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Fluorescence in situ Hybridization in Cytopathology
2020The application of ancillary studies to small biopsies and cytopathology specimens has been increasing for definitive diagnoses and theranostic information. Fluorescence in situ hybridization (FISH) is one of the ancillary techniques being applied to these small specimens, and has been utilized with great success for years, particularly in the ...
Sanja Dacic, Sara E. Monaco
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Fluorescence In Situ Hybridization and Comparative Genomic Hybridization
2006This chapter discusses the complementary methodologies of fluorescence in situ hybridization and comparative genomic hybridization. Fluorescence in situ hybridization uses fluorescently labeled DNA probes (whole chromosomes, centromere, or locus-specific sequences) to visualize complementary DNA sequences in the target DNA (metaphase chromosomes or ...
Patricia Gorman, Rebecca Roylance
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Fluorescence In Situ Hybridization
2001There is a rumor that goes like this: After a particularly successful day at an international genetics conference in the late 1960s in Scotland, delegates rewarded themselves with the customary pint of beer at their favorite watering hole, the public house.
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Fluorescence In Situ Hybridization and In Situ PCR
2011The application of fluorescence in situ hybridization to chromosomes allows each member of the karyotype to be distinguished from all others. This technique can be used to detect changes in chromosomes. Individual genes and transgenes can be placed to chromosome as well.
James A. Birchler, Tatiana V. Danilova
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Fluorescence in situ hybridization in diagnostic cytology
Human Pathology, 2007Fluorescence in situ hybridization (FISH) is a technique that uses fluorescently labeled DNA probes to detect chromosomal alterations in cells. FISH can detect various types of cytogenetic alterations including aneusomy (ie, abnormalities of chromosome copy number), duplication, amplification, deletion, and translocation.
Benjamin R. Kipp, Kevin C. Halling
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fluorescence in situ hybridization
2017Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique used to physically map DNA sequences on chromosomes. In FISH, DNA probes are most often hybridized to target DNA in metaphase chromosomes spread on slides (in situ). FISH probes are variable in size.
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Clinical applications of fluorescence in situ hybridization
Genetic Analysis: Biomolecular Engineering, 1991We review here the application of fluorescence in situ hybridization with chromosome-specific probes to chromosome classification and to detection of changes in chromosome number or structure associated with genetic disease. Information is presented on probe types that are available for disease detection.
Daniel Pinkel+4 more
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Fluorescence In Situ Hybridization
Fluorescence in situ hybridization (FISH) is a macromolecule recognition technology based on the complementary properties of DNA or DNA/RNA double strands. Unlike most other techniques used to study chromosomes, FISH does not require actively dividing cells.openaire +1 more source
Fluorescence In Situ Hybridization in Oat
2017This chapter describes methods to detect gene loci or gene transcripts by fluorescence labeling. Fluorescence in situ hybridization (FISH) can be used to identify the positions of genes or BACs or the distribution of repetitive sequences on metaphase chromosomes as well as the identification of alien chromosomes.
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Quantitative Fluorescence In Situ Hybridization (QFISH)
2016Fluorescence in situ hybridization (FISH) has a wide spectrum of applications in current molecular cytogenetic and cancer research. This is a unique technique that can be used for chromosomal DNA analysis in all cell types, at all stages of the cell cycle, and at molecular resolution.
Ivan Y. Iourov, Ivan Y. Iourov
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