Results 31 to 40 of about 114,945,524 (259)

A Technical Note on Quantum Dots for Multi-Color Fluorescence in situ Hybridization [PDF]

, 2009
Quantum dots (Qdots) are semiconductor nanocrystals, which are photo-stable, show bright fluorescence with narrow, symmetric emission spectra and are available in multiple resolvable colors.
Müller, Stefan, Cremer, T., Neusser, M., Grasser, F., Cremer, M.   +4 more
core   +1 more source

A strategy for the characterization of minute chromosome rearrangements using multiple color fluorescence in situ hybridization with chromosome-specific DNA libraries and YAC clones [PDF]

, 1993
The identification of marker chromosomes in clinical and tumor cytogenetics by chromosome banding analysis can create problems. In this study, we present a strategy to define minute chromosomal rearrangements by multicolor fluorescence in situ ...
Schindler, Detlev, Speicher, Michael R., Riethman, Harold C., Cremer, Thomas, Donis-Keller, Helen, Popp, Susanne, Jauch, Anna, Lengauer, Christoph   +7 more
core   +1 more source

Multicolor chromosome bar codes [PDF]

, 2006
Chromosome bar codes are multicolor banding patterns produced by fluorescence in situ hybridization (FISH) with differentially labeled and pooled sub-regional DNA probes.
Müller, Stefan, Wienberg, Johannes
core   +1 more source

Multiplexed Single-Cell in situ RNA Profiling

Frontiers in Molecular Biosciences, 2021
The ability to quantify a large number of varied transcripts in single cells in their native spatial context is crucial to accelerate our understanding of health and disease.
Yu-Sheng Wang, Jia Guo
doaj   +1 more source

Comparative chromosome band mapping in primates byin situ suppression hybridization of band specific DNA microlibraries [PDF]

, 1991
A DNA-library established from microdissected bands 8q23 to 8q24.1 of normal human chromosomes 8 (Lüdecke et al., 1989) was used as a probe for chromosomal in situ suppression (CISS-) hybridization to metaphase chromosomes of man and primates including ...
Cremer, Thomas, Wienberg, Johannes, Horsthemke, B., Lüdecke, H. J., Lengauer, Christoph, C. Lengauer, T. Cremer, J. Wienberg, B. Horsthemke, H. J. Lüdecke   +9 more
core   +1 more source

Regulation of the lncRNA NEAT1 by p53‐ΔNp63 crosstalk modulates the DNA damage response and therapeutic efficacy in HNSCC

Molecular Oncology, EarlyView.
In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico, Veronica La Banca, Silvia D'Amico, Stefano Scalera, Sara Nicolai, Angelo Peschiaroli   +5 more
wiley   +1 more source

Biological implications of deletion p53 by fluorescence in situ hybridization in multiple myeloma

Biomedical and Biotechnology Research Journal, 2022
Background: Multiple myeloma (MM) is a clonal plasma cell disorder characterized by heterogeneous complex genetic abnormalities. Due to the low proliferative index of plasma cells, conventional cytogenetic (CC) analysis is hampered in MM.
Vijith Vittal Shetty, Meenakshi Arumugam, Reshma Arun Shetty, Akanksha Ashok Kalal, Nagaraj Venkatesh Kulkarni, Deyyenthody Prashanth Shetty   +5 more
doaj   +1 more source

The Price of Precision: A Critical Review of Molecular Diagnostics in Glioma, From Guidelines to Global Disparities

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Gliomas have undergone a profound redefinition over the past decade, transitioning from morphology‐based entities to biologically coherent diseases defined by molecular alterations. The 2021 WHO Classification of Tumors of the Central Nervous System and its 2022 update formalize this shift, establishing integrated diagnosis as the global ...
Maria Guarnaccia, Sebastiano Cavallaro
wiley   +1 more source

A simplified and rapid in situ hybridization protocol for planarians

BioTechniques, 2023
Whole-mount in situ hybridization is a critical technique for analyzing gene expression in planarians. While robust in situ protocols have been developed, these protocols are laborious, making them challenging to incorporate in an academic setting ...
Andrew J Gaetano, Ryan S King
doaj   +1 more source

Clinical Impact of MGMT Promoter Methylation in IDH‐Mutant Gliomas: Influence of Threshold Selection and Clinical Confounding

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background The clinical relevance of MGMT promoter methylation in IDH‐mutant gliomas remains controversial in the era of molecular classification. We aimed to systematically evaluate its clinical relevance by integrating quantitative assessment, cutoff exploration, and adjustment for clinical confounding.
Haihui Jiang, Linxi Cao, Xiaodong Chen, Siqi Tong, Qinghui Zhu, Jun Yang, Song Lin, Yong Cui   +7 more
wiley   +1 more source