key for the establishment of the anterior-posterior axis in Drosophila embryos. The gradient properties are compatible with the SDD model in which Bcd is synthesized at the anterior pole and then diffuses into the embryo ...
Lorena Sigaut   +3 more
doaj   +1 more source

Flow cytometric phytoplankton concentration and optical properties across instruments and laboratories

open access: yesLimnology and Oceanography: Methods, EarlyView.
Abstract Flow cytometry measures the optical properties of individual cells and has become essential for enumerating and characterizing marine phytoplankton. Yet systematic comparison of measurements across instruments remains limited, hampering data comparison across laboratories and long‐term time series.
Francois Ribalet   +10 more
wiley   +1 more source

Characterization of Lipid and Cell Membrane Organization by the Fluorescence Correlation Spectroscopy Diffusion Law

open access: yesCHIMIA, 2015
The plasma membrane organization of live cells defines a plethora of cellular processes important for cell functionality. Many membrane structures that define this organization exist at a spatial resolution below the optical diffraction limit ...
Xue Wen Ng   +2 more
doaj   +1 more source

Fluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS)

open access: yesOptics Express, 2012
We characterize a novel fluorescence microscope which combines the high spatial discrimination of a total internal reflection epi-fluorescence (epi-TIRF) microscope with that of stimulated emission depletion (STED) nanoscopy. This combination of high axial confinement and dynamic-active lateral spatial discrimination of the detected fluorescence ...
Leutenegger, Marcel   +4 more
openaire   +6 more sources

Tuning the Length of Filomicelles Prepared Via Templated Polymerization‐Induced Self‐Assembly

open access: yesSmall, EarlyView.
High‐aspect‐ratio polymeric micelles are attractive yet notoriously difficult to control in length. We reveal a counterintuitive and powerful solution: in supramolecularly templated PISA, adding non‐sticker polymer unexpectedly elongates the micelles, while post‐assembly sonication enables precise shortening with reduced dispersity.
Eleonora G. Hochreiner   +10 more
wiley   +1 more source

Interfacial Recognition of Acetylcholine by an Amphiphilic p-Sulfonatocalix[8]arene Derivative Incorporated into Dimyristoyl Phosphatidylcholine Vesicles

open access: yesSensors, 2008
Dodecyl ether derivatives 1-3 of p-sulfonatocalix[n]arene were incorporated into dimyristoyl phosphatidylcholine (DMPC) vesicles, and their binding abilities for acetylcholine (ACh) were examined by using steady-state fluorescence/fluorescence anisotropy
Yasuhiro Ooi   +2 more
doaj   +1 more source

Quantitative evaluation of macromolecular crowding environment based on translational and rotational diffusion using polarization dependent fluorescence correlation spectroscopy

open access: yesScientific Reports, 2021
Macromolecular crowding (MMC) in cells is a hot topic in biology; therefore, well-characterized measurement standards for the evaluation of the nano-environment in MMC solutions are necessary.
Johtaro Yamamoto   +7 more
doaj   +1 more source

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