Results 31 to 40 of about 283,742 (346)

Fluorescence In Situ Hybridization Method for Co-Localization of mRNA and GFP

open access: yesBioTechniques, 2001
Co-localization studies using green fluorescent protein (GFP) and fluorescence immunohistochemistry have become commonplace. However, co-localization studies using GFP and mRNA in situ hybridization are rare, in large part because typical in situ ...
Anthony A. Oliva, John W. Swann
doaj   +1 more source

Double-staining chromogenic in situ hybridization as a useful alternative to split-signal fluorescence in situ hybridization in lymphoma diagnostics

open access: yesHaematologica, 2010
Background Malignant lymphomas are classified based on morphology, immunophenotype, genetics and clinical features. The pathological diagnosis is generally considered difficult and prone to mistakes.
Anke van Rijk   +10 more
doaj   +1 more source

FISH molecular testing in cytological preparations from solid tumors [PDF]

open access: yes, 2014
Many of the exciting new developments in solid tumor molecular cytogenetics impact classical and molecular pathology. Fluorescence in situ hybridization to identify specific DNA target sequences in nuclei of non-dividing cells in solid neoplasms has ...
CARIA, PAOLA, VANNI, ROBERTA
core   +1 more source

Specific metaphase and interphase detection of the breakpoint region in 8q24 of burkitt lymphoma cells by triple-color fluorescence in situ hybridization [PDF]

open access: yes, 1992
Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line Jl was used for the specific delineation of ...
Anastasi   +33 more
core   +1 more source

Infantile nodular fasciitis of the hand: A case report and literature review

open access: yesHuman Pathology: Case Reports, 2016
Pediatric nodular fasciitis is uncommon and has a preference for the head and neck region. Occurrence in other anatomic locations is uncommon. We describe here a case of nodular fasciitis that arose in the hand of a newborn infant who presented with a ...
I Weng Lao   +3 more
doaj   +1 more source

The Roles of the Fluorescent In Situ Hybridization (FISH) and Comparative Genomic Hybridization (CGH) Techniques in the Detection of the Breast Cancer

open access: yesBiology, Medicine & Natural Product Chemistry, 2022
This paper aimed to understand and compare the two popular cytogenetic techniques of fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) in detecting breast cancer chromosomal abnormality.
Harem Othman Smail
doaj   +1 more source

Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]

open access: yes, 1992
Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Brooks-Wilson   +15 more
core   +1 more source

HER 2 status in invasive breast cancer: Immunohistochemistry, fluorescence in-situ hybridization and chromogenic in-situ hybridization

open access: yesIndian Journal of Pathology and Microbiology, 2012
Introduction : HER2/neu gene status in breast cancers can be evaluated by targeting protein and gene - immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH).
Hemlata S Shirsat   +5 more
doaj   +1 more source

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]

open access: yes, 1992
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke   +33 more
core   +1 more source

A pipeline for targeted metagenomics of environmental bacteria. [PDF]

open access: yes, 2020
BackgroundMetagenomics and single cell genomics provide a window into the genetic repertoire of yet uncultivated microorganisms, but both methods are usually taxonomically untargeted.
Bowers, Robert M   +7 more
core   +2 more sources

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