Results 81 to 90 of about 7,002 (182)

Perspective: fluorescence lifetime imaging and single-molecule spectroscopy for studying biological condensates

Methods in Microscopy
Biological condensates, often formed via liquid-liquid phase separation (LLPS), are membraneless compartments organizing biochemical reactions. Recent advances have shifted the focus from identifying condensates to elucidating their dynamic biological ...
Loidolt-Krüger Maria
doaj   +1 more source

Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy

Biochemistry and Biophysics Reports, 2018
Normal function and abnormal aggregation of transactivation response (TAR) DNA/RNA-binding protein 43 kDa (TDP-43) are directly associated with the lethal genetic diseases: cystic fibrosis, amyotrophic lateral sclerosis (ALS), and frontotemporal lobar ...
Akira Kitamura, Ai Shibasaki, Kayo Takeda, Ryoji Suno, Masataka Kinjo   +4 more
doaj   +1 more source

Redox‐Dependent Chaperoning of GBF1 Condensates Regulates Seed Germination in Arabidopsis

Advanced Science, Volume 13, Issue 47, 24 August 2026.
In dormant seeds (low ROS), GBF1 forms liquid condensates to repress the germination gene CathB3, and the chaperone GIP1 maintains condensate liquidity and repressive activity. Upon imbibition (high ROS), ROS oxidize GIP1 during germination, impairing its chaperone function.
Yunying Wang, Xiaofeng Fang
wiley   +1 more source

The Host Cell Factor Phosphatase‐2A Subunit PR130 Restricts Replication of Herpes Simplex Virus Type‐1

Advanced Science, Volume 13, Issue 44, 7 August 2026.
Molecular, genetic, virological, and biochemical analysis in combination with global proteome and phosphoproteome profiling and functional assays were applied to study the role of PR130 in the context of HSV‐1 replication. The observations reveal that host‐intrinsic mechanisms regulate HSV‐1 replication and highlight PR130 as a susceptibility factor of
Johannes Jungwirth, Christoph F. Jacob, Alexandra Nguyen, Mandy Beyer, Andreas O. Mieland, Mario Dejung, Jia‐Xuan Chen, Walburgis Brenner, Christina Ehrhardt, Andreas Henke, Oliver H. Krämer   +10 more
wiley   +1 more source

Dipole‐Manipulated Built‐In Electric Field Enables Ultrafast Charge Separation in Perylene Diimide Polymers for Photoelectrochemical Water Splitting

Advanced Science, Volume 13, Issue 43, 3 August 2026.
Molecular dipole engineering in dendritic perylene diimide polymers through nitrogen‐rich heterocyclic linkers amplifies the built‐in electric field, enabling sub‐picosecond charge separation and efficient multi‐electron accumulation. This structure–dynamics–performance relationship establishes a versatile design strategy for high‐performance organic ...
Ying‐Xin Qiao, Zicong Situ, Yi‐Jing Chen, Shuo‐Xiang Liu, Jing‐Lan Zhang, Xingqing Li, Luo‐Han Xie, Si‐Hang Xie, Qing‐Xiao Tong, Andong Xia, Zhuoran Kuang, Jing‐Xin Jian   +11 more
wiley   +1 more source

STED-FCS in subdiffraction limit volumes reveals altered diffusion in live cell applications

Methods in Microscopy
Fluorescence correlation spectroscopy (FCS) is a widely established light microscopy technique for investigating physiological parameters such as diffusion states, particle numbers, and viscosity in biological samples.
Zobiak Bernd, Andres-Alonso Maria, Wang Yuanyuan, Mohammadi Behnam, Gorzelanny Christian, Altmeppen Hermann Clemens, Yin Shuting, Failla Antonio Virgilio   +7 more
doaj   +1 more source

Building a Fluorescence Correlation Spectroscopy (FCS) instrument followed bytesting the boundaries of FRET-FCS

, 2021
Understanding biological systems oftentimes requires mapping the behaviors of biomolecules on small scales such as single proteins or nucleic acids. Fluorescence Correlation Spectroscopy (FCS) is a group of particularly noninvasive and sensitive fluorescence-­based techniques that can be used for this purpose. In this project an FCS-­apparatus has been
Weinberg-Krakowski, Isabell, Stenseth, Evelina   +1 more
openaire   +1 more source

Imaging‐Guided Live Single‐Cell Lipid Profiling of Leader and Follower Cells During Collective Migration of Triple‐Negative Breast Cancer Cells

Advanced Science, Volume 13, Issue 47, 24 August 2026.
Integrated single‐cell mass spectrometry reveals distinct lipidomic signatures that differentiate leader and follower phenotypes in migratory breast cancer cells. Phenotype‐specific alterations across various lipid classes, including fatty acids and phosphatidylcholines, underpin functional heterogeneity.
Xiaoyue Huang, Judit González Riera, Mai Nguyen, Jeremy Broekhuis, Sylvia E. Le Dévédec, Ahmed Ali, Thomas Hankemeier   +6 more
wiley   +1 more source

Current Challenges of Transcription Compartmentalization Research

Advanced Science, Volume 13, Issue 46, 17 August 2026.
Transcription factors, coactivators, and RNA polymerase II assemble into transcription compartments ranging from small, defined complexes to liquid‐like condensates. This review unifies these seemingly competing descriptions along a single continuum and asks what these compartments have been shown to do, and what they have not, revealing that the most ...
Thomas Quail, Sina Wittmann
wiley   +1 more source

Age‐associated immune dysregulation links to disease severity in macrolide‐resistant Mycoplasma pneumoniae pneumonia: Insights from a comparative juvenile–adult mouse model

Animal Models and Experimental Medicine, Volume 9, Issue 8, Page 1489-1506, August 2026.
Macrolide‐resistant Mycoplasma pneumoniae (MRMP) infected both juvenile and adult mice, with results compared to normal juvenile mice. The findings revealed comparable bacterial loads between juvenile and adult mice, yet juvenile mice exhibited more severe lung damage and elevated levels of inflammatory cytokines.
Xuejun Li, Yudong Wang, Liyong Liu, Mengmeng Gong, Jinlong Wang, Yuqing Cao, Zhixin Sun, Fanliang Meng, Qiuyan Wang, Hongji Wu, Fei Zhao, Yan Ma, Yibai Xiong   +12 more
wiley   +1 more source