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Raman-Fluorescence in Situ Hybridization
2014As with many other molecular microbiology methods, the family of stable isotope probing (SIP) techniques based on the analysis of isotope- labeled nucleic acids, phospholipid fatty acids (PLFAs), or proteins are frequently performed at the level of the community.
Read, Daniel, Whiteley, Andrew
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Fluorescence in situ hybridization
Cancer Genetics and Cytogenetics, 2001There is a rumor that goes like this: After a particularly successful day at an international genetics conference in the late 1960s in Scotland, delegates rewarded themselves with the customary pint of beer at their favorite watering hole, the public house.
P.S Amare +5 more
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Advances in fluorescence in situ hybridization
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1998The techniques of in situ hybridization (ISH) are widely applied for analyzing the genetic make-up and RNA expression patterns of individual cells. This review focusses on a number of advances made over the last 5 years in the fluorescence ISH (FISH) field, i.e., Fiber-FISH, Multi-colour chromosome painting, Comparative Genomic Hybridization, Tyramide ...
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Fluorescence In Situ Hybridization
1999Within the past decade, the cytogenetics laboratory has witnessed a major advance in diagnostic and prognostic capability with the advent of molecular cytogenetics. The product of a combination of cytogenetics and molecular biology, the technique of fluorescence in situ hybridization (FISH), has increased the resolution and application of traditional ...
Gesa Schwanitz, Regine Schubert
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Fluorescence In Situ Hybridization in Oat
2017This chapter describes methods to detect gene loci or gene transcripts by fluorescence labeling. Fluorescence in situ hybridization (FISH) can be used to identify the positions of genes or BACs or the distribution of repetitive sequences on metaphase chromosomes as well as the identification of alien chromosomes.
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Fluorescence In situ Hybridization
1996During the last decade, pathology has progressed remarkably with the incorporation of molecular techniques into the arena of diagnostics. Fluorescence in situ hybridization (FISH) is a molecular histopathological technique that can be readily used to identify DNA or RNA abnormalities at the cellular level with the use of an epifluorescence microscope ...
Sunny Luke +3 more
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Fluorescent In Situ Hybridization
2001Fluorescence in situ hybridization (FISH) arose from a marriage of classical DNA hybridization in solution to modern molecular biologic techniques, most notably the use of restriction endonucleases and, later, the polymerase chain reaction (PCR). The former has made identification of both genes and relevant interspersed sequences possible, whereas ...
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Fluorescence in situ hybridization
2007Princip hibridizacijskih tehnika in situ počiva na komplementarnom vezanju obilježene sonde sa specifičnim odsječkom DNA na citogenetskom preparatu, što omogućava izravno utvrđivanje i lokalizaciju određenih sljedova DNA na kromosomu. Stoga ove metode imaju mnoge primjene u istraživanju i dijagnostici (npr.
Mravinac, Brankica +1 more
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Standard Fluorescence in situ Hybridization Procedures [PDF]
Fluorescence in situ hybridization (FISH) allows nucleic acid sequences to be visualized inside fi xed cells or chromosomes. The method, based on the principle that nucleic acids can denature and renature, allows the detection of a known nucleotide sequence (probe), in a cell/chromosome, thanks to its ability to hybridize the complementary nucleic-acid
Bonillo C +5 more
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Fluorescence in situ Hybridization
2017Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique used to physically map DNA sequences on chromosomes. In FISH, DNA probes are most often hybridized to target DNA in metaphase chromosomes spread on slides (in situ). FISH probes are variable in size.
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