Results 61 to 70 of about 7,002 (182)

Quantitative Imaging of Human Hemoglobin Binding to Staphylococcus aureus With Single‐Molecule Resolution

Small, EarlyView.
Single‐molecule localization microscopy is exploited to quantify the number of hemoglobin copies bound to specific receptors on S. aureus wall. This quantification supports the design of a photoactive derivative of hemoglobin able to photo‐inactivate S.
Pietro Delcanale, Andrea Mussini, Òscar Gulías, Montserrat Agut, Melissa Berni, Stefano Pongolini, Stefano Bruno, Alessandro Casnati, Santi Nonell, Cristiano Viappiani, Stefania Abbruzzetti   +10 more
wiley   +1 more source

Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy. [PDF]

PLoS ONE, 2012
Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous cells and in cells synchronized at ...
Ronan Broderick, Sivaramakrishnan Ramadurai, Katalin Tóth, Denisio M Togashi, Alan G Ryder, Jörg Langowski, Heinz Peter Nasheuer   +6 more
doaj   +1 more source

Functional Repurposing of Magnetic Nanoparticle‐Assisted Hyperthermia for Immune Cell Labelling and Tracking

Small Methods, EarlyView.
We propose mild‐MHT protocol for the quick internalization of magnetic nanoparticles (MNPs) by immune cells. The heat induced by MNPs under alternating magnetic field stimulate MNPs diffusion and cell membrane invagination, accelerating the cellular internalization of MNPs. ABSTRACT Immune cell therapies show promise in hematologic malignancies and are
Giulia E. P. Nucci, Chiara Vitale, Alessandro di Girolamo, Niccolò Silvestri, Stefano Persano, Lorenci Gjurgjaj, Cristina Bottino, Roberta Castriconi, Teresa Pellegrino   +8 more
wiley   +1 more source

Quantifying Protein Binding to Lipid Vesicles Using Fluorescence Correlation Spectroscopy (FCS) [PDF]

Biophysical Journal, 2011
Quantitative evaluation of protein binding to liposomes through the determination of their partition coefficient (Kp) is an important step in any lipid-protein interaction study because it allows the calculation of protein interfacial coverage of the lipid vesicles, often the critical parameter controlling the protein membrane binding mode (peripheral ...
Melo, Ana M., Coutinho, Ana, Prieto, Manuel   +2 more
openaire   +1 more source

Fractionation‐Free Protein Corona Quantification Through Synchrotron‐Based Small‐Angle X‐ray Scattering

Small Methods, EarlyView.
A fractionation‐free SAXS approach quantifies protein corona formation on silica nanoparticles under native conditions. By deconvoluting scattering from free and bound proteins, adsorbed mass is directly determined in simple and complex media. The method resolves adsorption regimes and thermodynamics, establishing non‐invasive, in situ characterization
Juliana T. T. Carvalho, Caroline E. P. Silva, Lindomar J. C. Albuquerque, Antônio A. Malfatti‐Gasperini, Liming Wang, Nathan P. Cowieson, Mateus B. Cardoso   +6 more
wiley   +1 more source

Unravelling drug delivery using in vitro Fluorescence Correlation Spectroscopy (FCS)

Journal of Controlled Release
Therapeutic cargo, such as small molecule drugs, proteins and nucleic acids, can only fulfill their function in an effective way if they reach their biological target site intact and at sufficiently high concentrations. To facilitate and steer this transport process after administration and mitigate limited solubility and/or stability, the therapeutic ...
van den Hoven, Lennart, Goddaer, Sarah, Mirzahossein, Elham, Vermonden, Tina, Koynov, Kaloian, Remaut, Katrien, van Ravensteijn, Bas G.P.   +6 more
openaire   +5 more sources

Brain metastasis‐associated fibroblasts shape the tumour microenvironment to enhance cancer cell invasion

Brain Pathology, EarlyView.
Brain metastasis‐associated fibroblasts are active stromal components of the metastatic niche characterised by extracellular matrix remodelling and production of pro‐migratory mediators. They promote monocyte and cancer cell migration and enhance cancer cell invasion while having no growth‐promoting effect on cancer cells.
Barbora Výmolová, Lucie Pfeiferová, Tadeáš Karel Smetana, Petr Výmola, Nikola Ternerová, Michal Zubaľ, Michal Kolář, Jana Šáchová, Soňa Gyönyörová, Eva Balážiová, Magdalena Houdová Megová, Jakub Červenka, Helena Kupcová Skalníková, Michal Španko, Karel Smetana Jr., Lukáš Lacina, David Netuka, Robert Tomáš, Petr Bušek, Aleksi Šedo   +19 more
wiley   +1 more source

Pitching Single-Focus Confocal Data Analysis One Photon at a Time with Bayesian Nonparametrics

Physical Review X, 2020
Fluorescence time traces are used to report on dynamical properties of molecules. The basic unit of information in these traces is the arrival time of individual photons, which carry instantaneous information from the molecule, from which they are ...
Meysam Tavakoli, Sina Jazani, Ioannis Sgouralis, Omer M. Shafraz, Sanjeevi Sivasankar, Bryan Donaphon, Marcia Levitus, Steve Pressé   +7 more
doaj   +1 more source

The histone deacetylase inhibitor, suberoylanilide hydroxamic acid, restores blood–brain barrier integrity in a human stem cell‐based model of ischaemic stroke

British Journal of Pharmacology, EarlyView.
Ischaemic stroke is characterised by acute cerebrovascular occlusion and blood–brain barrier (BBB) breakdown. Our results indicated that the histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) ameliorated the loss of BBB integrity, changed the morphology of brain endothelial cells, increased the level of basement membrane and ...
Anikó Szecskó, Koppány Párdi, Zuhao Cui, Gergő Porkoláb, Zsófia Hoyk, Csilla Kovács, Janet F. Adegbite, Virág Fodor, Nárcisz M. Cser, László Dér, Krisztina Nagy, Csilla Sajben, Roland Tengölics, Mária A. Deli, Szilvia Veszelka   +14 more
wiley   +1 more source

Protocol for In Vivo Two-Photon FCS to Measure Nanocarrier Number and Flow Velocity in Mouse Cerebral Microvasculature

Bio-Protocol
Real-time measurement of blood flow and nanocarrier transport in the cerebral microvasculature is crucial for understanding neurovascular physiology and nanocarrier-based drug delivery.
Sagar Pande, Xiaojin Wang, Xu Fu, Harini Prathapasinghe, Christopher Richards   +4 more
doaj   +1 more source