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Fluorescence In Situ Hybridization
2005Dr. Seuss’s eloquent “One FISH, two FISH, red FISH, blue FISH” (1) could have been describing one of the most significant advancements in clinical cytogenetics, fluorescence in situ hybridization (FISH). The process, as described by Pinkel et al. in 1988 (2), involved fluorescent detection of probe DNA hybridized to chromosomal target sequences.
Stuart Schwartz +2 more
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Fluorescence in situ hybridization: Uses and limitations
Seminars in Hematology, 2000The development of molecular hybridization techniques such as fluorescence in situ hybridization (FISH) has had a major Impact on efforts to detect and characterize the genetic changes that give rise to human tumors. With probes designed to Identify specific chromosomes and chromosomal regions, FISH is used routinely by cytogenetics and pathology ...
Gozzetti, A, Le Beau, M M
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Fluorescent In Situ Hybridization (FISH)
2021In situ hybridization (ISH) is a technique that uses nucleic acid probes to allow for visualization of nucleic acid sequences in cellular/tissue preparations. Both enzymatic and fluorescent ISH studies can be used as adjunct studies, and in some cases are vital to making specific diagnoses. This chapter will focus on fluorescent in situ hybridization.
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Fluorescence In situ Hybridization
1996During the last decade, pathology has progressed remarkably with the incorporation of molecular techniques into the arena of diagnostics. Fluorescence in situ hybridization (FISH) is a molecular histopathological technique that can be readily used to identify DNA or RNA abnormalities at the cellular level with the use of an epifluorescence microscope ...
Victoria Belogolovkin +6 more
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Fluorescent In Situ Hybridization
2001Fluorescence in situ hybridization (FISH) arose from a marriage of classical DNA hybridization in solution to modern molecular biologic techniques, most notably the use of restriction endonucleases and, later, the polymerase chain reaction (PCR). The former has made identification of both genes and relevant interspersed sequences possible, whereas ...
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Raman-Fluorescence in Situ Hybridization
2014As with many other molecular microbiology methods, the family of stable isotope probing (SIP) techniques based on the analysis of isotope- labeled nucleic acids, phospholipid fatty acids (PLFAs), or proteins are frequently performed at the level of the community.
Read, Daniel, Whiteley, Andrew
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Fluorescence In Situ Hybridization (
AbstractFluorescence in situ Hybridization (FISH) involves the preparation of two main components: the DNA probe and the target DNA to which the probe will be hybridized. The DNA probe typically comes from cloned sources such as plasmids, cosmids, PACs, YACs, or BACs; where the insert may contain a specific gene or originate from a specific chromosomal
Jeremy A. Squire, Jane Bayani
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Blaming the Right Fluorescent in Situ Hybridization
Journal of Clinical Oncology, 2015TO THE EDITOR: Bochtler et al have observed that patients with light chain (AL) amyloidosis with t(11;14) fare worse with bortezomib-based therapy. This observation is difficult to reconcile given both the fact that a majority of patients with AL have t(11;14), and that bortezomib has been observed to be a highly effective therapy in patients with AL ...
Angela Dispenzieri, Rahma Warsame
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Advances in fluorescence in situ hybridization
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1998The techniques of in situ hybridization (ISH) are widely applied for analyzing the genetic make-up and RNA expression patterns of individual cells. This review focusses on a number of advances made over the last 5 years in the fluorescence ISH (FISH) field, i.e., Fiber-FISH, Multi-colour chromosome painting, Comparative Genomic Hybridization, Tyramide ...
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Fluorescence in situ hybridization: A brief review
Environmental and Molecular Mutagenesis, 1996Fluorescence in situ hybridization (FISH) is used for many purposes, including analysis of chromosomal damage, gene mapping, clinical diagnostics, molecular toxicology and cross-species chromosome homology. FISH allows an investigator to identify the presence and location of a region of cellular DNA or RNA within morphologically preserved chromosome ...
Roy R. Swiger, James D. Tucker
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