Results 41 to 50 of about 984,356 (291)

Two-photon-excited fluorescence (TPEF) and fluorescence lifetime imaging (FLIM) with sub-nanosecond pulses and a high analog bandwidth signal detection

open access: yes, 2017
Two-photon excited fluorescence (TPEF) microscopy and fluorescence lifetime imaging (FLIM) are powerful imaging techniques in bio-molecular science. The need for elaborate light sources for TPEF and speed limitations for FLIM, however, hinder an even ...
Eibl, Matthias   +4 more
core   +1 more source

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

open access: yes, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe   +295 more
core   +2 more sources

Fiber-optic fluorescence imaging [PDF]

open access: yesNature Methods, 2005
Optical fibers guide light between separate locations and enable new types of fluorescence imaging. Fiber-optic fluorescence imaging systems include portable handheld microscopes, flexible endoscopes well suited for imaging within hollow tissue cavities and microendoscopes that allow minimally invasive high-resolution imaging deep within tissue.
Benjamin A, Flusberg   +5 more
openaire   +2 more sources

Single- and multi-photon excited fluorescence from serotonin complexed with B-cyclodextrin [PDF]

open access: yes, 2006
The fluorescence of serotonin on binding with B-cyclodextrin has been studied using both steady-state and time-resolved methods. Steady state fluorescence intensity of serotonin at 340 nm showed ~ 30% increase in intensity on binding with Ka ~ 60 dm3 mol
Anthony W. Parker   +27 more
core   +1 more source

Organoids in pediatric cancer research

open access: yesFEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Application of near-infrared fluorescence imaging with indocyanine green in mediastinal surgeryCentral MessagePerspective

open access: yesJTCVS Techniques, 2023
Intraoperative near-infrared (NIR) fluorescence imaging has emerged in recent years and is now used in many surgical procedures. Intraoperative fluorescence imaging can guide surgeons in identifying and localizing specific structures and boundaries ...
Yilan Cao   +2 more
doaj   +1 more source

An ytterbium quantum gas microscope with narrow-line laser cooling [PDF]

open access: yes, 2015
We demonstrate site-resolved imaging of individual bosonic $^{174}\mathrm{Yb}$ atoms in a Hubbard-regime two-dimensional optical lattice with a short lattice constant of 266 nm.
Kato, Kohei   +4 more
core   +2 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

Trends in Single-Molecule Total Internal Reflection Fluorescence Imaging and Their Biological Applications with Lab-on-a-Chip Technology

open access: yesSensors, 2023
Single-molecule imaging technologies, especially those based on fluorescence, have been developed to probe both the equilibrium and dynamic properties of biomolecules at the single-molecular and quantitative levels. In this review, we provide an overview
Louis Colson   +6 more
doaj   +1 more source

Moxifloxacin: Clinically compatible contrast agent for multiphoton imaging [PDF]

open access: yes
Multiphoton microscopy (MPM) is a nonlinear fluorescence microscopic technique widely used for cellular imaging of thick tissues and live animals in biological studies.
AHN, G ONE   +21 more
core   +1 more source

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