Results 301 to 310 of about 216,526 (358)

Fluorescence In Situ Hybridization

2021
Fluorescence in situ hybridization (FISH) is a method of using fluorescent probes to detect specific nucleic acid sequences within cells and their location. It can be used to detect a variety of cytogenetic variations, including chromosomal deletions, amplification, and translocation.
Min Hu, Weimin Wu
openaire   +3 more sources

Fluorescence In Situ Hybridization

2003
In situ hybridization describes the annealing of a labeled nucleic acid to complementary nucleic acid sequences in a fixed target (e.g., chromosomes, free nuclei, nuclei in tissue sections, and DNA) followed by visualisation of the location of the probe.
Rachel L. Ostroff, Barbara A. Tate
openaire   +6 more sources

Fluorescent In Situ Hybridization

2003
Single-stranded DNA will recognize a complementary strand with high specificity under suitably controlled conditions. In situ hybridization (ISH) exploits this phenomenon by hybridizing an appropriately labeled singlestranded DNA “probe” to target sequences in situ in either dissociated cell preparations or tissue sections.
Elaine K. Green, Sara A. Dyer
openaire   +3 more sources

Fluorescence In Situ Hybridization

1999
Within the past decade, the cytogenetics laboratory has witnessed a major advance in diagnostic and prognostic capability with the advent of molecular cytogenetics. The product of a combination of cytogenetics and molecular biology, the technique of fluorescence in situ hybridization (FISH), has increased the resolution and application of traditional ...
Gesa Schwanitz, Regine Schubert
openaire   +4 more sources

Fluorescence in situ hybridization

Blood Reviews, 1993
Spectacular advances in the use of fluorescence in situ hybridization (FISH) for the visualisation of specific DNA sequences in metaphase chromosomes and interphase cells have been made over the last few years making the technique a useful tool in clinical research.
openaire   +2 more sources

Fluorescence In Situ Hybridization

Clinics in Laboratory Medicine, 2011
This chapter presents past and present FISH techniques and specific applications of FISH. Although array technology has revolutionized cytogenetics, FISH remains indispensible. While array technology provides a high resolution screen of the entire genome for gains and losses, it does not allow for visualization of the genomic structure of gains.
openaire   +2 more sources

Fluorescence in situ hybridization: 1998

Luminescence, 1999
The journal provides comprehensive literature searches on all aspects of luminescence which will be published in most issues. These lists have been produced regularly since 1986 and in excess of 5500 references have been cited—some by year and some by specialized topic.
Larry J. Kricka, P. E. Stanley
openaire   +3 more sources

An Introduction to Fluorescence in situ Hybridization in Microorganisms

2021
Fluorescence in situ hybridization (FISH) is a molecular biology technique that enables the localization, quantification, and identification of microorganisms in a sample. This technique has found applications in several areas, most notably the environmental, for quantification and diversity assessment of microorganisms and, the clinical, for the rapid
Almeida, Carina, Azevedo, Nuno F.
openaire   +4 more sources

Fluorescent In Situ Hybridization

2008
In situ hybridization (ISH) technique was introduced by Gall and Pardue1 in 1969. At that time the technique was limited by the use of radioactively labelled probes that were subsequently visualized by autoradiography. The development of interphase cytogenetics in the 1980s and fluorescent labels in 19862 has seen the technology applied in a number of ...
Fiona Campbell, John M. S. Bartlett
openaire   +2 more sources

Probe Labeling and Fluorescence In Situ Hybridization [PDF]

Current Protocols in Cytometry, 1997
AbstractThis unit describes in detail basic protocols for probe labeling, denaturing of in situ target DNA, in situ hybridization, and post‐hybridization washes. Support protocols for probe labeling cover probe purification and quality control.
A.K. Raap, J. Wiegant
openaire   +2 more sources